Dietary arginine supplementation enhances intestinal expression of SLC7A7 and SLC7A1 and ameliorates growth depression in mycotoxin-challenged pigs

Abstract

This study tested the hypothesis that dietary L-arginine supplementation confers beneficial effects on growing pigs fed a mold-contaminated diet. The measured variables included: (1) the average daily weight gain and feed:gain ratio; (2) activities of total superoxide dismutase, glutathione peroxidase, diamine oxidase, as well as amino acid and D-lactate concentrations in serum; (3) intestinal morphology; (4) expression of the genes for SLC7A7 (amino acid transporter light chain, y(+L) system, family 7, member 7), SLC7A1 (cationic amino acid transporter, y(+) system, family 7, member 1), SLC1A1 (neuronal/epithelial high affinity glutamate transporter, system XAG, member 1), SLC5A1 (sodium/glucose cotransporter, family 5, member 1) in the ileum and jejunum. Mycotoxins in feedstuffs resulted in an enlarged small intestine mass, oxidative injury in tissues, and reduced growth performance in pigs. Dietary arginine supplementation enhanced (P < 0.05) expression of jejunal SLC7A7 and ileal SLC7A1, in comparison with the control and mycotoxin groups. In addition, supplementing 1% L-arginine to the mycotoxin-contaminated feed had the following beneficial effects (P < 0.05): (1) alleviating the imbalance of the antioxidant system in the body; (2) ameliorating intestinal abnormalities; and (3) attenuating whole-body growth depression, compared with the mycotoxin group without arginine treatment. Collectively, these results indicate that dietary supplementation with L-arginine exerts a protective role in pigs fed mold-contaminated foods. The findings may have important nutritional implications for humans and other mammals.

Preinduction of heat shock protein 70 protects mice against post-infection irritable bowel syndrome via NF-κB and NOS/NO signaling pathways

“…Upregulation of HSP70 markedly reversed the reduced serum arginine and ileal SCL7A7 mRNA. Arginine plays important roles as metabolic intermediates in nutrition, immune response, inflammation, and protein synthesis (Yin et al 2014(Yin et al , 2015b. Several reports indicated that arginine serves as a mediator for HSP70 expression (Wu et al 2013b) and dietary supplementation with arginine upregulates HSP70 expression in animals ) and cells (Pedrycz and Siermontowski 2013).…”

“…NO was measured as released NO metabolites (nitrates and nitrites). Amino acids in serum were determined by LC-MS/MS (HPLC Ultimate 3000 and 3200 QTRAP LC-MS/MS) using standards from Sigma Chemicals (St. Louis, MO, USA) according to previous report (Yin et al 2014). …”

El comportamiento bioproductivo de cerdas reproductoras y su descendencia alimentadas con aditivo probiótico

“…Por lo tanto, el uso de los probióticos es una buena alternativa para mantener un equilibrio en la microbiota natural en lechones neonatos. Así mismo, la salud de los animales se ve influenciada por las condiciones ambientales y de manejo durante el destete, donde sufre cambios drásticos en su régimen social y alimentación, que desencadenan en una serie de cambios morfológicos (a nivel epitelial) y fisioló-gicos, que lo hacen susceptible a enfermedades de tipo bacteriano (Yin et al, 2014;Lähteinen et al, 2015).…”

“…En la última década la industria porcina se ha preocupado por la eficiencia productiva, aumentando el número de crías por hembra al año a un costo menor, lo cual implica el acortamiento de los días de lactancia, así como el tiempo del destete en plena etapa de desarrollo fisiológico de los lechones (Yin et al, 2014). Dicha situación conlleva al tardío establecimiento de la microbiota natural en el tracto digestivo, que provoca la consecuente aparición de enfermedades entéricas, debido a la rápida colonización de microorganismos patógenos en dichos animales (Chaytor et al, 2011;Ihara et al, 2013).…”

Long-term effects of oral tea polyphenols and Lactobacillus brevis M8 on biochemical parameters, digestive enzymes, and cytokines expression in broilers

“…β-Actin was used as a house keeping gene to normalize target gene transcript levels (forward: 5'-CAT CCT GCG TCT GGA CCT GG-3'; reserve: 5'-TAA TGT CAC GCA CGA TTT CC-3'). Real-time PCR was performed according to previous studies (Yin et al, 2014b;2015a). Relative expression was normalized and expressed as a ratio to the expression in the control group.…”