Dietary administration of probiotic Paenibacillus ehimensis NPUST1 with bacteriocin-like activity improves growth performance and immunity against Aeromonas hydrophila and Streptococcus iniae in Nile tilapia (Oreochromis niloticus)

Chen, Sai-Wei; Liu, Chun-Hung; Hu, Shao‐Yang

doi:10.1016/j.fsi.2018.10.059

Cited by 82 publications

(40 citation statements)

References 38 publications

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“…The emerging trend of the development of bacteriocins as alternatives to antibiotics or immunostimulators is accompanied by the rapid expansion of studies on isolation, genetics, engineering production, and potential applications of bacteriocins [4]. In our previous study, we reported that P. ehimensis NPUST1 exhibited bacteriocin-like activity and demonstrated the immunomodulatory and disease resistance effects of this organism on tilapia ( Oreochromis noltica ) [19]. In the present study, the bacteriocin-like substance peocin was identified as a DNA starvation/stationary phase protection protein by gel overlay and LC-MS/MS analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Anaerobic pathogens Porphyromonas gingivalis (BCRC 14417), Propionbacterium acnes (BCRC 10723) and Prevotella nigrescens (BCRC 14408) were purchased from the Bioresource Collection and Research Center (BCRC) (Hsinchu, Taiwan), and the culture conditions and protocols followed the instruction manual. The source of the other pathogens and the culture methods used in this study are described in a previous report [19].…”

Section: Methodsmentioning

confidence: 99%

“…The protocol for cultivation of P. ehimensis NPUST1 was described previously in [19]. The bacteriocin-like substance from cell-free culture broth after 96 h of cultivation was partially purified by ammonium sulfate precipitation.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, a report showed that the production yield of BtCspB, a novel cold-shock-like bacteriocin from Bacillus thuringiensis , by heterologous expression in recombinant E. coli significantly increased 449 times, supporting the use of E. coli as an effective system for bacteriocin production [18]. In our previous study, we isolated the probiotic Paenibacillus ehimensis NPUST1 with bacteriocin-like activity from tilapia culture ponds and demonstrated that the bacteriocin-like substance peocin exhibited heat stability, pH tolerance, and antagonistic activity against diverse aquatic pathogens, food spoilage bacteria, clinical pathogens, plant pathogens, and multiple antibiotic-resistant pathogens [19]. The present study aimed to characterize the bacteriocin peocin and optimize heterologous expression of peocin in E. coli .…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization and Heterologous Production of the Bacteriocin Peocin, a DNA Starvation/Stationary Phase Protection Protein, from Paenibacillus ehimensis NPUST1

et al. 2019

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The production of a bacteriocin-like substance with antimicrobial activity, named peocin, by the probiotic Paenibacillus ehimensis NPUST1 was previously reported by our laboratory. The present study aimed to identify peocin and increase the peocin yield by heterologous expression in Escherichia coli BL21(DE3). Peocin was identified as a DNA starvation/stationary phase protection protein, also called DNA-binding protein from starved cells (Dps), by gel overlay and LC-MS/MS analysis. For mass production of peocin, fed-batch cultivation of E. coli was performed using a pH-stat control system. Purification by simple nickel affinity chromatography and dialysis yielded 45.3 mg of purified peocin from a 20-mL fed-batch culture (49.3% recovery). The biological activity of the purified peocin was confirmed by determination of the MIC and MBC against diverse pathogens. Purified peocin exhibited antimicrobial activity against aquatic, food spoilage, clinical and antibiotic-resistant pathogens. In an in vivo challenge test, zebrafish treated with purified peocin exhibited significantly increased survival rates after A. hydrophila challenge. The present study is the first to show the antimicrobial activity of Dps and provides an efficient strategy for production of bioactive peocin, which will aid the development of peocin as a novel antimicrobial agent with potential applications in diverse industries.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization and Heterologous Production of the Bacteriocin Peocin, a DNA Starvation/Stationary Phase Protection Protein, from Paenibacillus ehimensis NPUST1

et al. 2019

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“…The protocol for screening potential probiotics was modified in accordance with the previous report of Chen et al . (2019). In brief, faeces were collected from day‐old pigeons and serially diluted ten times with sterile water.…”

Section: Methodsmentioning

confidence: 99%

Probiotic supplementation containing Bacillus velezensis enhances expression of immune regulatory genes against pigeon circovirus in pigeons ( Columba livia )

Tsai

Santos

et al. 2020

J Appl Microbiol

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Aims In this study, we aimed to isolate and evaluate the efficacy of Bacillus velezensis as a probiotic and to assess its activity towards pigeons infected with pigeon circovirus (PiCV). Methods and Results Bacillus velezensis, isolated from pigeon faeces, was orally administered to pigeons for 60 days. After pigeons were challenged with PiCV, the PiCV viral load and expression of indicator genes for innate immunity were detected in spleen tissue and faeces of pigeons. Bacillus velezensis significantly reduced the PiCV viral load in the faeces and spleen of pigeons 5 days post‐challenge (dpc). The mRNA expression levels of treated pigeons showed that interferon‐gamma (IFN‐γ), myxovirus resistance 1 (Mx1), and signal transducers and activators of transcription 1 (STAT1) genes were upregulated, whereas no expression of interleukin‐4 (IL‐4) was detected. Moreover, toll‐like receptor 2 (TLR2) and 4 (TLR4) were significantly upregulated in probiotic‐treated pigeons (P < 0·05). Conclusions This is the first report showing that probiotic supplementation can effectively enhance the T‐helper type 1 immune response and decrease the PiCV viral loads in pigeons. Significance and Impact of the Study This study proposes that the administration of a probiotic strain, B. velezensis, to pigeons can protect against PiCV infection.

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Microbiome, Epigenetics and Fish Health Interactions in Aquaculture

Consuegra,

Webster,

Anka

2023

Epigenetics in Aquaculture

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Dietary administration of probiotic Paenibacillus ehimensis NPUST1 with bacteriocin-like activity improves growth performance and immunity against Aeromonas hydrophila and Streptococcus iniae in Nile tilapia (Oreochromis niloticus)

Cited by 82 publications

References 38 publications

Molecular Characterization and Heterologous Production of the Bacteriocin Peocin, a DNA Starvation/Stationary Phase Protection Protein, from Paenibacillus ehimensis NPUST1

Molecular Characterization and Heterologous Production of the Bacteriocin Peocin, a DNA Starvation/Stationary Phase Protection Protein, from Paenibacillus ehimensis NPUST1

Probiotic supplementation containing Bacillus velezensis enhances expression of immune regulatory genes against pigeon circovirus in pigeons ( Columba livia )

Microbiome, Epigenetics and Fish Health Interactions in Aquaculture

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