Diet controls normal and tumorous germline stem cells via insulin-dependent and -independent mechanisms in Drosophila

Hsu, Hwei-Jan; LaFever, Leesa; Drummond‐Barbosa, Daniela

doi:10.1016/j.ydbio.2007.11.006

Cited by 163 publications

(230 citation statements)

References 68 publications

Supporting

Mentioning

211

Contrasting

Order By: Relevance

“…High levels of CYE-1 are also observed in other rapidly dividing cells that lack a significant G1 phase, including mouse embryonic stem cells (Stead et al, 2002) and early Drosophila and Xenopus embryos (Richardson et al, 1993;Rempel et al, 1995). Importantly in Drosophila, the male germline stem cell/cystoblast daughter lacks a detectable G1 phase under normal conditions (Yukiko Yamashita, personal communication), and the female germline stem cell has a short G1 with high cyclin E in G2 and M phase (Hsu et al, 2008). Thus, an atypical cell cycle structure with a short or no G1 and high cyclin E levels may be common in germline stem cell systems.…”

Section: Discussionmentioning

confidence: 99%

“…In animals, nutrient deprivation is not the signal for meiotic entry. Instead, nutrient deprivation can affect the rate of stem cell proliferation, at least in part through insulin signaling (Drummond-Barbosa and Spradling, 2001;Hsu et al, 2008;Michaelson et al, 2010), with meiotic entry controlled by developmental pathways (Crittenden et al, 2002;Hansen et al, 2004b;Matson et al, 2010). Nevertheless, in animals, control of the mitotic cell cycle and core cell cycle regulators is also likely to be important in the switch from proliferation to meiosis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cyclin E and CDK-2 regulate proliferative cell fate and cell cycle progression in the C. elegans germline

et al. 2011

View full text Add to dashboard Cite

SUMMARYThe C. elegans germline provides an excellent model for analyzing the regulation of stem cell activity and the decision to differentiate and undergo meiotic development. The distal end of the adult hermaphrodite germline contains the proliferative zone, which includes a population of mitotically cycling cells and cells in meiotic S phase, followed by entry into meiotic prophase. The proliferative fate is specified by somatic distal tip cell (DTC) niche-germline GLP-1 Notch signaling through repression of the redundant GLD-1 and GLD-2 pathways that promote entry into meiosis. Here, we describe characteristics of the proliferative zone, including cell cycle kinetics and population dynamics, as well as the role of specific cell cycle factors in both cell cycle progression and the decision between the proliferative and meiotic cell fate. Mitotic cell cycle progression occurs rapidly, continuously, with little or no time spent in G1, and with cyclin E (CYE-1) levels and activity high throughout the cell cycle. In addition to driving mitotic cell cycle progression, CYE-1 and CDK-2 also play an important role in proliferative fate specification. Genetic analysis indicates that CYE-1/CDK-2 promotes the proliferative fate downstream or in parallel to the GLD-1 and GLD-2 pathways, and is important under conditions of reduced GLP-1 signaling, possibly corresponding to mitotically cycling proliferative zone cells that are displaced from the DTC niche. Furthermore, we find that GLP-1 signaling regulates a third pathway, in addition to the GLD-1 and GLD-2 pathways and also independent of CYE-1/CDK-2, to promote the proliferative fate/inhibit meiotic entry.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cyclin E and CDK-2 regulate proliferative cell fate and cell cycle progression in the C. elegans germline

et al. 2011

View full text Add to dashboard Cite

show abstract

“…18,19,21 The O. dioica Cyclin D variants, Da, Db, Dc and Dd are encoded by 4 different genes. 24 Previously, we have shown that O. dioica Cyclin Dd regulates G1-phase in mitotically proliferating cells during early development and in post-metamorphic somatic endocycling cells.…”

Section: Asynchronously Proliferating O Dioica Germ Nuclei Have a DImentioning

confidence: 99%

“…In invertebrate Drosophila and C. elegans GSCs, G1 phase is nearly absent. [18][19][20][21] In this context, cell cycle profiles of Cyclin E-CDK activity are altered with Cyclin E present throughout the GSC cell cycle. 18,21 In these invertebrates, proliferative renewal of GSCs is nonetheless still responsive to insulin and TOR signaling 2,22 but these nutrient-dependent controls operate during G2, as opposed to G1.…”

Section: Introductionmentioning

confidence: 99%

Co-expressed Cyclin D variants cooperate to regulate proliferation of germline nuclei in a syncytium

2015

View full text Add to dashboard Cite

The role of the G1-phase Cyclin D-CDK 4/6 regulatory module in linking germline stem cell (GSC) proliferation to nutrition is evolutionarily variable. In invertebrate Drosophila and C. elegans GSC models, G1 is nearly absent and Cyclin E is expressed throughout the cell cycle, whereas vertebrate spermatogonial stem cells have a distinct G1 and Cyclin D1 plays an important role in GSC renewal. In the invertebrate, chordate, Oikopleura, where germline nuclei proliferate asynchronously in a syncytium, we show a distinct G1-phase in which 2 Cyclin D variants are co-expressed. Cyclin Dd, present in both somatic endocycling cells and the germline, localized to germline nuclei during G1 before declining at G1/S. Cyclin Db, restricted to the germline, remained cytoplasmic, co-localizing in foci with the Cyclin-dependent Kinase Inhibitor, CKIa. These foci showed a preferential spatial distribution adjacent to syncytial germline nuclei at G1/S. During nutrient-restricted growth arrest, upregulated CKIa accumulated in arrested somatic endoreduplicative nuclei but did not do so in germline nuclei. In the latter context, Cyclin Dd levels gradually decreased. In contrast, the Cyclin Dbb splice variant, lacking the Rb-interaction domain and phosphodegron, was specifically upregulated and the number of cytoplasmic foci containing this variant increased. This upregulation was dependent on stress response MAPK p38 signaling. We conclude that under favorable conditions, Cyclin Dbb-CDK6 sequesters CKIa in the cytoplasm to cooperate with Cyclin Dd-CDK6 in promoting germline nuclear proliferation. Under nutrient-restriction, this sequestration function is enhanced to permit continued, though reduced, cycling of the germline during somatic growth arrest.

show abstract

“…Each GSC carries a unique organelle with a membranous-like structure, called a fusome, which is juxtaposed to the interface between the cap cell and the GSC. During the late G2/M, S, and early G2 phases of the GSC cell cycle, fusomes display a round, elongated, and exclamation point shape, respectively ( Figure 1B) [13,14]. A single GSC division gives rise to a cystoblast, which undergoes four rounds of incomplete division to form a 16-cell cyst; the cells of the cyst are interconnected with a branched fusome [12].…”

mentioning

confidence: 99%

Age Regulates Drosophila Ovarian Germline Stem Cells via Insulin-Dependent and Independent Mechanisms

Chou¹,

Hsu²

2017

J Cell Signal

View full text Add to dashboard Cite

Diet controls normal and tumorous germline stem cells via insulin-dependent and -independent mechanisms in Drosophila

Cited by 163 publications

References 68 publications

Cyclin E and CDK-2 regulate proliferative cell fate and cell cycle progression in the C. elegans germline

Cyclin E and CDK-2 regulate proliferative cell fate and cell cycle progression in the C. elegans germline

Co-expressed Cyclin D variants cooperate to regulate proliferation of germline nuclei in a syncytium

Age Regulates Drosophila Ovarian Germline Stem Cells via Insulin-Dependent and Independent Mechanisms

Contact Info

Product

Resources

About