“…The sterilized leaf explants from crown and those from organogenesis obtaining plantlets were excised aseptically and cut into pieces of 1 to 2 cm before being cultured on MS medium (1962) basal salts (MSBS) supplemented with 30 g/l of sucrose, vitamins B5, glycine (2.0 mg/l), glutamine (1,000 mg/l), casein hydrolysate (100 mg/l) and MgCl2 (0.75 g/l)-(Sigma, France). [19] The media were different by supplying BAP (0.5 mg/l or 1 mg/l), Kinetin (0.5 mg/l or 1 mg/l) and copper (2 mg/l). Each cytokinin were combined with 2,4-D or Picloram at the concentrations of 3 mg/l, 6 mg/l, 9 mg/l or 12 mg/l.…”