Dielectrophoretic trapping in microwells for manipulation of single cells and small aggregates of particles

Bocchi, Massimo; Lombardini, Marta; Faenza, A; Rambelli, Laura; Giulianelli, Luca; Pecorari, Nicola; Guerrieri, R.

doi:10.1016/j.bios.2008.07.014

Cited by 50 publications

(34 citation statements)

References 15 publications

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“…A simple method for producing microwell DEP traps has been described by Fatoyinbo et al 115 and involves drilling holes through a laminate consisting of 20 aluminum layers and 19 epoxy layers. Bocchi et al 116 describe a similar method that involves drilling holes through a polyimide substrate containing copper-gold or aluminum metal layers that form three annular electrodes within the well. A channel under the device provides the means for fluid flow into the microwells by capillary action.…”

Section: Technologymentioning

confidence: 99%

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

2010

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A review is presented of the present status of the theory, the developed technology and the current applications of dielectrophoresis ͑DEP͒. Over the past 10 years around 2000 publications have addressed these three aspects, and current trends suggest that the theory and technology have matured sufficiently for most effort to now be directed towards applying DEP to unmet needs in such areas as biosensors, cell therapeutics, drug discovery, medical diagnostics, microfluidics, nanoassembly, and particle filtration. The dipole approximation to describe the DEP force acting on a particle subjected to a nonuniform electric field has evolved to include multipole contributions, the perturbing effects arising from interactions with other cells and boundary surfaces, and the influence of electrical double-layer polarizations that must be considered for nanoparticles. Theoretical modelling of the electric field gradients generated by different electrode designs has also reached an advanced state. Advances in the technology include the development of sophisticated electrode designs, along with the introduction of new materials ͑e.g., silicone polymers, dry film resist͒ and methods for fabricating the electrodes and microfluidics of DEP devices ͑photo and electron beam lithography, laser ablation, thin film techniques, CMOS technology͒. Around three-quarters of the 300 or so scientific publications now being published each year on DEP are directed towards practical applications, and this is matched with an increasing number of patent applications. A summary of the US patents granted since January 2005 is given, along with an outline of the small number of perceived industrial applications ͑e.g., mineral separation, micropolishing, manipulation and dispensing of fluid droplets, manipulation and assembly of micro components͒. The technology has also advanced sufficiently for DEP to be used as a tool to manipulate nanoparticles ͑e.g., carbon nanotubes, nano wires, gold and metal oxide nanoparticles͒ for the fabrication of devices and sensors. Most efforts are now being directed towards biomedical applications, such as the spatial manipulation and selective separation/enrichment of target cells or bacteria, high-throughput molecular screening, biosensors, immunoassays, and the artificial engineering of three-dimensional cell constructs. DEP is able to manipulate and sort cells without the need for biochemical labels or other bioengineered tags, and without contact to any surfaces. This opens up potentially important applications of DEP as a tool to address an unmet need in stem cell research and therapy.

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Section: Technologymentioning

confidence: 99%

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

2010

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“…In future applications, particle or cell suspension could continuously flow through the device to achieve higher-throughput DEP sorting 50 . Specific biomedical applications that require sorting of large volumes to separate and identify rare cells include detection of circulating tumor cells 51 and sepsis 52 .…”

Section: Representative Resultsmentioning

confidence: 99%

Development of a 3D Graphene Electrode Dielectrophoretic Device

Xie

Tewari

Fukushima

et al. 2014

JoVE

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The design and fabrication of a novel 3D electrode microdevice using 50 µm thick graphene paper and 100 µm double sided tape is described. The protocol details the procedures to construct a versatile, reusable, multiple layer, laminated dielectrophoresis chamber. Specifically, six layers of 50 µm x 0.7 cm x 2 cm graphene paper and five layers of double sided tape were alternately stacked together, then clamped to a glass slide. Then a 700 μm diameter micro-well was drilled through the laminated structure using a computer-controlled micro drilling machine. Insulating properties of the tape layer between adjacent graphene layers were assured by resistance tests. Silver conductive epoxy connected alternate layers of graphene paper and formed stable connections between the graphene paper and external copper wire electrodes. The finished device was then clamped and sealed to a glass slide. The electric field gradient was modeled within the multi-layer device. Dielectrophoretic behaviors of 6 μm polystyrene beads were demonstrated in the 1 mm deep micro-well, with medium conductivities ranging from 0.0001 S/m to 1.3 S/m, and applied signal frequencies from 100 Hz to 10 MHz. Negative dielectrophoretic responses were observed in three dimensions over most of the conductivity-frequency space and cross-over frequency values are consistent with previously reported literature values. The device did not prevent AC electroosmosis and electrothermal flows, which occurred in the low and high frequency regions, respectively. The graphene paper utilized in this device is versatile and could subsequently function as a biosensor after dielectrophoretic characterizations are complete. Video LinkThe video component of this article can be found at

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“…The authors applied sinusoidal signals on the electrodes at frequencies ranging from 100 kHz to 1.5 MHz and amplitudes between 2 V and 7 V. Particles are successfully trapped and levitated at the level of the central electrode in the middle of microwells with a diameter of 125 μm, shown in Fig. 4 [35].…”

Section: Electrical Manipulation Techniquementioning

confidence: 99%

“…When the particle is less polarizable than the surrounding medium ( f CM < 0) it will experience a n-DEP force pushing it towards regions of electric field minima. On the contrary, when the particle is more polarizable than the medium ( f CM > 0) it will be attracted by a p-DEP force towards regions of field maxima [35]. Based on the theory of DEP, scientists and engineers designed and developed many devices for manipulation cells.…”

Section: Electrical Manipulation Techniquementioning

confidence: 99%

“…A fluid carrier is used to create a channel under the well which is filled by capillarity, while the hydrophobic coating on top side allows the fluid to be kept within the hole and a meniscus consequently to form. A particle can be inserted manually or with the support of a cell dispenser from the top and will be trapped at the level of the second metal layer [35].…”

Section: Electrical Manipulation Techniquementioning

confidence: 99%

See 1 more Smart Citation

Single Cell Manipulation Technology

Lin¹,

Chen²,

Xie³

et al. 2015

Nano BioMed ENG

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With microfluidic technique emerging, cell manipulation technology combines with microfluidic become a promising tool for single-cell-level manipulation. To obtain a kind of or single pure target cell for eliminating interference of useless cells in the trial of molecular biology, genetic analysis, proteomics and single cell analysis, various cell manipulation techniques have been developed for recovery specific cells. In this review, we introduce the principles of each cell manipulation technology and overlook the latest achievements of cell manipulation technique by categorizing externally applied manipulation forces: optical, electrical, magnetic, acoustic, mechanical. We also summarize the advantages and drawbacks of each cell manipulation technique.

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Dielectrophoretic trapping in microwells for manipulation of single cells and small aggregates of particles

Cited by 50 publications

References 15 publications

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

Development of a 3D Graphene Electrode Dielectrophoretic Device

Single Cell Manipulation Technology

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