Dielectrophoretic Separation of Particles Using Microfluidic Chip with Composite Three-Dimensional Electrode

Chen, Li; Liu, Xing; Zheng, Xiaolin; Yang, Jun; Tian, Tian; Liao, Yanjian

doi:10.3390/mi11070700

Cited by 23 publications

(17 citation statements)

References 43 publications

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“…The particle tracing for fluid flow interface solves ordinary differential equations for particle positions. When the Newtonian formulation is selected, the particle position is computed using Newton's second law [26]:

\begin{equation}F = \frac{{d\left( {{m_p}v} \right)}}{{dt}},\end{equation}

where m p is the particle mass, v is the particle velocity, and F is the total force exerted on the particle. Each force is specified directly or is using a susceptibility multiplied by a suitable field.…”

Section: Mathematical Model For Particle Trajectorymentioning

confidence: 99%

In vitro dielectrophoresis of HEK cell migration for stimulating chronic wound epithelialization

et al. 2021

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This article describes a dielectrophoresis (DEP)‐based simulation and experimental study of human epidermal keratinocyte (HEK) cells for wounded skin cell migration toward rapid epithelialization. MyDEP is a standalone software designed specifically to study dielectric particles and cell response to an alternating current (AC) electric field. This method demonstrated that negative dielectrophoresis (NDEP) occurs in HEK cells at a wide frequency range in highly conductive medium. The finite element method was used to characterize particle trajectory based on DEP and drag force. The performance of the system was assessed using HEK cells in a highly conductive EpiLife suspending medium. The DEP experiment was performed by applying sinusoidal wave AC potential at the peak‐to‐peak voltage of 10 V in a tapered aluminum microelectrode array from 100 kHz to 1 MHz. We experimentally observed the occurrence of NDEP, which attracted HEK cells toward the local electric field minima in the region of interest. The DIPP‐MotionV software was used to track cell migration in the prerecorded video via an automatic marker and estimate the average speed and acceleration of the cells. The results showed that HEK cell migration was accomplished approximately at 6.43 μm/s at 100 kHz with 10 V, and FDEP caused the cells to migrate and align at the target position, which resulted in faster wound closures because of the application of an electric field frequency to HEK cells in random locations.

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\begin{equation}F = \frac{{d\left( {{m_p}v} \right)}}{{dt}},\end{equation}

Section: Mathematical Model For Particle Trajectorymentioning

confidence: 99%

In vitro dielectrophoresis of HEK cell migration for stimulating chronic wound epithelialization

et al. 2021

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“…Re[K*(ω)] is the real part of the complex Claussius-Mossotti (CM) factor [27,28] , which is given by…”

Section: Theory and Working Principlementioning

confidence: 99%

Separation-enrichment method for airborne disease spores based on microfluidic chip

Zhang

Yang

et al. 2021

International Journal of Agricultural and Biological Engineering

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Airborne diseases are likely to cause crop yield reduction, which has aroused widespread concern. In this study, a two-stage separation-enrichment structure microfluidic chip with a compound field for separation and enrichment of the greenhouse crops airborne disease spores directly from gas flow was developed. The chip is mainly composed of three parts: arc structure pretreatment channel, semicircular electrode structure and collection tank. COMSOL 5.1 software was used to simulate the designed microfluidic chip. 30 µm particles were used to represent P. xanthii spores, 25 µm particles were used to represent P. cubensis spores, and 16 µm particles were used to represent B. cinerea spores. The simulation results showed that the separation and enrichment efficiency of 16 μm particles, 25 μm particles, and 30 μm particles was 88%, 91%, and 94%, respectively. The experimental verification results were observed under a microscope. The results showed that the separation and enrichment efficiency of B. cinereal spores, P. cubensis spores and P. xanthii spores was 75.7%, 83.8% and 89.4%, respectively. As a result, the designed microfluidic chip can be used to separate and enrich the spores of airborne diseases of greenhouse crops, which can provide a basis for the research of real-time monitoring technology for greenhouse airborne diseases.

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“…Chen et al. (2020) used a microfluidic chip combined with a composite three‐dimensional electrode to successfully separate polystyrene particles of three different sizes by adjusting the flow rate and dielectrophoresis force, and COMSOL was used to simulate the movement of the polystyrene particles under the influence of hydrodynamic and dielectrophoretic force.…”

Section: Simulation Of Driving Modesmentioning

confidence: 99%

Computer simulation of submicron fluid flows in microfluidic chips and their applications in food analysis

Xie

Sun

2021

Comp Rev Food Sci Food Safe

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In recent years, countries around the world have maintained a zero‐tolerance attitude toward safety problems in the food industry. In order to ensure human health, a fast, sensitive, and high‐throughput analysis of food contaminants is necessary to ensure safe food products on the market. Microfluidics, as a high‐efficiency and sensitive detection technology, has many advantages in the detection of food contaminants, including foodborne pathogens, pesticides, heavy metal ions, toxic substances, and so forth, especially in conjunction with a variety of submicron fluid driving methods, making food detection and analysis more efficient and accurate. This review introduces the principle of submicron fluid driving modes and discusses the driving simulation of submicron fluid in microfluidic chips. In addition, the latest developments in the application of simulation in food analysis from 2006 to 2020 are discussed, and the computer simulation of submicron fluid flow in microfluidic chips and its application and development trend in food analysis are also highlighted. The review indicates that microfluidic technology, using numerical simulation as an auxiliary tool, combined with traditional methods has greatly improved the detection and analysis of food products. In addition, microfluidics combined with a variety of control methods embodies the ability of specific, multifunctional, and sensitive detection and analysis of food products. The development of high‐sensitivity, high‐throughput, portable, integrated microfluidic chips will enable the technology to be applied in practice.

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Dielectrophoretic Separation of Particles Using Microfluidic Chip with Composite Three-Dimensional Electrode

Cited by 23 publications

References 43 publications

In vitro dielectrophoresis of HEK cell migration for stimulating chronic wound epithelialization

In vitro dielectrophoresis of HEK cell migration for stimulating chronic wound epithelialization

Separation-enrichment method for airborne disease spores based on microfluidic chip

Computer simulation of submicron fluid flows in microfluidic chips and their applications in food analysis

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