Dielectrophoretic Recovery of DNA from Plasma for the Identification of Chronic Lymphocytic Leukemia Point Mutations

Manouchehri, Sareh; Ibsen, Stuart; Wright, Jennifer; Rassenti, Laura Z.; Ghia, Emanuela M.; Widhopf, George F.; Kipps, Thomas J.; Heller, Michael

doi:10.2217/ijh-2015-0009

Cited by 21 publications

(23 citation statements)

References 26 publications

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“…None of these solutions are ideal. This said, examples of commercial planar electrode arrays (Biological Dynamics, San Diego, CA), are seen in , which shows that this architecture has found success with high conductance solutions, and provides an empirical reference for further designs. Nevertheless, even these devices, designed to operate with high conductance solutions, have performance limitations due to adverse electrochemical effects when driven above recommended voltages or below recommended frequency of operation.…”

Section: Introductionmentioning

confidence: 93%

“…Electrokinetic separation devices offer tremendous promise in terms of miniaturization and direct separation of targeted biomarkers without the need to go through centrifugation, chemical isolation, and precipitation steps. New devices, which can operate under high conductance conditions (>0.5 S/m), have now been used to isolate cancer related cf‐DNA and drug delivery nanoparticles from un‐diluted blood and plasma samples. There is also research into using DEP to concentrate relevant biomarkers for chemical assay .…”

Section: Introductionmentioning

confidence: 99%

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Electrokinetic device design and constraints for use in high conductance solutions

2017

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The quest for new cell-free DNA and exosome biomarker-based molecular diagnostics require fast and efficient sample preparation techniques. Conventional methods for isolating these biomarkers from blood are both time-consuming and laborious. New electrokinetic microarray devices using dielectrophoresis (DEP) to isolate cell-free DNA and exosome biomarkers have now greatly improved the sample preparation process. Nevertheless, these devices still have some limitations when used with high conductance biological fluids, e.g. blood, plasma, and serum. This study demonstrates that electrochemical damage may occur on the platinum electrodes of DEP microarray devices. It further examines two model device designs that include a parallel wire arrangement and a planar array. Effective isolation of fluorescent beads with parallel wires is shown under low-conductance conditions (10 S/m), but electrothermal flow overcomes DEP forces under high conductance conditions (>0.1 S/m). Planar devices are shown to be effective under high conductance conditions (∼1 S/m) without the deleterious effects of electrothermal flow. This study provides new insights into design compromises and limitations for producing future electrokinetic devices for better performance with high conductance solutions.

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Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Electrokinetic device design and constraints for use in high conductance solutions

2017

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“…The steps during the preparations, such as pipetting, centrifuging and filtrating, would sometimes degrade the DNA quality and quantity [166,169,172]. The purification, recovery and isolation from whole-blood samples would also be a major challenge in DNA research [166,167,169]. Furthermore, a common electroporation instrument such as an optical tweezer will damage the cell membrane during the transfection [165].…”

Section: Dep Applications In Biomedical Sciencesmentioning

confidence: 99%

“…The electrotransfection have used a low applied AC voltage to enable electroporation and transfection. Moreover, researchers have implemented a DEP-based microarray chip for extracting circulating DNA [166,167,169]. On the other hand, DEP force, generated by two combine electrodes stretching system, was used to immobilized λ DNA molecules [168].…”

Section: Dep Applications In Biomedical Sciencesmentioning

confidence: 99%

Dielectrophoresis for Biomedical Sciences Applications: A Review

Rahman

Ibrahim

Yafouz

2017

Sensors

158

152

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Dielectrophoresis (DEP) is a label-free, accurate, fast, low-cost diagnostic technique that uses the principles of polarization and the motion of bioparticles in applied electric fields. This technique has been proven to be beneficial in various fields, including environmental research, polymer research, biosensors, microfluidics, medicine and diagnostics. Biomedical science research is one of the major research areas that could potentially benefit from DEP technology for diverse applications. Nevertheless, many medical science research investigations have yet to benefit from the possibilities offered by DEP. This paper critically reviews the fundamentals, recent progress, current challenges, future directions and potential applications of research investigations in the medical sciences utilizing DEP technique. This review will also act as a guide and reference for medical researchers and scientists to explore and utilize the DEP technique in their research fields.

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“…Thus, for a heterogeneous particle population, we can determine a range of frequencies where one particle subpopulation experiences pDEP while the other nDEP, allowing separation. DEP has been used to distinguish a wide variety of particles, including leukocytes from erythrocytes , leukocyte subpopulations , erythrocyte subpopulations (based on ABO‐Rh type) , cancer cell lines , various species of bacteria , cellular components , and even viruses and macromolecules . For current state‐of‐the‐art of DEP separations, see .…”

Section: Introductionmentioning

confidence: 99%

Continuous dielectrophoretic particle separation via isomotive dielectrophoresis with bifurcating stagnation flow

2019

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We present a novel technique for continuous label‐free separation of particles based on their dielectrophoretic crossover frequencies. Our technique relies on our unique microfluidic geometry which performs hydrodynamic focusing, generates a stagnation flow with two outlets, and simultaneously produces an isomotive dielectrophoretic field via wall‐situated electrodes. To perform particle separation, we hydrodynamically focus particles onto stagnation streamlines and use isomotive dielectrophoretic force to nudge the particles off these streamlines and direct them into appropriate outlets. Focusing particles onto stagnation streamlines obviates the need for large forces to be applied to the particles and therefore increases system throughput. The use of isomotive (spatially uniform) dielectrophoretic force increases system reliability. To guide designers, we develop and describe a simple scaling model for the particle separation dynamics of our technique. The model predicts the range of particle sizes that can be separated as well as the processing rate that can be achieved as a function of system design parameters: channel size, flow rate, and applied potential. Finally, as a proof‐of‐principle, we use this technique to separate polystyrene bead and cell mixtures of the same diameters as well as mixtures of both particles with varying diameters.

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Dielectrophoretic Recovery of DNA from Plasma for the Identification of Chronic Lymphocytic Leukemia Point Mutations

Cited by 21 publications

References 26 publications

Electrokinetic device design and constraints for use in high conductance solutions

Electrokinetic device design and constraints for use in high conductance solutions

Dielectrophoresis for Biomedical Sciences Applications: A Review

Continuous dielectrophoretic particle separation via isomotive dielectrophoresis with bifurcating stagnation flow

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