Dielectrophoresis of malignant human melanocytes

Mischel, Maja; Rougé, Françoise; Lamprecht, I.; Aubert, C; Prota, Giuseppe

doi:10.1007/bf00510042

Cited by 8 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…80 Aer this, DEP equipment has been mainly used in the enrichment and isolation of yeast cells. Since 1982, when human malignant melanocytes were rst successfully arranged by DEP, 81 DEP has been widely used in the manipulation of tumor cells. In 1984, the blood cells obtained from patients suffering from sickle cell anemia were rst used to distinguish normal red blood cells 85 Pd, 86 CdTe, 87 Silica, 88 PMMA, 72 and sickle cells by DEP.…”

Section: Applicationmentioning

confidence: 99%

A review of polystyrene bead manipulation by dielectrophoresis

Chen

Yuan

2019

RSC Adv.

View full text Add to dashboard Cite

show abstract

Section: Applicationmentioning

confidence: 99%

A review of polystyrene bead manipulation by dielectrophoresis

Chen

Yuan

2019

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…Early DEP characterizations were based on observations of cell behavior under an applied field. For example, malignant myocytes were characterized by their pearl‐chaining ability and mean chain length as a function of frequency [66]. Dielectric coefficients, measured from the dielectrophoretic velocities of single cells characterized myeloma, hybridomas, and B‐lymphocytes based on their type, age, and drug treatment as well as chloroplasts untreated and treated with cationized ferritin demonstrating differences in surface charges [67,68].…”

Section: Dep Characterization Methods and Parametersmentioning

confidence: 99%

Review: Dielectrophoresis in cell characterization

Henslee

2020

Electrophoresis

View full text Add to dashboard Cite

Many cellular functions are affected by and thus can be characterized by a cell's electrophysiology. This has also been found to correspond to other biophysical parameters such as cell morphology and mechanical properties. Dielectrophoresis (DEP) is an electrostatic technique which can be used to examine cellular biophysical parameters through the measuring of single or multiple cell response to electric field induced forces. This label‐free method offers many advantages in characterizing a cell population over conventional electrophysiology methods such as patch clamping; however, it has yet to see mainstream pharmacological application. Challenges such as the transdisciplinary nature of the field bridging engineering and the biological sciences, throughput, specificity as well as standardization are being addressed in current literature. This review focuses on the developments of DEP‐based cell electrophysiological characterization where determining cellular properties such as membrane conductance and capacitance, and cytoplasmic conductivity are the primary motivation. A brief theoretical review, techniques for obtaining these cell parameters, as well as the resulting cell parameters and their applications are included in this review. This review aims to further support the development of DEP‐based cell characterization as an important part of the future of DEP and electrophysiology research.

show abstract

“…Human skin cancer cells have specific electrical properties that facilitate separation when using DEP. One important aspect revealed since the earliest experiments [109], is the role of melanin content in modulating melanoma cells' behavior in a non-uniform electrical field. Modifications in cell structure due to normal ageing or destructive changes under different drug therapies will alter the cell movement under DEP, facilitating or impeding single cell separation.…”

Section: Photoacoustic Flow Cytometrymentioning

confidence: 99%

Liquid Biopsy and Dielectrophoretic Analysis—Complementary Methods in Skin Cancer Monitoring

et al. 2022

View full text Add to dashboard Cite

The incidence and prevalence of skin cancers is currently increasing worldwide, with early detection, adequate treatment, and prevention of recurrences being topics of great interest for researchers nowadays. Although tumor biopsy remains the gold standard of diagnosis, this technique cannot be performed in a significant proportion of cases, so that the use of alternative methods with high sensitivity and specificity is becoming increasingly desirable. In this context, liquid biopsy appears to be a feasible solution for the study of cellular and molecular markers relevant to different types of skin cancers. Circulating tumor cells are just one of the components of interest obtained from performing liquid biopsy, and their study by complementary methods, such as dielectrophoresis, could bring additional benefits in terms of characterizing skin tumors and subsequently applying personalized therapy. One purpose of this review is to demonstrate the utility of liquid biopsy primarily in monitoring the most common types of skin tumors: basal cell carcinoma, squamous cell carcinoma, and malign melanoma. In addition, the originality of the article is based on the detailed presentation of the dielectrophoretic analysis method of the most important elements obtained from liquid biopsy, with direct impact on the clinical and therapeutic approach of skin tumors.

show abstract

Dielectrophoresis of malignant human melanocytes

Cited by 8 publications

References 5 publications

A review of polystyrene bead manipulation by dielectrophoresis

A review of polystyrene bead manipulation by dielectrophoresis

Review: Dielectrophoresis in cell characterization

Liquid Biopsy and Dielectrophoretic Analysis—Complementary Methods in Skin Cancer Monitoring

Contact Info

Product

Resources

About