Dieldrin‐dissolving abilities of the xylem saps of several plant families, particularly <i>Cucurbita pepo</i> L.

Murano, Hirotatsu; Otani, Takashi; Seike, Nobuyasu

doi:10.1002/etc.288

Cited by 38 publications

(27 citation statements)

References 35 publications

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“…1). Based on this and previous results (Murano et al, 2010a), it seems that POPs adsorbed on xylem vessels may be desorbed by xylem sap containing some proteins, and then POPs are translocated into the upper parts of plants and accumulated there.…”

Section: Discussionsupporting

confidence: 66%

“…Therefore, xylem sap is thought to be the primary pathway for POP translocation. However, because high transpiration rates were not related to high accumulation in shoots (Murano et al, 2010a), some material(s) with binding activity to POPs within xylem sap might be important. Highly hydrophobic compounds tend to accumulate in the lower parts of C. pepo stems (Aslund et al, 2008), indicating that xylem vessels may trap them on their inner surfaces.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the fact that POPs such as chlordane, dieldrin, and PCBs were detected in xylem sap of C. pepo suggests that their accumulation in the aerial parts of plants occurs during the translocation from roots to aerial parts in xylem sap (Mattina et al, 2004;Murano et al, 2010b;Greenwood et al, 2011). A recent study revealed that there were protein-like materials with the ability to dissolve dieldrin in xylem sap (Murano et al, 2010a). However, these materials have yet to be identified, and the mechanisms underlying the high transport ability and high accumulation of POPs in C. pepo plants are not fully understood.…”

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confidence: 99%

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A Major Latex-Like Protein Is a Key Factor in Crop Contamination by Persistent Organic Pollutants

et al. 2013

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This is the first report, to our knowledge, to reveal important factors by which members of the Cucurbitaceae family, such as cucumber (Cucumis sativus), watermelon (Citrullus lanatus), melon (Cucumis melo), pumpkin (Cucurbita pepo), squash (C. pepo), and zucchini (C. pepo), are selectively polluted with highly toxic hydrophobic contaminants, including organochlorine insecticides and dioxins. Xylem sap of C. pepo ssp. pepo, which is a high accumulator of hydrophobic compounds, solubilized the hydrophobic compound pyrene into the aqueous phase via some protein(s). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of xylem sap of two C. pepo subspecies revealed that the amount of 17-kD proteins in C. pepo ssp. pepo was larger than that in C. pepo ssp. ovifera, a low accumulator, suggesting that these proteins may be related to the translocation of hydrophobic compounds. The protein bands at 17 kD contained major latex-like proteins (MLPs), and the corresponding genes MLP-PG1, MLP-GR1, and MLP-GR3 were cloned from the C. pepo cultivars Patty Green and Gold Rush. Expression of the MLP-GR3 gene in C. pepo cultivars was positively correlated with the band intensity of 17-kD proteins and bioconcentration factors toward dioxins and dioxin-like compounds. Recombinant MLP-GR3 bound polychlorinated biphenyls immobilized on magnetic beads, whereas recombinant MLP-PG1 and MLP-GR1 did not. These results indicate that the high expression of MLP-GR3 in C. pepo ssp. pepo plants and the existence of MLP-GR3 in their xylem sap are related to the efficient translocation of hydrophobic contaminants. These findings should be useful for decreasing the contamination of fruit of the Cucurbitaceae family as well as the phytoremediation of hydrophobic contaminants.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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A Major Latex-Like Protein Is a Key Factor in Crop Contamination by Persistent Organic Pollutants

et al. 2013

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“…Ebisu is a leading commercial variety in Japan and Kurotanenankin is used as a rootstock in Japan. Moreover, Black Tosca is a popular zucchini variety and is reported to take up persistent organic pollutants (POPs) efficiently from soil (Murano et al 2010). Infection efficiency differed among the 3 cultivars as estimated by observation of GFP fluorescence in infected explants ( Figure 4A-G).…”

Section: Transformation Trials For Other Cucurbita Speciesmentioning

confidence: 99%

Improving the transformation efficiency of Cucurbita species: factors and strategy for practical application

Nanasato

Okuzaki

Tabei

2013

Plant Biotechnology

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Cucurbita species are refractory to transformation. Hence, the only 2 reports published regarding the transformation of Cucurbita species until our successful transformation of C. moschata (cv. Heiankogiku) in 2011. The efficiency was 2.7±1.3% using wounded explants vortexed with whisker suspension. To improve transformation efficiency, transformation experiments were carried out with various ages of cotyledonary explants. The highest efficiency was obtained with 1-day-old (3.2±0.9%) and 2-day-old (3.3±0.8%) explants after germination. Histochemical analysis of GUS activity revealed that wounding allowed Agrobacterium access to the deeper layer of explants. These results suggested that cells having a high ability of shoot organogenesis exist not on the surface but in the deeper layers of explants. We applied vacuum infiltration to wounded explants to enhance Agrobacterium access to the deeper layers, which resulted in improvement of transformation efficiency by 3-fold (9.2±2.9%). To verify the efficacy of our transformation procedure for other Cucurbita species, we attempted to obtain transformants with 3 Cucurbita species: C. maxima (cv. Ebisu), C. pepo (cv. Black Tosca), and C. ficifolia (cv. Kurotanenankin). The average regeneration efficiencies were 54.2±7.2%, 62.5±19.1%, and 72.2±13.4% under our regeneration system. Although the efficiency for production of tansgenic plants was very low (ca. 0.2-0.3%), a transgenic line was obtained from C. maxima and C. pepo, respectively. We discuss recent advances that may help in the development of beneficial applications for the transformation of Cucurbita species.

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“…In recent years, species-specific Cucurbita proteins related to POPs uptake and translocation were isolated in xylem saps and their characters investigated (Murano et al 2010;Inui et al 2013). However, even if Cucurbita species were used for phytoremediation of HCH in soils, it would be insufficient for mitigating contamination by POPs, because plants lack enzymes that degrade HCH.…”

Section: Hexachlorocyclohexanementioning

confidence: 99%

Biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of Cucurbita moschata that accumulate recombinant bacterial LinA

et al. 2016

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γ-HCH was successfully degraded using LinA-expressed transgenic hairy root cultures of Cucurbita moschata . Fusing an endoplasmic reticulum-targeting signal peptide to LinA was essential for stable accumulation in the hairy roots. The pesticide γ-hexachlorocyclohexane (γ-HCH) is a persistent organic pollutant (POP) that raises public health and environmental pollution concerns worldwide. Although several isolates of γ-HCH-degrading bacteria are available, inoculating them directly into γ-HCH-contaminated soil is ineffective because of the bacterial survival rate. Cucurbita species incorporate significant amounts of POPs from soils compared with other plant species. Here, we describe a novel bioremediation strategy that combines the bacterial degradation of γ-HCH and the efficient uptake of γ-HCH by Cucurbita species. We produced transgenic hairy root cultures of Cucurbita moschata that expressed recombinant bacterial linA, isolated from the bacterium Sphingobium japonicum UT26. The LinA protein was accumulated stably in the hairy root cultures by fusing an endoplasmic reticulum (ER)-targeting signal peptide to LinA. Then, we demonstrated that the cultures degraded more than 90 % of γ-HCH (1 ppm) overnight and produced the γ-HCH metabolite 1,2,4-trichlorobenzene, indicating that LinA degraded γ-HCH. These results indicate that the gene linA has high potential for phytoremediation of environmental γ-HCH.

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Dieldrin‐dissolving abilities of the xylem saps of several plant families, particularly Cucurbita pepo L.

Cited by 38 publications

References 35 publications

A Major Latex-Like Protein Is a Key Factor in Crop Contamination by Persistent Organic Pollutants

A Major Latex-Like Protein Is a Key Factor in Crop Contamination by Persistent Organic Pollutants

Improving the transformation efficiency of Cucurbita species: factors and strategy for practical application

Biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of Cucurbita moschata that accumulate recombinant bacterial LinA

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