Biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of Cucurbita moschata that accumulate recombinant bacterial LinA

Nanasato, Yoshihiko; Namiki, Sayuri; Oshima, Masao; Moriuchi, Ryota; Konagaya, Ken Ichi; Seike, Nobuyasu; Otani, Takashi; Nagata, Yasunobu; Tsuda, Masashi; Tabei, Yutaka

doi:10.1007/s00299-016-2011-1

Cited by 15 publications

(3 citation statements)

References 52 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, LinA, LinB, and LinC are upstream pathway enzymes of lindane degradation. LinA is a homotetrameric protein with a molecular mass of 16.5 kDa (Nanasato et al, 2016). LinA belongs to the dehydrohalogenase class of enzymes, which can eliminate HCl from a substrate molecule to form a double bond (Cuozzo et al, 2017).…”

Section: Functional Enzymes Involved In Lindane Degradationmentioning

confidence: 99%

Insights Into the Biodegradation of Lindane (γ-Hexachlorocyclohexane) Using a Microbial System

et al. 2020

View full text Add to dashboard Cite

Lindane (γ-hexachlorocyclohexane) is an organochlorine pesticide that has been widely used in agriculture over the last seven decades. The increasing residues of lindane in soil and water environments are toxic to humans and other organisms. Large-scale applications and residual toxicity in the environment require urgent lindane removal. Microbes, particularly Gram-negative bacteria, can transform lindane into non-toxic and environmentally safe metabolites. Aerobic and anaerobic microorganisms follow different metabolic pathways to degrade lindane. A variety of enzymes participate in lindane degradation pathways, including dehydrochlorinase (LinA), dehalogenase (LinB), dehydrogenase (LinC), and reductive dechlorinase (LinD). However, a limited number of reviews have been published regarding the biodegradation and bioremediation of lindane. This review summarizes the current knowledge regarding lindane-degrading microbes along with biodegradation mechanisms, metabolic pathways, and the microbial remediation of lindane-contaminated environments. The prospects of novel bioremediation technologies to provide insight between laboratory cultures and largescale applications are also discussed. This review provides a theoretical foundation and practical basis to use lindane-degrading microorganisms for bioremediation.

show abstract

Section: Functional Enzymes Involved In Lindane Degradationmentioning

confidence: 99%

Insights Into the Biodegradation of Lindane (γ-Hexachlorocyclohexane) Using a Microbial System

et al. 2020

View full text Add to dashboard Cite

show abstract

“…According to the findings of this investigation, LinA appears to be engaged in phytoremediation and hydroxychlorohydrin degradation in the environment. The linA gene was cloned in a hairy root culture of the plant Cucurbita moschata, as demonstrated in this work [35]. [36] genetically engineered the Pseudomonas putida strain KT2440 to achieve full mineralization of the chemicals methyl parathion (MP) and hexachlorocyclohexane (HCH).…”

Section: Plant-microbe Interactionsmentioning

confidence: 99%

The Use of Plants, Nanotechnology and Surfactants in Lindane Remediation: A Mini Review

Uba

Baba

2021

J Biochem Microbiol Biotechnol

View full text Add to dashboard Cite

Lindane is an organochlorine chemical and an isomer of hexachlorocyclohexane that has been used both as an agricultural insecticide and as a pharmaceutical treatment for lice and scabies. Lindane accumulates in the agricultural soil and plants thereby causing environmental and health deteriorative effects. A lot of soil remediation methods used are highly expensive and require a lot of expertise. Phytoremediation (rhizofiltration, phytostabilization), which involve the use of the plant to stabilize or remove environmental toxicants is presently much in use because of its cost-effectiveness and ecological friendliness. Adsorption has emerged as the most efficient, easy, and promising nanotechnology method of wastewater treatment out of the several approaches now employed. Recently, biosurfactants are used for the production of nanoparticles which will be further applied in the area of pesticide remediation. The use of phytoremediation is the most important fully green approach as no new chemicals are added to the polluted soil. Other emerging technologies where phytoremediation might not be applicable especially groundwater has begun to evaluate the use of green nanobiotechnology.

show abstract

“…Nonetheless, the over-application of these chemicals inhibits some of these natural processes and decreases the performance of the non-target organisms [7]. However, some soil organisms use these herbicides in the process of degradation as a carbon energy source for their metabolic activities [8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Effect of Atrazine, 2,4-D Amine, Glyphosate and Paraquat Herbicides on Soil Microbial Population

Abdullahi¹,

Saleh²,

Mzungu³

2022

J Environ Microbiol Toxicol

View full text Add to dashboard Cite

Herbicides’ toxic impact on the non-target soil microorganisms which play roles in degrading organic matter, nitrogen and nutrient recycling and decomposition needs to be considered. In the present study, the effect of four (4) most commonly used herbicides, viz; atrazine, 2, 4- D amine, glyphosate and paraquat on soil microorganisms was assessed over a period of fifteen continuous days (exposure period). The herbicide treatments were the normal recommended field rate, (6.67 mg active ingredient per gram of soil for atrazine, 6.17 mg for 2, 4-D amine, 5.56 mg for glyphosate, and 2.46 mg for paraquat), half and double of the recommended field rates. Tables 4.1 through 4.5 showed the various heterotrophic bacterial colony counts obtained from the various treatments, ranging from the control (Day 1; no herbicide applied) to days 3, 6, 9 and 15 after the application of the various herbicides, respectively. Two general trends are readily observed: the first is that, upon application of the herbicide, the microbial growth steadily diminishes, up to the 6th day. However, from there it continues to increase till the end of the experiments (day 15). This applies to each herbicide. Firstly, the effect of the addition of the various herbicides was evaluated using two samples, a two-tailed Z-Test for means, at a 95% confidence interval, and the results showed that there is a difference in the bacterial counts before the application of the herbicides (Day 0) and immediately after (day 3), and the difference is statistically significant (Z = 3.32, Z critical = 1.96, P = 0.00090). This indicates that the application of the herbicides affects the bacterial population.

show abstract

Biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of Cucurbita moschata that accumulate recombinant bacterial LinA

Cited by 15 publications

References 52 publications

Insights Into the Biodegradation of Lindane (γ-Hexachlorocyclohexane) Using a Microbial System

Insights Into the Biodegradation of Lindane (γ-Hexachlorocyclohexane) Using a Microbial System

The Use of Plants, Nanotechnology and Surfactants in Lindane Remediation: A Mini Review

Effect of Atrazine, 2,4-D Amine, Glyphosate and Paraquat Herbicides on Soil Microbial Population

Contact Info

Product

Resources

About