Die lichtstreuung von teilweise denaturierter DNS

Schurz, Von J.; Gruber, E.

doi:10.1002/macp.1969.021280108

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…Since the binding strength of DNA to aluminium-beryllium is comparable to that of DNA-ethidium to carbon, one may speculate that the appearance of the DNA strands on aluminium-berylium films reflects its conformation in solution or at the air-water interphase. This view is compatible with light scattering measurements with high polymeric salmon sperm DNA in solution (Schurz & Gruber, 1969). The fact that with the 'diffusion' method of Gordon (1973), who treats the surface of mica with aluminium chloride, and with the method of Highton & Whitfield (1974) no such 'thick and short' regions are found, is no argument against this hypothesis, since the supports used in their procedures bind nucleic acid molecules more weakly than does aluminium-beryllium.…”

Section: Discussionmentioning

confidence: 54%

Adsorption of DNA molecules to different support films

Sogo

Portmann

Kaufmann

et al. 1975

Journal of Microscopy

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SUMMARY Protein‐free adsorption of the DNA of the Escherichia coli bacteriophage T7 to carbon, collodion, aluminium‐beryllium and aluminium films was studied. It was found that the appearance of DNA strands depended greatly upon the kind of support film used. Direct adsorption of DNA to aluminium‐beryllium or aluminium films yielded specimens with ‘thin and long’ and ‘thick and short’ regions along the strand. Well extended, uncoiled and unaggregated DNA molecules were obtained only when DNA was adsorbed to carbon, collodion or mica in the presence of intercalating dyes such as ethidium bromide. Adsorption properties of the different films are well correlated with their surface charge. Aluminium‐beryllium films carry a strong positive surface charge, aluminium films a weak positive charge and carbon films a weak negative charge. It is suggested that for the preparation of specimens by spontaneous adsorption of well extended and unaggregated strands it is necessary that the DNA molecule is stiffened by a ligand such as an intercalating dye, and that the charge on the surface of the support film is opposite to the charge of the macromolecule.

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Section: Discussionmentioning

confidence: 54%

Adsorption of DNA molecules to different support films

Sogo

Portmann

Kaufmann

et al. 1975

Journal of Microscopy

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“…Covalently closed circular duplex DNA with different degrees of supercoiling shows a very complex sedimentation behavior when the counterion and ionic strength are varied (5)(6)(7)(8). Molecular dimensions have also been gauged by viscosimetry (4,9) and light scattering (10,11). Yet (wt/vol).…”

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confidence: 99%

Superstructure of linear duplex DNA.

Vollenweider¹,

Koller²,

Parello³

et al. 1976

Proc. Natl. Acad. Sci. U.S.A.

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The superstructure of a covalently closed circular DNA (of bacteriophage PM 2) was compared by electron microscopy with that of a linear duplex DNA (of bacteriophage T7) when ionic strength and benzyldimethylalkylammonium chloride concentration were varied. In parallel studies the sedimentation behavior of these DNAs was studied by analytical ultracentrifugation, but for technical reasons these had to be without benzyldimethylalkylammonium chloride. By combining the information from the two methods one has to conclude that with increasing ionic strength the linear duplex T7 DNA spontaneously forms a structure similar to that of the superhelical structure of closed circular PM 2 DNA. The superstructure is destroyed under premelting conditions and in the presence of an excess of ethidium bromide. RESULTSIn order to investigate the influence of BAC on the appearance of the DNA molecules in the electron microscope, we varied the BAC concentration between 1 X 10-5 and 5 X 10-2%

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Untersuchungen von ordnungszuständen in DNA‐Lösungen mit hilfe der lichtstreuung

Gruber

Schurz

1972

Makromol. Chem.

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0. KRATKY mit herzlichen Gliickwiinschen zum 70. Geburtstag gewidmet (Eingegangen am 9. Dezember 1971)*) ZUSAMMENFASSUNG:Aus der Winkelabhangigkeit der Lichtstreuung von salzhaltigen DNA-Losungen kaun auf das Vorliegen von Fernordnungen in der Losung geschlossen werden. Diese Ordnung bildet sich in den ruhenden Losungen im Verlauf mehrerer Stunden bis Tage aus. Sie kann durch Riihren gestort werden und verschwindet in der Nahe der Schmelztemperatur der DNA.Aus den gemessenen Extremwerten der Streufunktion laBt sich formal ein BmwscherWert von 6000 A errechnen, aber auch Periodizitaten von 2000 A bzw. 3300 A sind moglich.Zwischen 1 .lOW bis 3 .lo-* g/ml laBt sich keine eindeutige Abhangigkeit von der Konzentration feststellen, wodurch man hier ein einfaches Parallelstranggitter ausschlieBen kann. Unmittelbar nach der Filtration durch Flachedlter der Porenweite 0,s pm zeigen die DNA-Molekiile stark gestorte, knauelartige Struktur. SUMMARY:The light-scattering-function of salty DNA-solutions indicate a wide-range order in the solution. This order is developing gradually in the solution at rest within a time of several hours to several days. Stirring causes distortion of the lattice. By heating near to the melting point it disappears. From the measured extremes in the scattering function, a BRAGGvalue of 6000 A can be calculated, but periodic distances of 2000 A and 3300 A respectively are also possible. I n the concentration range from 1 .lO-4 to 3 .lO-4 g/ml no clear dependence on the concentration can be detected, so a model of equidistant parallel rods can be excluded. Immediately after filtration through a planar filter having pores of 0.5 p n in diameter, the DNA-molecules appear to be strongly distorted and are rather coillike.

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Die lichtstreuung von teilweise denaturierter DNS

Cited by 5 publications

References 10 publications

Adsorption of DNA molecules to different support films

Adsorption of DNA molecules to different support films

Superstructure of linear duplex DNA.

Untersuchungen von ordnungszuständen in DNA‐Lösungen mit hilfe der lichtstreuung

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