Die kontinuierliche Züchtung von Mikroorganismen als apparatives problem

Fiechter, Armin

doi:10.1002/bit.260070121

Cited by 18 publications

(2 citation statements)

References 70 publications

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“…Cells were grown at 30 C in a liquid synthetic medium, NL 10, previously described (20), with glucose as limiting substrate (glucose concentration So = 9.2 mg/ml). A bench-scale fermentor (Chemap AG., Mannedorf, Switzerland) served as cultivation device (6). During growth in batch as well as in continuous culture, the pH was automatically controlled at 5.5 by the addition of 2 N sodium hydroxide or 2 N phosphoric acid.…”

Section: Methodsmentioning

confidence: 99%

Enzyme Pattern and Aerobic Growth of Saccharomyces cerevisiae Under Various Degrees of Glucose Limitation

1968

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The enzyme pattern of Saccharomyces cerevisiae was followed during batch growth and in continuous culture in a synthetic medium limited for glucose under aerobic conditions. Seven enzymes were measured: succinate-cytochrome c oxidoreductase, malate dehydrogenase, nicotinamide adenine dinucleotide-linked glutamate dehydrogenase, malate synthase, isocitrate lyase, aldolase, and nicotinamide adenine dinucleotide phosphate (NADP + )-linked glutamate dehydrogenase. During fermentation of glucose and high growth rate (μ) during the first log phase in batch experiments, the first five enzymes (group I) were repressed, and aldolase and NADP + -linked glutamate dehydrogenase (group II) were derepressed. During growth on the accumulated ethyl alcohol and lower μ, the group I enzymes were preferentially formed and the other two were repressed. A sequence of derepression of the group I enzymes was found during the shift from glucose to ethyl alcohol metabolism, which can be correlated with a strong increase in the percentage of single (nonbudding) cells in the population. A correlation between the state of cells in the budding cycle and enzyme repression and derepression is suggested. In continuous culture, the enzyme pattern was shown to be related to the growth rate. The group I enzymes were repressed at high growth rates, while the group II enzymes were derepressed. Each enzyme exhibits a different dependence. The enzyme pattern is shown to depend on the rate of substrate consumption as well as on the type of metabolism and to be correlated with the budding cycle. The enzyme pattern is considered to be controlled by changes of intracellular catabolic or metabolic conditions inherent in the division cycle.

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Section: Methodsmentioning

confidence: 99%

Enzyme Pattern and Aerobic Growth of Saccharomyces cerevisiae Under Various Degrees of Glucose Limitation

1968

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“…Allerdings mußte hiezu eine geeignete Apparatur entwickelt werden, die anderwärts im Detail beschrieben worden ist (Fiechter, 1965 …”

Section: Die Untersuchungen üBer Das Verhältnis Von Gebildeter Trokkeunclassified

Wachstum und Gasstoffwechsel von <i>Saccharomyces cerevisiae </i>in kontmuierlicher Kultur

Fiechter¹,

Meyenburg²

1966

Pathobiology

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Continuous cultivation ofSaccharomyces cerevisiae. I. Growth on ethanol under steady-state conditions

Mor

Fiechter

1968

Biotechnol. Bioeng.

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Saccha romyces ce rev is iae. SummaryGrowth of Saccharomyces cereuisiae LBG H 1022 on ethanol under steady-state conditions was studied. As a cultivation device, an aerated Chemap fermentor combined with continuously working gas analyzers for oxygen and carbon dioxide was used. Dry matter, substrate concentration, yield, specific oxygen uptake, specific carbon dioxide release, and respiration quotient, as well as nitrogen, carbon, phosphorus, hydrogen, and protein content of the cells were measured in dependence on the dilution rate. Cell size distribution, as a function of the specific growth rate, was determined with the aid of a Celloscope 202. A fair agreement with the theory of continuous culture for all metabolic curves could be established. An increased turnover rate resulted from the addition of glutamic acid to the synthetic growth medium. The primary effect of this supplement could be a rise in the flow rate of the tricarboxylic acid cycle. 159

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Die kontinuierliche Züchtung von Mikroorganismen als apparatives problem

Cited by 18 publications

References 70 publications

Enzyme Pattern and Aerobic Growth of Saccharomyces cerevisiae Under Various Degrees of Glucose Limitation

Enzyme Pattern and Aerobic Growth of Saccharomyces cerevisiae Under Various Degrees of Glucose Limitation

Wachstum und Gasstoffwechsel von <i>Saccharomyces cerevisiae </i>in kontmuierlicher Kultur

Continuous cultivation ofSaccharomyces cerevisiae. I. Growth on ethanol under steady-state conditions

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