Wachstum und Gasstoffwechsel von &lt;i&gt;Saccharomyces cerevisiae &lt;/i&gt;in kontmuierlicher Kultur

Fiechter, Armin; Meyenburg, Kaspar von

doi:10.1159/000161976

Cited by 9 publications

(6 citation statements)

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“…Analytical methods. The determinations of dry weight (X), glucose (S), and ethyl alcohol concentration (A) were carried out as previously described (7). The specific glucose uptake rate Qs was calculated by the equation Qs = (S0-S)/X.D and is expressed as milligrams of glucose consumed per milligram (dry weight) per hour.…”

Section: Methodsmentioning

confidence: 99%

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Enzyme Pattern and Aerobic Growth of Saccharomyces cerevisiae Under Various Degrees of Glucose Limitation

1968

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The enzyme pattern of Saccharomyces cerevisiae was followed during batch growth and in continuous culture in a synthetic medium limited for glucose under aerobic conditions. Seven enzymes were measured: succinate-cytochrome c oxidoreductase, malate dehydrogenase, nicotinamide adenine dinucleotide-linked glutamate dehydrogenase, malate synthase, isocitrate lyase, aldolase, and nicotinamide adenine dinucleotide phosphate (NADP + )-linked glutamate dehydrogenase. During fermentation of glucose and high growth rate (μ) during the first log phase in batch experiments, the first five enzymes (group I) were repressed, and aldolase and NADP + -linked glutamate dehydrogenase (group II) were derepressed. During growth on the accumulated ethyl alcohol and lower μ, the group I enzymes were preferentially formed and the other two were repressed. A sequence of derepression of the group I enzymes was found during the shift from glucose to ethyl alcohol metabolism, which can be correlated with a strong increase in the percentage of single (nonbudding) cells in the population. A correlation between the state of cells in the budding cycle and enzyme repression and derepression is suggested. In continuous culture, the enzyme pattern was shown to be related to the growth rate. The group I enzymes were repressed at high growth rates, while the group II enzymes were derepressed. Each enzyme exhibits a different dependence. The enzyme pattern is shown to depend on the rate of substrate consumption as well as on the type of metabolism and to be correlated with the budding cycle. The enzyme pattern is considered to be controlled by changes of intracellular catabolic or metabolic conditions inherent in the division cycle.

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Section: Methodsmentioning

confidence: 99%

“…A distinct relationship was demonstrated between the degree of fermentation and oxidation and the growth rate (20). With increasing fermentative metabolism at growth rates above 0.20, a repression of the specific oxygen uptake has been observed (7,8).…”

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confidence: 99%

Enzyme Pattern and Aerobic Growth of Saccharomyces cerevisiae Under Various Degrees of Glucose Limitation

1968

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“…When grown on ethanol, the respiration rate and cytochromes are not repressed and show the normal relationship [see equation (l)] to growth rate.40 In glucose-grown cells, however, the degree of repression of respiration and cytochromes increases with glucose level and also with growth rate. 41 The results of Cowland and on Saccharomyces grown in continuous culture at various dissolved oxygen tensions indicate that the glucose repression effects may diminish at low oxygen tension.…”

Section: Response Of Growing Micro-organisms To Changes In Dissolved mentioning

confidence: 99%

Physiological effects of dissolved oxygen tension and redox potential on growing populations of micro-organisms

Harrison¹

1972

J. Appl. Chem.

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“….. Downloaded from RESULTS The repressed activity of yeast mitochondria in the presence of glucose is characterized by the decreasing rate of oxygen uptake near washout in chemostatic experiments (7,19). Degree of repression depends on the amount of glucose available for the cell (Table 1).…”

Section: -Rmentioning

confidence: 99%

“…Both the rate of oxygen uptake and the yield of the cell synthesis decrease under repressive conditions. Decrease in respiration relates more to the repression of synthesis of cell components than to the reduction of specific growth rates (7). Activity of anabolic pathways of growing cells is therefore strongly coupled with biogenesis of mitochondria.…”

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confidence: 99%

Characterization of Insoluble Protein Fractions of Mitochondria from Saccharomyces cerevisiae

et al. 1972

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Saccharomyces cerevisiae was grown in a chemostat in the presence of excess oxygen. Cells harvested from fully derepressed and strongly repressed steady states show typical promitochondria-like structures under conditions of strong repression. Insoluble membrane proteins were extracted from highly purified mitochondria and submitted to isoelectric focusing in 6% polyacrylamide gels. Some 20 protein bands were obtained from derepressed cells. The pattern was clearly different (quantitatively and possibly qualitatively) from repressed mitochondria. In contrast to ribosomal proteins, insoluble membrane protein fractions were found in the acid section (pH 4 to 6.8) of the ampholyte gels. It can be concluded that glucose repression plays a prominent role in the synthesis of the functional mitochondrial membranes.

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Wachstum und Gasstoffwechsel von <i>Saccharomyces cerevisiae </i>in kontmuierlicher Kultur

Cited by 9 publications

References 1 publication

Enzyme Pattern and Aerobic Growth of Saccharomyces cerevisiae Under Various Degrees of Glucose Limitation

Enzyme Pattern and Aerobic Growth of Saccharomyces cerevisiae Under Various Degrees of Glucose Limitation

Physiological effects of dissolved oxygen tension and redox potential on growing populations of micro-organisms

Characterization of Insoluble Protein Fractions of Mitochondria from Saccharomyces cerevisiae

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