Die durch Adrenalin bedingte Elektrolytverschiebung im Blut und Gewebe

Dresel, Kurt; Wollheim, E

doi:10.1007/bf01722700

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“…Fenn (1940) quotes the work of Sugimoto (1932), who showed that adrenaline increased the potassium content of the quadriceps muscle. Dresel & Wollheim (1924) found that in isolated loops of guinea-pig intestine adrenaline decreased the potassium content of the bath and increased that of the piece of intestine. However, their results were variable.…”

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The movement of potassium between smooth muscle and the surrounding fluid

Born¹,

Bülbring²

1956

The Journal of Physiology

101

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Previous work on ionic fluxes in excitable tissues has been mainly concerned with nerve and striated muscle; it has provided a basis for the interpretation of electrical phenomena in these tissues. From recent study it has become evident that in smooth muscle a close correlation exists between membrane potential, discharge of spike potentials and tension (Biilbring, 1955); but no information is available about ionic exchange in this tissue.The work here described was undertaken to find out whether it was possible to establish a correlation between changes in tension and the movement of potassium in smooth muscle. The results showed that during changes in tension occurring spontaneously or in response to mechanical and chemical stimuli the rates of uptake and loss of 42K were correspondingly altered. METHODSRadioactive potassium was obtained from the Atomic Energy Research Establishment, Harwell, as irradiated K2CO3 (Johnson, Matthey, 'Specpure'). The salt was dissolved in a volume of 1-0 N-HCI to neutralize the solution. The requisite amount of this radioactive solution was added to a modified Krebs's solution to give a KCI concentration of 0-35 g/l. The composition of the bathing solution used throughout was as follows (mM): 133 NaCl, 4-7 KCI, 1-38 NaH2PO4, 16-3 NaHCO3, and after equilibration with 95% 02 and 5% C02, 2-5 CaCl,2 0-105 MgC12 and 7-8 dextrose were added.The isolated taenia coli of the guinea-pig was the smooth muscle preparation used. For most experiments it was placed in a small chamber through which bathing solution flowed.The muscle chamber was made of a block of Perspex. A channel 3-2 cm long and 016 cm in diameter was drilled 0-1 cm below and parallel to the upper surface. One end of the channel was closed by a stopper with a centre hole to admit bathing fluid. The other end of the channel opened on to a Perspex lip from which the effluent could be collected. The muscle was attached at one end to a hook on the stopper, and at the other end by a thread leading out of the channel to a mechano-electronic transducer valve (RCA 5734) to record the tension. Warm water was circulated through channels drilled alongside the muscle chamber which kept the preparation at 370 C. A Geiger counter tube (G.E.C. Type GM 41 was mounted with its window downwards on top of the muscle chamber, separated from the preparation by a distance of 0-2 cm. Of this, 0-1 cm was Perspex wall, and 0-1 cm was the thickness of a lead shield. This shield had a central slot

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confidence: 99%