Die Blaufärbung des ”︁basischen Lanthanacetats„ mit Jod. Eine hochempfindliche Reaktion auf Acetat‐Ion

Krüger, Deodata; Tschirch, Erich

doi:10.1002/cber.19290621019

Cited by 34 publications

(12 citation statements)

References 14 publications

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“…Of this supernatant, 0.05 ml. gave a strongly positive lanthanum nitrate test (26,15).3 This test is positive in the presence of acetate or propionate, but negative with formate. The supernatant was then evaporated to dryness and was dissolved in 0.3 ml.…”

Section: Oxygen Uptake Of Filariae Before and After A Naerobiosismentioning

confidence: 99%

Studies on the Metabolism of the Filarial Worm, Litomosoides Carinii

Bueding

1949

Journal of Experimental Medicine

126

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During the miltiary operations in the Pacific Theatre members of the Armed Forces not infrequently were exposed to filariasis. This circumstance resulted in the initiation of a series of investigations of the chemotherapy of this disease (1-7). For a better understanding of the mechanism of action of available, and for the potential development of new, antifilarial drugs it was considered desirable to obtain information about the metabolism of filariae. Since the h u m a n forms of the filarial worm, Wuchereria bancrofli and Wucherict malayi as yet cannot be obtained for biochemical investigations, it was decided to study the metabolism of a morphologically closely related filarial worm, Litomosoides carinii (2). This organism is a common inhabitant of the pleural cavity of the wild cotton rat. MethodsThe organisms were removed from the pleural cavity of infested cotton rats and were placed in a salt medium. The medium used in the experiments described below, and referred to as a "basic filarial medium," had the following composition: 0.137 • NaC1; 0.0027 ~ KC1; 0.0003 M CaCI~; 0.001 ~ MgC12; 0.06 ~ sodium phosphate buffer (pH 7.6). After repeated washings with this medium, the filariae were blotted with No. 50 Whatman filter paper, weighed on a torsion balance, and transferred to Warburg respirometer vessels. Unless otherwise stated, 15 to 25 nag. of worms were placed into 0.8 ml. of medium in each vessel; the total volume of the vessels varied between 4 and 5 ml. The center cup contained a roll of filter paper moistened with 0

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Section: Oxygen Uptake Of Filariae Before and After A Naerobiosismentioning

confidence: 99%

Studies on the Metabolism of the Filarial Worm, Litomosoides Carinii

Bueding

1949

Journal of Experimental Medicine

126

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“…In one of the experiments the acid was redistilled, neutralized, concentrated and the lanthanum test for acetic acid [Krxiger & Tschirch, 1929;1930] carried out. The reaction was intensely positive.…”

Section: Methodsmentioning

confidence: 99%

Ethyl alcohol metabolism in animal tissues

Leloir¹,

Muñoz²

1938

Biochemical Journal

118

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BATETLL & STERN [1910] studied the oxidation of ethyl alcohol using "brei" of different organs and animals. An oxidation to acetaldehyde and acetic acid was observed, the most active organ being horse liver. Horse kidney also showed some activity. The enzymes responsible for this two-step oxidation are: for the first step (alcoholaldehyde) an alcohol dehydrogenase, and for the second (aldehyde-+ acid) either the Schardinger enzyme or a mutase. Alcohol dehydrogenase of animal origin has been studied by Reichel & Kohle [1935] but much more is known about that of yeast which has even been obtained in crystalline form [Negelein & Wulff, 1937]. It requires coenzyme I (diphosphopyridine-nucleotide) and can therefore use the yellow enzyme as a carrier. The mutase [Parnas, 1910] which might catalyse the second step is, accordingto Dixon & Lutwak-Mann [1937],different from the Schardinger enzyme and also needs coenzyme I. Many experiments have been done on the whole animal [see Le Breton, 1936] and also by liver perfusion [Lundsgaard, 1937], but the tissue slice technique has not been much used in this problem. the experimental period a loss of 3-5 % alcohol occurred, but duplicates (thermobarometers) agreed within 3 %.-Large-scale experiments. Flasks similar to those described by Krebs [1933] were filled with 22 ml. of bicarbonate-Ringer and 02+5% CO2 (pH 7.4). The initial composition of the medium was ascertained by withdrawing 10 ml. of the liquid after the gassing. The flasks were then not opened until the end of the experiment. In some cases oxygen uptake was measured in phosphate-Ringer in a parallel experiment.

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“…Sodium acetate was added as required to maintain p H a t 5. The reaction products were dialyzed against water until they reached a c o~l s t a~l t low aciditl-as measured by titration or until the washings were acetate free as indicated by the sensitive lanthanum nitrate spot test (7,13). T h e dial>.sis washings were discarded after they had been shown t o be nitrogen free.…”

Section: Degradation Of Glzrtelz Szrlplzatementioning

confidence: 99%

The Selective Degradation of Wheat Gluten

Wiseblatt¹,

Wilson²,

McConnell³

1955

Can. J. Chem.

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A method believed t o hydrolyze peptide bonds of proteins selectively a t the amino groups of serine was used t o obtain polypeptides from wheat gluten. T h e procedure involved the use of strong acid and introduced appreciable amounts of sulphur into the products possibly as sulphonic acid groups. Most of the serine appeared a t the amino terlnini of the peptides. The peptides displayed a strilcing electrophoretic homogeneity which may a t least in part be accounted for by the acquired acid groups. Osmotic pressure measurements indicated an average molecular weight near 20,000 and terminal group estimates indicate that each molecule contained several N-terminal serine residues. There appeared t o be strong association or chemical cross linking between peptide chains of the degraded gluten.

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Die Blaufärbung des ”︁basischen Lanthanacetats„ mit Jod. Eine hochempfindliche Reaktion auf Acetat‐Ion

Cited by 34 publications

References 14 publications

Studies on the Metabolism of the Filarial Worm, Litomosoides Carinii

Studies on the Metabolism of the Filarial Worm, Litomosoides Carinii

Ethyl alcohol metabolism in animal tissues

The Selective Degradation of Wheat Gluten

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