Die Amnionmembrantransplantation zur Rekonstruktion der Augenoberfläche

Kruse, F. E.; Meller, Daniel

doi:10.1007/s003470170055

Cited by 31 publications

(14 citation statements)

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“…In humans, AMT can serve mainly two different functions [4,5]. Firstly, it can be used as a patch like a con- Spoerl/Wollensak/Reber/Pillunat junctival flap to cover the ocular surface, protect it during the healing process and contribute to healing by releasing antiproteinases [6,7], thereby promoting reepithelialization and reducing inflammation [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Secondly, its basement membrane can be used as a guide rail for the corneal epithelium. In these cases the amniotic membrane is incorporated into the cornea [4,10,11] and the epithelium/basement membrane should therefore be on the outer surface. In deep stromal defects also several layers of amnion can be applied and incorporated to fill the stromal defect [11,12].…”

Section: Introductionmentioning

confidence: 99%

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Cross-Linking of Human Amniotic Membrane by Glutaraldehyde

Spoerl¹,

Wollensak²,

Reber³

et al. 2004

Ophthalmic Res

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Purpose: The transplantation of cryopreserved human amniotic membrane has been introduced recently for the reconstruction of the ocular surface. However, in some diseases the transplant usually dissolves rather quickly and early detachment may occur. Therefore, we tried to stabilize the amniotic transplant by applying glutaraldehyde for collagen cross-linking of the membrane. Methods: 18 human amnions were prepared. 4 × 4 cm pieces of amnion were treated with 0.1% glutaraldehyde solution for 30 min. Biomechanical force-elongation measurements were performed and resistance to enzymatic digestion by 0.1% collagenase solution was tested and compared to cryopreserved and untreated fresh amnion. 8 patients with various ocular surface defects were treated with cross-linked amnion and compared to 5 patients with cryopreserved amnion. Results: The force of the amnion cross-linked with glutaraldehyde at 2.5 mm elongation was increased statistically significant by 175% versus fresh amnion and 76.8% versus the cryopreserved amnion. Glutaraldehyde-treated membranes were virtually completely resistant to enzymatic digestion, while fresh and cryopreserved amnions were dissolved completely by day 7. In patients, the cross-linked membrane was preserved for up to 90 days without any signs of dissolution of the membrane and good transparency. Conclusions: Collagen cross-linking using glutaraldehyde leads to a significant increase in the biomechanical strength and enzymatic resistance of amnion, better transparency and less wrinkling. The cross-linked membrane does not dissolve for months and is well suited for the surface reconstruction of the cornea.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cross-Linking of Human Amniotic Membrane by Glutaraldehyde

Spoerl¹,

Wollensak²,

Reber³

et al. 2004

Ophthalmic Res

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“…In addition tissue growth factors and multi-potent cells of HAM can result in angiogenesis and fibroblastic activity thus, wound strength. [29181922]…”

Section: Discussionmentioning

confidence: 99%

“…[456789] However, in terms of gastrointestinal (GI) surgeries few articles have been published. To the best of our knowledge, there is only one trial assessing usage of HAM for duodenal injuries repair.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of repair in duodenal perforation with human amniotic membrane: An animal model (dog)

et al. 2014

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Background:There is a growing tendency toward application of human amniotic membrane (HAM) as a biologic substitute in various tissue injuries where a significant tissue loss is a matter of concern. In gastrointestinal injuries especially duodenal ones, some potential limitations in current surgical techniques contribute to not fully acceptable healing outcomes. Thus, this study was carried out to assess repair with HAM patch for duodenal defect in comparison with simple duodenoraphy in an animal model (dog).Materials and Methods:A total of 15 male German shepherd dogs weighing 23-27 kg were randomly divided into two groups. Group A with 10 dogs, which were a candidate for duodenal repair by amniotic membrane patch and Group B consisted of 5 dogs perform simple duodenorraphy. A precise control was made to match all conditions except surgical technique. Macroscopic and microscopic features of the healed duodenal lumen in both groups were recorded.Results:Gross evaluation revealed no difference in luminal diameter in both groups. Statistical analysis of duodenal diameter between both groups after operation also showed no significant difference (Pv = 0.789). Histological assessment indicated less inflammation with better wound healing in Group A.Conclusion:It seems that repairing duodenal wall defect with HAM would result in better histological outcomes compared with what is seen in simple duodenoraphy in animal models. However, there is no significant difference regarding surgical findings.

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“…Humane Amnionmembranen, teilweise in mehreren Lagen, wurden erstmals Ende der 90er-Jahre für die Behandlung von Hornhautulzera eingesetzt [8,11]. In weiteren Arbeiten wurde die Amnionmembran-Transplantation als effektives Verfahren zur Behandlung von Hornhautperforationen und zur Rekonstruktion der Augenoberfläche beschrieben [4,9,16]. Diese Ausgangssituation war der Grund für diese Untersuchung der Praktikabilität der Aufnähung einer mehrschichtigen Amnionmembran bei penetrierenden Hornhautulzera.…”

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