Didactic approach recounting advances and limitations in novel glutathione and cysteine detection (reduced GSH probe) with mixed coumarin, aldehyde, and phenyl–selenium chemistry

Lee, Won Chul; Mulay, Sandip V.; Shimodaira, Shingo; Abdillah, Ariq; Palma, Jaymee; Kim, Young-Sam; Yudhistira, Tesla; Churchill, David G.

doi:10.1016/bs.mie.2020.04.072

Cited by 7 publications

(2 citation statements)

References 40 publications

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“…The decontamination of chemical warfare agents (CWAs) has always been an important research subject in public safety as well as in national defense areas because of the increasing potential threats of chemical attack from terrorist organizations. − To date, great efforts have been made to develop different active materials against CWAs, including nanocrystalline metal oxides, − enzymes, − metal–organic frameworks, , transition-metal carbides, and reactive polymers. − To meet the growing demand of self-detoxifying protective clothes and coating, polymers with reactive moieties, which are capable of degradation of CWAs, have been extensively investigated because of their good processing performance, potential of being fabricated into textiles, and feasibility of sorbent modification. − It makes sense to incorporate processable polymers with self-decontamination function into protective materials, which could enhance protective performance and avoid secondary contaminations caused by CWA residue. − Moreover, polymeric decontamination materials would show completely different behaviors not only in processibility but also in mechanisms, kinetics and applicable scenarios, with the corresponding small molecule decontamination agents. Thus, a lot of research needs to be done in order to comprehensively clarify the feasibility of polymer materials as active decontamination materials as well as their decontamination behaviors.…”

Section: Introductionmentioning

confidence: 99%

Decontamination of Mustard Gas with Processable Dry Reactive Polymers via Oxidation–Chlorination

Zheng

Geng

et al. 2021

ACS Appl. Polym. Mater.

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In this study, an N-chloramide-modified reactive copolymer, chlorinated poly (4-vinylbenzenesulfonamide-co-methyl methacrylate) (PVMCl), was designed and synthesized for rapid and efficient detoxification of sulfur mustard (HD, 2-bis(2chloroethyl) sulfide) under ambient conditions. The detoxification rate of HD was up to 95% in 1 h, and HD was decomposed completely within 5 h by PVMCl both in aqueous solution and solid phase. The product characterization revealed the essential role of surface N-chloramide groups for the selective oxidation of HD. Furthermore, the self-decontamination nonwoven mat was successfully fabricated by electrospinning, which showed a satisfactory decontamination rate and long-term effect against HD.

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Section: Introductionmentioning

confidence: 99%

Decontamination of Mustard Gas with Processable Dry Reactive Polymers via Oxidation–Chlorination

Zheng

Geng

et al. 2021

ACS Appl. Polym. Mater.

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“…To unveil the ultimate biomedical mechanisms of GSH involvement in these diseases, techniques capable of intracellular GSH probing are important especially at the single-cell level due to the cellular heterogeneity. Currently, fluorescent methodology represents the predominant platform for intracellular GSH detection, − which nevertheless might involve biocompatibility (e.g., possible cytotoxicity of the nonnative indicators and light-induced toxicity from the free radicals) and bioorthogonality (e.g., perturbation of the intrinsic cellular chemistry and alteration of the contextual information). − …”

mentioning

confidence: 99%

Organic Molecular Probe Enabled Ionic Current Rectification toward Subcellular Detection of Glutathione with High Selectivity, Sensitivity, and Recyclability

Liu

Chen

et al. 2022

ACS Sens.

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Single-cell interrogation with the solid-state nanoprobes enables understanding of the linkage between cellular behavior and heterogeneity. Herein, inspired by the charge property of the organic molecular probe (OMP), a generic ionic current rectification (ICR) single-cell methodology is established, exemplified by subcellular detection of glutathione (GSH) with high selectivity, sensitivity, and recyclability. The as-developed nanosensor can transduce the subcellular OMP−GSH interaction via a sensitive ionic response, which stems from the superior specificity of OMP and its essential charge property. In addition, the nanosensor exhibits good reversibility, since the subsequent tandem reaction after the recognition can well recover the sensing surface. Given the diverse structures and tailorable charge properties of OMP, this work underpins a new and general method of OMP-based ICR single-cell analysis.

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Construction of a Highly Sensitive Thiol‐Reactive AIEgen‐Peptide Conjugate for Monitoring Protein Unfolding and Aggregation in Cells

Sabouri

Liu

Zhang

et al. 2021

Adv Healthcare Materials

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Impairment of the protein quality control network leads to the accumulation of unfolded and aggregated proteins. Direct detection of unfolded protein accumulation in the cells may provide the possibility for early diagnosis of neurodegenerative diseases. Here a new platform based on a peptide‐conjugated thiol‐reactive aggregation‐induced emission fluorogen (AIEgen), named MI‐BTD‐P (or D1), for labeling and tracking unfolded proteins in cells is reported. In vitro experiments with model proteins show that the non‐fluorescent D1 only becomes highly fluorescent when reacted with the thiol group of free cysteine (Cys) residues on unfolded proteins but not glutathione or folded proteins with buried or surface exposed Cys. When the labeled unfolded proteins form aggregates, D1 fluorescence intensity is further increased, and fluorescence lifetime is prolonged. D1 is then used to measure unfolded protein loads in cells by flow cytometry and track the aggregate formation of the D1 labeled unfolded proteins using confocal microscopy. In combination with fluorescence lifetime imaging technique, the proteome at different folding statuses can be better differentiated, demonstrating the versatility of this new platform. The rational design of D1 demonstrates the outlook of incorporation of diverse functional groups to achieve maximal sensitivity and selectivity in biological samples.

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Didactic approach recounting advances and limitations in novel glutathione and cysteine detection (reduced GSH probe) with mixed coumarin, aldehyde, and phenyl–selenium chemistry

Cited by 7 publications

References 40 publications

Decontamination of Mustard Gas with Processable Dry Reactive Polymers via Oxidation–Chlorination

Decontamination of Mustard Gas with Processable Dry Reactive Polymers via Oxidation–Chlorination

Organic Molecular Probe Enabled Ionic Current Rectification toward Subcellular Detection of Glutathione with High Selectivity, Sensitivity, and Recyclability

Construction of a Highly Sensitive Thiol‐Reactive AIEgen‐Peptide Conjugate for Monitoring Protein Unfolding and Aggregation in Cells

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