Dictyoglomus thermophilum gen. nov., sp. nov., a Chemoorganotrophic, Anaerobic, Thermophilic Bacterium

Saiki, Takahiro; Kobayashi, Y.; Kawagoe, Kunio; Beppu, Tatsuro

doi:10.1099/00207713-35-4-542a

Cited by 22 publications

(28 citation statements)

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“…A deeply branching bacterium, D. thermophilum, was observed at relatively high abundance (2% of all 16S rRNA gene sequences; Fig. 1) and with high abundance levels of proteins related to xylan degradation, in accordance with literature (32). In addition, functional insight was generated for two phylotypes affiliated with deeply branching phyla, Planctomycetes and the candidate phylum Atribacteria.…”

Section: Discussionmentioning

confidence: 49%

Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester

Hagen

Frank

Zamanzadeh

et al. 2017

Appl Environ Microbiol

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In this study, we used multiple meta-omic approaches to characterize the microbial community and the active metabolic pathways of a stable industrial biogas reactor with food waste as the dominant feedstock, operating at thermophilic temperatures (60°C) and elevated levels of free ammonia (367 mg/liter NH 3 -N). The microbial community was strongly dominated (76% of all 16S rRNA amplicon sequences) by populations closely related to the proteolytic bacterium Coprothermobacter proteolyticus. Multiple Coprothermobacter-affiliated strains were detected, introducing an additional level of complexity seldom explored in biogas studies. Genome reconstructions provided metabolic insight into the microbes that performed biomass deconstruction and fermentation, including the deeply branching phyla Dictyoglomi and Planctomycetes and the candidate phylum "Atribacteria." These biomass degraders were complemented by a synergistic network of microorganisms that convert key fermentation intermediates (fatty acids) via syntrophic interactions with hydrogenotrophic methanogens to ultimately produce methane. Interpretation of the proteomics data also suggested activity of a Methanosaeta phylotype acclimatized to high ammonia levels. In particular, we report multiple novel phylotypes proposed as syntrophic acetate oxidizers, which also exert expression of enzymes needed for both the Wood-Ljungdahl pathway and ␤-oxidation of fatty acids to acetyl coenzyme A. Such an arrangement differs from known syntrophic oxidizing bacteria and presents an interesting hypothesis for future studies. Collectively, these findings provide increased insight into active metabolic roles of uncultured phylotypes and presents new synergistic relationships, both of which may contribute to the stability of the biogas reactor.IMPORTANCE Biogas production through anaerobic digestion of organic waste provides an attractive source of renewable energy and a sustainable waste management strategy. A comprehensive understanding of the microbial community that drives anaerobic digesters is essential to ensure stable and efficient energy production. Here, we characterize the intricate microbial networks and metabolic pathways in a thermophilic biogas reactor. We discuss the impact of frequently encountered microbial populations as well as the metabolism of newly discovered novel phylotypes that seem to play distinct roles within key microbial stages of anaerobic digestion in this stable high-temperature system. In particular, we draft a metabolic scenario whereby multiple uncultured syntrophic acetate-oxidizing bacteria are capable of syntrophically oxidizing acetate as well as longer-chain fatty acids (via the ␤-oxidation and Wood-Ljundahl pathways) to hydrogen and carbon dioxide, which methanogens subsequently convert to methane.

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Section: Discussionmentioning

confidence: 49%

Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester

Hagen

Frank

Zamanzadeh

et al. 2017

Appl Environ Microbiol

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“…All members of this genus are gram-positive, except Thermoanaerobacter ethanolicus, which possesses a gram-negative cell wall (43). Members of Dictyoglomus, which are also distant phylogenetic relatives of strain SEBR 4207T and Thermoanaerobacter species, possess gram-negative cell wall (39). Interestingly, the common phenotypic element binding strain SEBR 4207T with Thermoanaerobacter and Dictyoglomus is their obligate anaerobic nature.…”

Section: Discussionmentioning

confidence: 99%

Dethiosulfovibrio peptidovorans gen. nov., sp. nov., a New Anaerobic, Slightly Halophilic, Thiosulfate-Reducing Bacterium from Corroding Offshore Oil Wells

Magot

Ravot

Campaignolle

et al. 1997

International Journal of Systematic Bacteriology

145

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A strictly anaerobic thiosulfate-reducing bacterium was isolated from a corroding offshore oil well in Congo and was designated strain SEBR 4207T. Pure culture of the strain induced a very active pitting corrosion of mild steel, with penetration rates of up to 4 mm per year. This constitutes the first experimental evidence of the involvement of thiosulfate reduction in microbial corrosion of steel. Strain SEBR 4207T cells were vibrios (3 to 5 by 1 pm), stained gram negative, and possessed lateral flagella. Spores were not detected. Optimum growth occurred in the presence of 3% NaCl at pH 7.0 and 42°C. Strain SEBR 4207T utilized peptides and amino acids, but not sugars or fatty acids. It fermented serine, histidine, and Casamino Acids, whereas arginine, glutamate, leucine, isoleucine, alanine, valine, methionine, and asparagine were only used in the presence of thiosulfate. Peptides were fermented to acetate, isobutyrate, isovalerate, 2-methylbutyrate, H,, and CO,. The addition of either thiosulfate or sulfur but not sulfate increased peptide utilization, growth rate, and biomass; during growth, H,S was produced and a concomitant decrease in H, was observed. The addition of either thiosulfate or sulfur also reversed H, inhibition. 16s rRNA sequence analysis indicates that strain SEBR 4207T is distantly related to members of the genus Thermoanuerobacter (83% similarity). Because the phenotypic and phylogenetic characteristics cannot be assigned to any described genus, strain SEBR 4207T is designated as a new species of a new genus, Dethiosulfovibrio peptidovorans gen. nov., sp. nov. Strain SEBR 4207T has been deposited in the Deutsche Sammlung von Mikroorganismen und zellkulturen GmbH (= DSM 11002).In 1989, Elf Congo experienced corrosion of the first 5 km of a 23-km main subsea pipeline that transported sour oil (i.e., H,S-containing petroleum) produced from the Emeraude oil field. The corroded segment was replaced, but it corroded again a year later. The whole line was then replaced and operated under a specifically designed biocide treatment regimen. The preliminary examination of the corroded iron showed that the breakthrough was due to bacterial pitting corrosion, with an unusually high penetration rate of about 1 cm per year. Chemical analysis of the pipeline water revealed the presence of up to 0.5 mM thiosulfate. It is likely that the thiosulfate was produced as a result of oxidation of the H,S naturally present in the oil field ecosystem by oxygen that is introduced in the pipelines during processing (10, 15, 22). The corrosion of pipelines was suspected to be due not only to sulfate but also to thiosulfate reduction by sulfate-reducing bacteria (SRB), because computer modeling had shown that thiosulfate reduction could induce the pitting corrosion of steel at higher rates than sulfate reduction (13).Since the pipeline was under a biocide treatment regimen during our microbiological investigations, the production fluids from several wellheads upstream of the line were collected and analyzed. Besides diffe...

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“…Phylogenetic analysis revealed that strains SR12 T , SR13, SR15 T and WS100 T were closely related to D. peptidovorans (DSM 11002 T ), which is a member of the subdivision containing Gram-positive bacteria with GjC contents of less than 55 mol % Saiki et al, 1985). Our tree was calculated on (Fig.…”

Section: Comparative Analysis Of 16s Rrna Sequencesmentioning

confidence: 99%

Dethiosulfovibrio russensis sp. nov., Dethosulfovibrio marinus sp. nov. and Dethosulfovibrio acidaminovorans sp. nov., novel anaerobic, thiosulfate- and sulfur-reducing bacteria isolated from 'Thiodendron' sulfur mats in different saline environments.

Surkov

Дубинина²,

Lysenko³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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The pH limits for growth were 5 5 to 8 0, with optimal growth at pH 6 5-7 0. All isolates were obligately anaerobic and slightly halophilic and grew in media containing 0 5-5 % NaCl with an optimum at 2 % NaCl. All strains were chemoorganoheterotrophic, having a fermentative type of metabolism and utilized proteins, peptides, amino acids and some organic acids, but not sugars, fatty acids or alcohols. Some organic substrates (isoleucine, valine, alanine, glutamate) were utilized only by strain SR12 T in the presence of sulfur or thiosulfate. Fermentation of citrate yielded mainly acetate, CO 2 and H 2 . Sulfur and thiosulfate were reduced to hydrogen sulfide during the fermentation of organic substances, which increased cell yields and growth rates. Sulfate, sulfite, fumarate, nitrate, Fe 2 O 3 , MnO 2 , DMSO and elemental selenium were not used as electron acceptors by these strains. The GMC contents of the DNA were 51 mol % for strains SR12 T , SR13 and SR15 T and 52 mol % for strain WS100 T . Based on morphological, physiological and phylogenetic similarities, all four isolates could be assigned to three new species of the genus Dethiosulfovibrio, named Dethiosulfovibrio russensis (type strain DSM 12538 T ), Dethiosulfovibrio marinus (type strain DSM 12537 T ) and Dethiosulfovibrio acidaminovorans (type strain DSM 12590 T ).

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Dictyoglomus thermophilum gen. nov., sp. nov., a Chemoorganotrophic, Anaerobic, Thermophilic Bacterium

Cited by 22 publications

References 0 publications

Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester

Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester

Dethiosulfovibrio peptidovorans gen. nov., sp. nov., a New Anaerobic, Slightly Halophilic, Thiosulfate-Reducing Bacterium from Corroding Offshore Oil Wells

Dethiosulfovibrio russensis sp. nov., Dethosulfovibrio marinus sp. nov. and Dethosulfovibrio acidaminovorans sp. nov., novel anaerobic, thiosulfate- and sulfur-reducing bacteria isolated from 'Thiodendron' sulfur mats in different saline environments.

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