Dicer1 Is Required for Differentiation of the Mouse Male Germline1

Maatouk, Danielle M.; Loveland, Kate; McManus, Michael T.; Moore, Karen J.; Harfe, Brian D.

doi:10.1095/biolreprod.108.067827

Cited by 199 publications

(158 citation statements)

References 42 publications

Supporting

Mentioning

151

Contrasting

Unclassified

Order By: Relevance

“…Consistent with our findings, mice with either global deletion of dicer or with oocyte-specific deletion of AGO2 display failures in oogenesis due to arrest at meiosis I [37][38][39][40]. These animals also have severely reduced number of spermatogonia, which could be due to proliferation defects and an increase in apoptosis [39,41,42]. However, it has yet to be determined in vertebrates the contribution of miRNAs in gametogenesis, since the loss of Dicer also affects the production of endogenous siRNAs, a type of small RNAs that are also important in this process [43][44][45].…”

Section: Discussionsupporting

confidence: 89%

The microRNA pathway controls germ cell proliferation and differentiation in C. elegans

et al. 2012

View full text Add to dashboard Cite

The discovery of the miRNA pathway revealed a new layer of molecular control of biological processes. To uncover new functions of this gene regulatory pathway, we undertook the characterization of the two miRNA-specific Argonaute proteins in Caenorhabditis elegans, ALG-1 and ALG-2. We first observed that the loss-of-function of alg-1 and alg-2 genes resulted in reduced progeny number. An extensive analysis of the germline of these mutants revealed a reduced mitotic region, indicating fewer proliferating germ cells. We also observed an early entry into meiosis in alg-1 and alg-2 mutant animals. We detected ALG-1 and ALG-2 protein expressions in the distal tip cell (DTC), a specialized cell located at the tip of both C. elegans gonadal arms that regulates mitosis-meiosis transition. Re-establishing the expression of alg-1 specifically in the DTC of mutant animals partially rescued the observed germline defects. Further analyses also support the implication of the miRNA pathway in gametogenesis. Interestingly, we observed that disruption of five miRNAs expressed in the DTC led to similar phenotypes. Finally, gene expression analysis of alg-1 mutant gonads suggests that the miRNA pathway is involved in the regulation of different pathways important for germline proliferation and differentiation. Collectively, our data indicate that the miRNA pathway plays a crucial role in the control of germ cell biogenesis in C. elegans.

show abstract

Section: Discussionsupporting

confidence: 89%

The microRNA pathway controls germ cell proliferation and differentiation in C. elegans

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Dead end 1 (Dnd1), an evolutionarily conserved RNA binding protein, was shown to be essential for primordial germ cell development and spermatogenesis in zebrafish and mouse by protecting certain mRNAs from miRNA-mediated repression [10][11][12][13], and was newly reported to regulate mitotic arrest and differentiation in male germ cells [14]. Additionally, Dicer, a requisite enzyme for miRNA processing, was proved to be crucial for both the male germline and Sertoli cells in spermatogenesis [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Cyclin T2: A novel miR‐15a target gene involved in early spermatogenesis

Teng

Wang

et al. 2011

FEBS Letters

View full text Add to dashboard Cite

Edited by Tamas DalmayKeywords: miR-15a Cyclin T2 Cell differentiation Spermatogenesis MicroRNA microarray a b s t r a c t MicroRNAs (miRNAs) are posttranscriptional modulators of gene expression that play important roles in various biological processes. Spermatogenesis is a highly regulated process in which diploid spermatogonia eventually differentiate into haploid spermatozoa. In this study, we identified four differentially expressed miRNAs between two premeiotic male germ cells, made predictions about their putative targets, and confirmed cyclin T2 (Ccnt2) as a direct target of miR-15a. We also report that miR-15a inhibited muscle differentiation at least in part by targeting Ccnt2, which represents a novel interaction. Subsequently, miR-15a and Ccnt2 were profiled in developing mice testes to observe their inverse correlations in the postnatal 3-week period to understand their roles in spermatogenesis.

show abstract

“…We discovered a putative DExD-box helicase (called CHAMP; cardiac helicase activated by MEF2C protein), encoded by differently sized transcripts in heart (exons [15][16][17][18][19][20][21][22][23][24][25][26] and testis (exons 1-26) (9). The testis-specific isoform has also been referred to as MOV10-like-1 (MOV10L1), on the basis of its extensive homology to Moloney leukemia virus 10 (MOV10), which was shown to interact with argonaute 1 and 2 in HeLa cells (10).…”

mentioning

confidence: 99%

MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs

Frost¹,

Hamra

Richardson

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

184

183

View full text Add to dashboard Cite

Piwi-interacting RNAs (piRNAs) comprise a broad class of small noncoding RNAs that function as an endogenous defense system against transposable elements. Here we show that the putative DExD-box helicase MOV10-like-1 (MOV10L1) is essential for silencing retrotransposons in the mouse male germline. Mov10l1 is specifically expressed in germ cells with increasing expression from gonocytes/type A spermatogonia to pachytene spermatocytes. Primary spermatocytes of Mov10l1 −/− mice show activation of LTR and LINE-1 retrotransposons, followed by cell death, causing male infertility and a complete block of spermatogenesis at early prophase of meiosis I. Despite the early expression of Mov10l1, germline stem cell maintenance appears unaffected in Mov10l1 −/− mice. MOV10L1 interacts with the Piwi proteins MILI and MIWI. MOV10L1 also interacts with heat shock 70-kDa protein 2 (HSPA2), a testis-enriched chaperone expressed in pachytene spermatocytes and also essential for male fertility. These studies reveal a crucial role of MOV10L1 in male fertility and piRNA-directed retrotransposon silencing in male germ cells and suggest that MOV10L1 functions as a key component of a safeguard mechanism for the genetic information in male germ cells of mammals.RNA helicase | Armitage | meiosis

show abstract

Dicer1 Is Required for Differentiation of the Mouse Male Germline1

Cited by 199 publications

References 42 publications

The microRNA pathway controls germ cell proliferation and differentiation in C. elegans

The microRNA pathway controls germ cell proliferation and differentiation in C. elegans

Cyclin T2: A novel miR‐15a target gene involved in early spermatogenesis

MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs

Contact Info

Product

Resources

About