Cyclin T2: A novel miR‐15a target gene involved in early spermatogenesis

Teng, Yuee; Wang, Yong; Fu, Jun; Cheng, Xiaowen; Miao, Shiying; Wang, Linfang

doi:10.1016/j.febslet.2011.06.031

Cited by 32 publications

(20 citation statements)

References 41 publications

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“…It is difficult to obtain enough highly purified primary spermatogonia cells. Therefore, we examined apoptosis in a mouse‐derived spermatogonia cell line, GC‐1 spg, which is a widely used in vitro cell model [30–32] that has the ability to differentiate into mature spermatids [16,17]. Our findings demonstrated that NLK participates in etoposide‐induced apoptosis of GC‐1 cells.…”

Section: Discussionmentioning

confidence: 91%

Nemo‐like kinase promotes etoposide‐induced apoptosis of male germ cell‐derived GC‐1 cells in vitro

et al. 2012

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a b s t r a c tSpermatogenesis is an extremely intricate process that is tightly regulated and orchestrated by a series of well-coordinated gene expression programmes. Nemo-like kinase (NLK) is an evolutionarily conserved serine/threonine kinase that functions in a wide variety of developmental events. Nevertheless, the function of NLK in spermatogenesis has not been investigated. In this study, we found that the distribution of NLK in mice exhibited a dynamic change during testicular development and gradually became concentrated in the acrosomes of elongated spermatids. NLK overexpression promoted etoposide-induced apoptosis of male germ cell-derived GC-1 cells, while knockdown of NLK by RNA interference (RNAi) attenuated etoposide-induced apoptosis. Our findings suggest that NLK plays an important role in etoposide-induced germ cell apoptosis and may be associated with spermatogenesis.

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Section: Discussionmentioning

confidence: 91%

Nemo‐like kinase promotes etoposide‐induced apoptosis of male germ cell‐derived GC‐1 cells in vitro

et al. 2012

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“…Additionally, a number of previous studies demonstrated that several miRNA species including miR-34 cluster regulate murine spermatogenesis. Mir-21, mir-34c and mir-221/222 control self-renewal of undifferentiated spermatogonia [19], Mirc1, Mirc3 and Mirlet7 regulate spermatogonial differentiation [9], [20], mir-15a and mir-184 mediate differentiation of spermatocytes [18], [19], miR-18, miR-34b, miR-34c, miR-184, miR-383, miR-449 and miR-469 mediate meiotic division of spermatocytes to spermatids [21], [23]–[25] and miR-469, miR-34c regulate differentiation of spermatid to form spermatozoa[24], [25].…”

Section: Resultsmentioning

confidence: 99%

Dysregulated microRNA Clusters in Response to Retinoic Acid and CYP26B1 Inhibitor Induced Testicular Function in Dogs

Kasimanickam

Dernell

2014

PLoS ONE

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Spermatogenesis is a multistep synchronized process. Diploid spermatogonia differentiate into haploid spermatozoa following mitosis, meiosis and spermiogenesis. Division and differentiation of male germ cells is achieved through the sequential expression of several genes. Numerous mRNAs in the differentiating germ cells undergo post-transcriptional and translational regulation. MiRNAs are powerful negative regulators of mRNA transcription, stability, and translation and recognize their mRNA targets through base-pairing. Retinoic acid (RA) signaling is essential for spermatogenesis and testicular function. Testicular RA level is critical for RA signal transduction. This study investigated the miRNAs modulation in an RA- induced testicular environment following the administration of all-trans RA (2 µM) and CYP26B1- inhibitor (1 µM) compared to control. Eighty four canine mature miRNAs were analyzed and their expression signatures were distinguished using real-time PCR based array technology. Of the miRNAs analyzed, miRNA families such as miR-200 (cfa-miR-200a, cfa-miR-200b and cfa-miR-200c), Mirlet-7 (cfa-let-7a, cfa-let-7b, cfa-let-7c, cfa-let-7g and cfa-let-7f), miR-125 (cfa-miR-125a and cfa-miR-125b), miR-146 (cfa-miR-146a and cfa-miR-146b), miR-34 (cfa-miR-34a, cfa-miR-34b and cfa-miR-34c), miR-23 (cfa-miR-23a and cfa-miR-23b), cfa-miR-184, cfa-miR-214 and cfa-miR-141 were significantly up-regulated with testicular RA intervention via administration of CYP26B1 inhibitor and all-trans-RA and species of miRNA such as cfa-miR-19a, cfa-miR-29b, cfa-miR-29c, cfa-miR-101 and cfa-miR-137 were significantly down-regulated. This study explored information regarding chromosome distribution, human orthologous sequences and the interaction of target genes of miRNA families significantly distinguished in this study using prediction algorithms. This study importantly identified dysregulated miRNA species resulting from RA-induced spermatogenesis. The present contribution serves as a useful resource for further elucidation of the regulatory role of individual miRNA in RA synchronized canine spermatogenesis.

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“…The effect can largely enhance the vascular endothelial growth factor (VEGF), which plays a major role in the angiogenesis process 38 . Similarly, Cyclin T2 (CCNT2), is involved in muscle differentiation 39 and plays an essential role in the early embryogenesis, development processes 40 , and in the early steps of spermatogenesis 41 . A pathway analysis has shown clear involvement of the miRNAs in biochemical pathways.…”

Section: Discussionmentioning

confidence: 99%

Micro-ribonucleic acids and extracellular vesicles repertoire in the spent culture media is altered in women undergoing In Vitro Fertilization

Abu‐Halima

Häusler

Backes

et al. 2017

Sci Rep

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MicroRNAs (miRNAs) are class of small RNA molecules with major impact on gene regulation. We analyzed the potential of miRNAs secreted from pre-implantation embryos into the embryonic culture media as biomarkers to predict successful pregnancy. Using microarray analysis, we profiled the miRNome of the 56 spent culture media (SCM) after embryos transfer and found a total of 621 miRNAs in the SCM. On average, we detected 163 miRNAs in SCM of samples with failed pregnancies, but only 149 SCM miRNAs of embryos leading to pregnancies. MiR-634 predicted an embryo transfer leading to a positive pregnancy with an accuracy of 71% and a sensitivity of 85%. Among the 621 miRNAs, 102 (16.4%) showed a differential expression between positive and negative outcome of pregnancy with miR-29c-3p as the most significantly differentially expressed miRNA. The number of extracellular vehicles was lower in SCM with positive outcomes (3.8 × 109/mL EVs), as compared to a negative outcome (7.35 × 109/mL EVs) possibly explaining the reduced number of miRNAs in the SCM associated with failed pregnancies. The analysis of the miRNome in the SCM of couples undergoing fertility treatment lays the ground towards development of biomarkers to predict successful pregnancy and towards understanding the role of embryonic miRNAs found in the SCM.

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Cyclin T2: A novel miR‐15a target gene involved in early spermatogenesis

Cited by 32 publications

References 41 publications

Nemo‐like kinase promotes etoposide‐induced apoptosis of male germ cell‐derived GC‐1 cells in vitro

Nemo‐like kinase promotes etoposide‐induced apoptosis of male germ cell‐derived GC‐1 cells in vitro

Dysregulated microRNA Clusters in Response to Retinoic Acid and CYP26B1 Inhibitor Induced Testicular Function in Dogs

Micro-ribonucleic acids and extracellular vesicles repertoire in the spent culture media is altered in women undergoing In Vitro Fertilization

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