Diazotised sulphanilic acid as a spectrophotometric reagent for the determination of trace amounts of indole in aqueous solution

Ahmad, Ahmad K.; Hassan, Younis I.; Bashir, Wadala A.

doi:10.1039/an9861100243

Cited by 5 publications

(4 citation statements)

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“…The azo compounds formed between diazotized sulfanilic acid and indole in aqueous solution have been early described in a spectrophotometric method (Ahmad et al 1986). This report was used as a starting point to build the present method.…”

Section: Resultsmentioning

confidence: 99%

Diazo Coupling Reaction of Catechins and Alkylresorcinols with Diazotized Sulfanilic Acid for Quantitative Purposes in Edible Sources: Method Development and Validation

et al. 2015

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Catechins and alkylresorcinols are important secondary metabolites distributed in food products, sometimes exploited in quality control and/or as markers. The current methods for their quantitative analysis have some disadvantages such as cost, time consuming, and even insensitive or non-selective. Therefore, suitable, efficient methods are still required. As part of our research on new and rapid methods of analyses in plant and food products, a spectrophotometric method to quantify alkylresorcinols and catechins in plant products was developed and fully validated. The colored product by the diazo coupling between olivetol and catechin with diazotized sulfanilic acid was employed to develop the method. The effect of acid and diazonium salt concentration and the reaction time was analyzed. The method was linear in 0.8-8.3 and 0.6-10.2 μg/mL ranges to olivetol and catechin, respectively. Limit of detection and limit of quantification for olivetol and catechin were 0.253/0.768 and 0.106/0.321 μg/ mL, respectively. Precision at intra-day and inter-day levels (relative standard deviation (RSD) 1.3-9.7 %) and accuracy (99.0-104.9 %) were also demonstrated. Additionally, the application of the method to plant samples containing alkylresorcinol or catechins was subsequently evaluated, being comparable with conventionally employed methods. Thus, the method demonstrated to be fast, easy, inexpensive, and reliable for quantifying these kinds of metabolites in food products.

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Section: Resultsmentioning

confidence: 99%

Diazo Coupling Reaction of Catechins and Alkylresorcinols with Diazotized Sulfanilic Acid for Quantitative Purposes in Edible Sources: Method Development and Validation

et al. 2015

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“…is added, the mixture is left in ice bath at 0 -5C and 0.207g sodium nitrite is added and stirred vigorously and then the volume completed to 100 ml in a volumetric flask using cooled distilled water, and is stored in refrigerator. This solution is prepared freshly each day [17].…”

Section: Apparatusmentioning

confidence: 99%

Spectrophotometric Determination of Oxymetazoline Hydrochloride in Pure and Pharmaceutical Preparations Using Diazo-coupling Reaction

Al-Ghabsha¹,

Obedagha²,

Zakaria³

2019

J.Edu.Sci.

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A ccurate and simple spectrophotometric method has been suggested for the determination of oxymetazoline hydrochloride (OMCl) in aqueous solution is developed. The present process included coupling of OMCl with diazotized sulphanilic acid reagent in the presence of sodium hydroxide. The orange colored azo dye formed very stable and soluble in water gives maximum absorption at 496 nm. The lineratey is obeyed over the range 20-400 g / 25 ml (0.8-16µg.ml-1) the molar absorptivity is equate to 2.30  10 4 l.mol-1 cm-1. The proposed method has been used to the determination of OMCl in two formulations with satisfactories results.

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“…32 The azo compounds formed between diazotized sulfanilic acid and indole in aqueous solution have been early described in a spectrophotometric method. 35,36 In our work, the development of the reaction occurs in two steps: firstly, the reaction of sulfamethoxazole (SMZ) with sodium nitrite occurs in an acid medium (HCl) producing the diazo compound. Secondly, the diazo compound in alkaline medium coupled with the BPA produced a compound that was monitored at 445 nm.…”

Section: Reaction Mechanismmentioning

confidence: 99%

Development of a Novel Spectrophotometric Method Based on Diazotization- Coupling Reaction for Determination of Bisphenol A

Xu¹,

Wu²,

Duan³

et al. 2016

J. Braz. Chem. Soc.

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Bisphenol A (BPA) is an industrial material which can lead to endocrine disorders. In this work, a simple and sensitive spectrophotometric method for determination of BPA using diazotizationcoupling reaction was presented. To achieve the best performance of the developed method, the experimental parameters were optimized in terms of reagent concentration, reaction time, pH, and temperature. Under the optimal conditions, the absorbance increased linearly with the increasing concentration of BPA in the range of 0.16-10.4 μg mL-1 with the correlation coefficient of 0.9991 and the detection limit of 0.05 μg mL-1. Results achieved with the spectrophotometric method were compared with those obtained using high performance liquid chromatography (HPLC) method. The proposed method was successfully applied to determine BPA in milk and water bottle samples and satisfactory results were obtained, which make it very suitable for routine analysis of BPA in quality control laboratories.

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Diazotised sulphanilic acid as a spectrophotometric reagent for the determination of trace amounts of indole in aqueous solution

Cited by 5 publications

References 0 publications

Diazo Coupling Reaction of Catechins and Alkylresorcinols with Diazotized Sulfanilic Acid for Quantitative Purposes in Edible Sources: Method Development and Validation

Diazo Coupling Reaction of Catechins and Alkylresorcinols with Diazotized Sulfanilic Acid for Quantitative Purposes in Edible Sources: Method Development and Validation

Spectrophotometric Determination of Oxymetazoline Hydrochloride in Pure and Pharmaceutical Preparations Using Diazo-coupling Reaction

Development of a Novel Spectrophotometric Method Based on Diazotization- Coupling Reaction for Determination of Bisphenol A

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