Diazinon effect on the activities of brain enzymes fromOphiocephalus (Channa) punctatus

Sastry, K.V.; Sharma, Kamana

doi:10.1007/bf01608117

Cited by 31 publications

(14 citation statements)

References 16 publications

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“…Significance: *p < 0.05; **p < 0.01 Sastry and Sharma (1980) report decreased lactate dehydrogenase activity in the brain of Channa punctata following an action of diazinon lasting 96 h. According to our results, the assumed decrease in lactate dehydrogenase activity in the brain was mirrored even in the significantly decreased activity of this enzyme in the blood plasma of our carp exposed to diazinon (p < 0.05). On the other hand, the activities alkaline and acid phosphatases in blood plasma were almost identical in the control and experimental groups of carp.…”

Section: -Control Group; E -Experimental Groupsupporting

confidence: 63%

“…The major biochemical response to the effect of diazinon in fishes is the inhibition of a number of enzymes, above all acetylcholinesterase (Goodman et al 1979;Sastry and Sharma 1980;Ansari et al 1987;Hamm et al 1998). Also observed have been changes in carbohydrate metabolism in the eel, Anguilla anguilla, during short-term exposure to diazinon (Ceron et al 1997).…”

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confidence: 99%

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Effect of Diazinon on Blood Plasma Biochemistry in Carp (Cyprinus carpio L.)

Lusková¹,

Svoboda²,

Kolářová³

2002

Acta Vet. Brno

106

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Lusková V., M. Svoboda, J. Koláfiová: The Effect of Diazinon on Blood Plasma Biochemistry in Carp (Cyprinus carpio L.). Acta Vet. Brno 2002, 71: 117-123.The aim of this study was to evaluate the effect of diazinon [0,0-diethyl-0-(2-isopropyl-6-methylpyrimidin-4yl) phosphorothioate] on common carp (Cyprinus carpio L.). The effect was assessed by comparing the biochemical blood plasma profiles of a control group and a group exposed to the effect of the pesticide Basudin 600 EW (containing 600 g.l -1 diazinon as the toxic substance). The activities of selected enzymes, metabolite concentrations and electrolytes were measured in 15 specimens of controls, K 1-2, and in 16 specimens, K 1-2 , exposed for 96 h to the effects of Basudin 600 EW at a concentration of 32.5 mg.l -1 . Compared with the control group, a significant decrease of cholinesterase (p < 0.01) and lactate dehydrogenase (p < 0.05) was ascertained in the experimental group. The values of alanine and aspartate aminotransferases, creatine kinase, alkaline and acide phosphatases were comparable in the experimental and control groups. The total protein and lactate concentration were significantly lower (p < 0.05) in the experimental group, compared with the control group. On the contrary, glucose concentration in the plasma of the experimental group was significantly higher (p < 0.01) than in that of the control group. The experimental group showed a significantly higher (p < 0.05) concentration of plasmatic natrium and potassium, and a significantly lower (p < 0.05) concentration of plasmatic calcium and phosphorus, compared with those in the control group. The above results of examinations of the biochemical blood plasma profile indicate a marked neurotoxic effect of diazinon in fishes.

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Section: -Control Group; E -Experimental Groupsupporting

confidence: 63%

mentioning

confidence: 99%

Effect of Diazinon on Blood Plasma Biochemistry in Carp (Cyprinus carpio L.)

Lusková¹,

Svoboda²,

Kolářová³

2002

Acta Vet. Brno

106

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“…There is an inhibition of a whole series of enzymes and mainly of acetylcholinesterase (Goodman et al 1979;Sastry and Sharma 1980;Ansari et al 1987;Hamm et al 1998).…”

mentioning

confidence: 99%

The Effect of Diazinon on Haematological Indices of Common Carp (Cyprinus carpio L.)

Svoboda¹,

Lusková²,

Drastichová³

et al. 2001

Acta Vet. Brno

139

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The goal was to assess an effect of diazinon [0,0-diethyl 0-(2-isopropyl-6-methylpyrimidin-4yl) phosphorothioate] on common carp (Cyprinus carpio L.). The effect was assessed based on results of acute toxicity tests and on a comparison of results of haematological examination of a control and an experimental group exposed to Basudin 600 EW pesticide preparation (active substance 600 g.l -1 of diazinon). The acute toxicity test lasting 96 h was performed semistatically on common carp juveniles. Examination of erythrocyte and leukocyte profile was performed on 15 control and 25 experimental specimens of one-to-two-year-old common carp after 96 h of exposure to Basudin 600 EW in concentration of 32.5 mg . l -1 . The 96hLC50 value of Basudin 600 EW for common carp juveniles was 26.7 mg . l -1 . The experimental group of one-to two-year-old common carp showed significantly lower values (p < 0.01) of erythrocyte count (RBC), haemoglobin content (Hb) and haematocrit (PCV) compared to the control group. Values of MCV, MCH and MCHC were comparable in both groups during the study. In contrary, there was a significant decrease in leukocyte count (Leuko) (p < 0.01), as well as in both the relative and absolute lymphocyte count (p < 0.01) and a significant increase in both the relative and absolute count of developmental forms of neutrophile granulocytes: myelocytes (p < 0.01) and metamyelocytes (p < 0.05) in the experimental group. Relative and absolute count of monocytes and both the band-and segmented neutrophile granulocytes was comparable in both groups during the study. The diazinon-based Basudin 600 EW pesticide preparation was classified among harmful substances for fish. Changes in values of both the erythrocyte and leukocyte profile after exposure to diazinon-based preparation may be refered to disruption of haematopoiesis as well as to a decrease on non-specific immunity of the fish.Organophosphorous pesticide, acute toxicity, erythrocyte profile, leukocyte profile

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“…The enzyme shows an increasing activity during a strenuous muscle exercise (Rajamanickam and Muthuswamy, 2008). Lactate dehydrogenase is released from the liver after its cellular damage (Ceron et al, 1997) this may be the reason for the significance decrease in the liver lactate dehydrogenase; thus suggesting that its activity is a sensitive index to measure influence of external factors as supported by the findings of Sastry and Sharma (1980) who reported decreased in lactate dehydrogenase activity in the brain of Channa punctatus exposed to diazinon. Palikova et al (2004) also reported significant decrease in lactate dehydrogenase activity of Carp (Cyprinus carpio L.) with long term exposure to cyanobacteria extract during the early life stages.…”

Section: Resultsmentioning

confidence: 88%

Sublethal Effects of Chromium on Enzymatic Activities of the African Catfish: Clarias Gariepinus (Burchell, 1822)

2012

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The effect of sublethal concentration (0.00, 2.00, 4.00 and 8.00 mg/l) of chromium was determined on the activities of some enzymes (creatine-kinase, lactate dehydrogenase, cholinesterase, gamma glutamyltransferase) in the plasma, liver and kidney of the exposed juvenile African Catfish Clarias gariepinus (length 23.00±0.86 cm and weight 96.97±5.31 g respectively) after the 7 days exposure period. The activity of lactate dehydrogenase was significantly (p<0.05) decrease in plasma, liver and kidney while cholinesterase showed an insignificant decrease and increase (p>0.05) in plasma and liver and a significant decrease (p<0.05) in kidney after the 7 days exposure period. The activities of gamma glutamyltransferase and creatine-kinase were insignificantly decreased (p>0.05) in plasma, liver and kidney except liver creatine kinase that was significantly decreased. Generally, the activities of the determined enzymes were most significant in the liver than in plasma and kidney. Therefore, sublethal concentrations of chromium have some deleterious effect on the basic activities of enzymes of the plasma, liver and kidney of Clarias gariepinus as revealed in this investigation.

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Diazinon effect on the activities of brain enzymes fromOphiocephalus (Channa) punctatus

Cited by 31 publications

References 16 publications

Effect of Diazinon on Blood Plasma Biochemistry in Carp (Cyprinus carpio L.)

Effect of Diazinon on Blood Plasma Biochemistry in Carp (Cyprinus carpio L.)

The Effect of Diazinon on Haematological Indices of Common Carp (Cyprinus carpio L.)

Sublethal Effects of Chromium on Enzymatic Activities of the African Catfish: Clarias Gariepinus (Burchell, 1822)

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