Effect of Diazinon on Blood Plasma Biochemistry in Carp (Cyprinus carpio L.)

Abstract

Lusková V., M. Svoboda, J. Koláfiová: The Effect of Diazinon on Blood Plasma Biochemistry in Carp (Cyprinus carpio L.). Acta Vet. Brno 2002, 71: 117-123.The aim of this study was to evaluate the effect of diazinon [0,0-diethyl-0-(2-isopropyl-6-methylpyrimidin-4yl) phosphorothioate] on common carp (Cyprinus carpio L.). The effect was assessed by comparing the biochemical blood plasma profiles of a control group and a group exposed to the effect of the pesticide Basudin 600 EW (containing 600 g.l -1 diazinon as the toxic substance). The activities of selected enzymes, metabolite concentrations and electrolytes were measured in 15 specimens of controls, K 1-2, and in 16 specimens, K 1-2 , exposed for 96 h to the effects of Basudin 600 EW at a concentration of 32.5 mg.l -1 . Compared with the control group, a significant decrease of cholinesterase (p < 0.01) and lactate dehydrogenase (p < 0.05) was ascertained in the experimental group. The values of alanine and aspartate aminotransferases, creatine kinase, alkaline and acide phosphatases were comparable in the experimental and control groups. The total protein and lactate concentration were significantly lower (p < 0.05) in the experimental group, compared with the control group. On the contrary, glucose concentration in the plasma of the experimental group was significantly higher (p < 0.01) than in that of the control group. The experimental group showed a significantly higher (p < 0.05) concentration of plasmatic natrium and potassium, and a significantly lower (p < 0.05) concentration of plasmatic calcium and phosphorus, compared with those in the control group. The above results of examinations of the biochemical blood plasma profile indicate a marked neurotoxic effect of diazinon in fishes.

Modulation of Biochemical and Haematological Indices of Silver Carp (Hypophthalmichthys molitrix Val.) Exposed to Toxic Cyanobacterial Water Bloom

“…Kopp et al (2005) showed that in silver carp exposed to toxic cyanobacterial population, serum activities of ALP significantly decreased, and activities of CHE significantly increased. The ALP activities of carp did not change in water that contained the pesticide diazinon (Lusková et al 2002). Our results of alkaline phosphatase and creatinine activities support the assumption that the liver and muscle tissues of experimental fish were not markedly affected.…”

“…In contrast, Lusková et al (2002) reported a decrease of plasma lactate concentration in carp kept in water containing the pesticide diazinon. In our experiment, a significant decrease of lactate concentration in the blood plasma of experimental fish compared to control may indicate a higher metabolic rate of lactate in the hepatopancreas and/or it might be a result of stress caused by cyanobacteria.…”

“…Bury et al (1996) and Ernst et al (2006) observed slightly increased glucose concentrations in fish (brown trout and whitefish, respectively) exposed to cyanobacteria, but changes were not significant. An increase in glucose is a general response of fish to acute pollutant effects and stress (Lusková et al 2002;Svobodová et al 2006). Consequently, stress as indicated by plasma glucose changes does not appear to be associated with liver pathology.…”

“…The changes of haematological and biochemical indices in fish could be caused by chemical factors (Kopp and Heteša 2000;Lusková et al 2002;Pepeljnjak et al 2003), age, health and body condition (Svetina et al 2002), nutrition (Serpunin 1995) or stress (Dobšíková et al 2006).…”

Effect of Single Superphosphate Fertilizer on Survival and Respiratory Dynamics of Nile Tilapia, <I>Oreochromis Niloticus</I> (Actinopterygii: Perciformes: Cichlidae)

“…In addition, reduction in the activity levels of respiratory enzymes such as LDH and ADH and the depletion in the level of consumed oxygen could all have contributed to the observed behaviour and eventual mortality. This phenomenon was supported by the investigations of Lusková et al (2002) and Das et al (2004). Ceron et al (1997) had earlier reported a reduction in lactate concentration when fish were exposed to Diazinon at acute concentrations.…”