2005
DOI: 10.1590/s0100-40422005000500007
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Diazepam e nordiazepam em plasma: métodos de extração líquido-líquido e em fase sólida no pré-tratamento de amostras para análise cromatográfica em fase líquida

Abstract: Recebido em 16/4/04; aceito em 10/1/05; publicado na web em 13/4/05 DIAZEPAM AND NORDIAZEPAM IN PLASMA: LIQUID-LIQUID AND SOLID PHASE EXTRACTION IN SAMPLE PRE-TREATMENT FOR HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS. Analysis of diazepam (DZP) and its active metabolite nordiazepam (NDZP) in plasma is commonly performed in clinical medicine to ensure proper therapeutic effects while minimizing the incidence of toxicity. This study aimed to optimize analytical parameters and compare two pre-treatment techni… Show more

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Cited by 6 publications
(3 citation statements)
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“…The approach used to decide which compound to use for this purpose was a satisfactory electrochemical response; flunitrazepam was chosen as internal standard. This compound was previously used in a study conducted by Freire et al [26], when diazepam and nordiazepam were determined in plasma by a HPLC-UV method.…”
Section: Methods Validationmentioning
confidence: 99%
See 1 more Smart Citation
“…The approach used to decide which compound to use for this purpose was a satisfactory electrochemical response; flunitrazepam was chosen as internal standard. This compound was previously used in a study conducted by Freire et al [26], when diazepam and nordiazepam were determined in plasma by a HPLC-UV method.…”
Section: Methods Validationmentioning
confidence: 99%
“…The accuracy was evaluated through analyses of pharmaceutical formulations, by the proposed HPLC-BDD method and by an HPLC-DAD method, a ;previously described procedure [26], with some modifications. For HPLC-DAD the chromatographic separation was achieved using a Waters XTerra RP18 analytical column (250 Â 4.6 mm, 5 mm), the mobile phase employed was sodium phosphate (pH 3.5; 0.10 mol L À1 )-acetonitrile (65 : 35, v/v), isocratic elution with a flow rate of 1.2 mL min À1 and detection was performed at 228 nm.…”
mentioning
confidence: 99%
“…DZP and NOR concentrations were determined as previously described (Freire et al, 2005). Aliquots of 500 L rat plasma were added to 10 L of the IS (100 g/mL nitrazepam) and 2 mL of sodium hydrogen phosphate pH 9.0, then extracted with 5 mL nhexane: ethanol (90:10, vol/vol) for 3 min in a vortex mixer.…”
Section: Dzp Analysismentioning
confidence: 99%