Dialysis-induced serum factors inhibit adherence of monocytes and granulocytes to adult human endothelial cells

Thylén, Pia; Fernvik, Eva; Hægerstrand, Anders; Lundahl, Joachim; Jacobson, Stefan H.

doi:10.1016/s0272-6386(97)90011-1

Cited by 22 publications

(19 citation statements)

References 33 publications

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“…In vitro activation with fMLP induced a significant increase in the expression of CD11b on monocytes and neutrophils in the peripheral circulation and at the sites of interstitial inflammation, both in patients on highflux hemodialysis/hemodiafiltration and healthy subjects. The preserved capacity of both monocytes and neutrophils to express CD11b at the sites of interstitial inflammation in patients on high-flux hemodialysis/hemodiafiltration, as shown by our findings, may have important biological consequences in terms of an adequate performance of leukocyte functions in which the CD11b molecule plays a key role (Thylen et al 1997;Moshfegh et al 2002). Extravasated neutrophils and monocytes from patients on high-flux hemodialysis/hemodiafiltration showed a maintained response to fMLP as a second inflammatory stimulus after extravasation.…”

Section: Cd11bsupporting

confidence: 62%

“…factor D) (Clark et al 1999;Schindler et al 2006). Dialysis can reduce leukocyte-endothelial interactions and impair transmigration (Thylen et al 1997). In patients on hemodialysis with cuprophane or polysulfone membranes, a significantly higher serum level of MCP-1 is seen compared with healthy subjects both before and after the hemodialysis session, independent of the membrane used (Jacobson et al 2000;Thylen et al 2000).…”

Section: Patients On Hemodialysismentioning

confidence: 99%

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Leukocyte Function in High-Flux Hemodialysis

Olsson¹

2011

Progress in Hemodialysis - From Emergent Biotechnology to Clinical Practice

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Section: Cd11bsupporting

confidence: 62%

Section: Patients On Hemodialysismentioning

confidence: 99%

Leukocyte Function in High-Flux Hemodialysis

Olsson¹

2011

Progress in Hemodialysis - From Emergent Biotechnology to Clinical Practice

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“…In fact, the quantitative expression of adhesion molecules on inflammatory cells does not always mirror the functional state of the cell [1]. Previous studies performed on endothelial cells in culture and in experimental animals suggest that dialysis with cuprophan membranes results in diminished leukocyte rolling and impaired endothelial adhesion [9, 11, 12, 13]. However, whether this also results in impaired transmigration properties is not known.…”

Section: Introductionmentioning

confidence: 99%

Impact of Hemodialysis Membrane and Permeability on Neutrophil Transmigration in vitro

Moshfegh¹,

Jacobson²,

Halldén³

et al. 2002

Nephron

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Background/Aim: The impact of dialysis membrane permeability on neutrophil transmigration properties in vitro was examined in the present study. This issue has not been fully scrutinized before. Methods: We studied the capacity of neutrophils collected from a group of dialysis patients randomly treated with cuprophan, low- or high-flux polysulfone, to transmigrate in vitro through a membrane covered with fibronectin (a main constituent of the endothelial basement membrane). The hemodialysis-induced quantitative changes in expression of adhesion molecules were examined in parallel. Results: At the end of dialysis, neutrophils collected from patients treated with high-flux polysulfone dialyzers had a significantly higher transmigration index than neutrophils from patients treated with low-flux polysulfone membrane (p < 0.01) or cuprophan membrane (p < 0.01), and approached the level of transmigration observed in neutrophils collected from healthy controls. In the groups treated with low-flux polysulfone and cuprophan dialyzers, the transmigration capacity was significantly lower (p < 0.02) compared to neutrophils from healthy subjects. We also noted that differences between low- and high-flux polysulfone dialysis, in the context of transmigration properties, were not mirrored by changes in adhesion phenotype, which strengthens the view that there is no strict relationship between these two features. Conclusion: The study demonstrates that high-flux polysulfone dialysis, as opposed to low-flux polysulfone and cuprophan treatment, improves the transmigration properties of circulating neutrophils, despite similar effects on adhesion molecule phenotypes. A plausible mechanism is that potentially toxic middle range molecules that inhibit neutrophil migration are more efficiently eliminated during high-flux polysulfone dialysis, but this explanation requires further support.

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“…Patients undergoing maintenance hemodialysis exhibit a disruption in the sequence of events leading to adhesion and changes in adhesive and cohesive (aggregation) properties of leukocytes [16, 22]. The purpose of the present study was to assess whether activated platelets and leukocytes from patients subjected to the previously characterized dialysis membranes were capable of activating ECs and or promoting EC adhesiveness.…”

Section: Discussionmentioning

confidence: 99%

“…Activation of cells with subsequent changes in adhesion molecule expression takes place in the extracorporeal circuit, far away from the respective EC ligand [16, 17]. In patients on hemodialysis, the sequences of events required for adhesion molecule-mediated adherence of monocytes and granulocytes to ECs are disrupted.…”

Section: Introductionmentioning

confidence: 99%

Effect of Two Different Dialysis Membranes on Leukocyte Adhesion and Aggregation

Hernández¹,

Palomo²,

Fusté³

et al. 2007

Nephron Clin Pract

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Aims: We evaluated modifications in formation of heterotypic platelet-leukocyte aggregation induced by dialysis through cellulosic or synthetic membranes and evaluated the effects of such procedures promoting adhesive interactions between leukocytes and normal endothelial cells (ECs). Methods: Samples were obtained from arterial and venous lines at baseline, after 15 and 120 min of hemodialysis. Heterotypic aggregation was assessed using flow-cytometric techniques. Experiments to determine leukocyte adhesion to ECs were performed in parallel plate perfusion chambers at 450 s^–1. Results: Patients dialyzed with a cellulosic membrane showed a significantly higher baseline granulocyte heterotypic aggregation (median 22.5%, range 8.6–32%) versus healthy subjects (median 10%, range 3.2–14.6%; p < 0.05). Granulocyte heterotypic aggregation values remained increased throughout the hemodialysis session not only in the arterial line (median 18 and 24.5%, range 7–30 and 8.7–36% at 15 and 2 h, respectively) but also in the venous line (median 20 and 25%, range 8.6–32 and 11.5–35% at 15 min and 2 h, respectively). Basal lymphocytes heterotypic aggregation values observed in uremic patients were 6% (0.1–7.1%) versus 1.0% (0.5–2.8%) in the control group (p < 0.05). The increase remained during the hemodialysis session both in the arterial line (median 5 and 4%, range 0.2–14 and 0.5–7.1 % at 15 min and 2 h, respectively) and in the venous line (median 7 and 7%, range 1.4–14 and 0.5–10.6% at 15 min and 2 h). In contrast, patients dialyzed with a synthetic membrane showed a decreased basal granulocyte heterotypic aggregation compared to healthy subjects (median 3.5 vs. 10%, range 2.8–7 vs. 3.2–14.6%, respectively; p < 0.05). For lymphocytes, basal heterotypic aggregation values were 0.2% (range 0.1–0.5%) in dialyzed patients vs. 0.98% (range 0.5–2.8%) in healthy subjects (p < 0.05), without changes throughout the dialysis session. Changes in leukocyte adhesion during hemodialysis did not reach statistical significance with either hemodialysis membrane. Our studies confirm a differential activation of platelets and leukocytes depending on the nature of the dialysis membranes. However, activation of circulating cellular elements by hemodialysis procedures did not enhance cross-talk interactions between leukocytes and unaltered ECs.

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Dialysis-induced serum factors inhibit adherence of monocytes and granulocytes to adult human endothelial cells

Cited by 22 publications

References 33 publications

Leukocyte Function in High-Flux Hemodialysis

Leukocyte Function in High-Flux Hemodialysis

Impact of Hemodialysis Membrane and Permeability on Neutrophil Transmigration in vitro

Effect of Two Different Dialysis Membranes on Leukocyte Adhesion and Aggregation

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