Diagnóstico serológico de las infecciones por Mycoplasma pneumoniae

Andreu, Lurdes Matas; Abós, Sònia Molinos; Rivas, Gema Fernández; Soler, Victoria González; Ruiz, Vicente Ausina

doi:10.1157/13094274

Cited by 14 publications

(6 citation statements)

References 12 publications

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“…Mycoplasmas are the smallest free-living prokaryotes known, having an extremely small genome size of 580-2200 kilobase pair. Mycoplasmas are classified in the family Mycoplasmataceae and order Mycoplasmatales 9 10 13 14 . Six of the 16 species of human mycoplasma cause diseases, the most important and the most predominant pathogen is M. pneumoniae 14 .…”

Section: Biology Of Mycoplasma Pneumoniae and Pathmentioning

confidence: 99%

“…Six of the 16 species of human mycoplasma cause diseases, the most important and the most predominant pathogen is M. pneumoniae 14 . Mycoplasmas cannot be detected by light microscopy due to their small size, and also, visible turbidity is not produced in liquid growth medium due to their small cellular mass 9 10 13 . The absence of a cell wall barrier in mycoplasmas is unique among the prokaryotes, and so, these organisms are not sensitive to cell wall antimicrobial agents such as beta-lactams, are not stained by Gram staining, are very susceptible to effect of drying and also influence their pleomorphic appearance 15 .…”

Section: Biology Of Mycoplasma Pneumoniae and Pathmentioning

confidence: 99%

“…Several tests such as immunoblotting assay, immunofluorescence assay (IFA), counter-immunoelectrophoresis assay and antigen-capture enzyme immunoassay (EIA) can be used. The limits of detection are in the range of 10 3 -10 5 colony forming units/ml with a limited sensitivity and specificity 9 13 .…”

Section: Diagnosismentioning

confidence: 99%

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Mycoplasma pneumoniae: A significant but underrated pathogen in paediatric community-acquired lower respiratory tract infections

Kumar

2018

Indian J Med Res

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Lower respiratory tract infections are considered a common cause responsible for morbidity and mortality among children, and Mycoplasma pneumoniae is identified to be responsible for up to 40 per cent of community-acquired pneumonia in children greater than five years of age. Extrapulmonary manifestations have been reported either due to spread of infection or autoimmune mechanisms. Infection by M. pneumoniae has high incidence and clinical importance but is still an underrated disease. Most widely used serologic methods are enzyme immunoassays for detection of immunoglobulin M (IgM), IgG and IgA antibodies to M. pneumoniae, though other methods such as particle agglutination assays and immunofluorescence methods are also used. Detection of M. pneumoniae by nucleic acid amplification techniques provides fast, sensitive and specific results. Utilization of polymerase chain reaction (PCR) has improved the diagnosis of M. pneumoniae infections. Besides PCR, other alternative amplification techniques include (i) nucleic acid sequence-based amplification, (ii) Qβ replicase amplification, (iii) strand displacement amplification, (iv) transcription-mediated amplification, and (v) ligase chain reaction. Macrolides are used as the first-line treatment in childhood for M. pneumoniae infections; however, emergence of macrolide-resistant M. pneumoniae is a cause of concern. Development of a safe vaccine is important that gives protective immunity and would be a major step in reducing M. pneumoniae infections.

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Section: Biology Of Mycoplasma Pneumoniae and Pathmentioning

confidence: 99%

Section: Biology Of Mycoplasma Pneumoniae and Pathmentioning

confidence: 99%

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Mycoplasma pneumoniae: A significant but underrated pathogen in paediatric community-acquired lower respiratory tract infections

Kumar

2018

Indian J Med Res

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“…Previously, diagnosis usually relied on serology. [ 17 ] However, serology is not reliable in specificity, needs paired sera, and is usually positive at about 7 days after the onset of disease. [ 18 ] In contrast, molecular tests such as real-time PCR on throat swab and other respiratory secretions may provide an early diagnosis of M. pneumoniae infection and could be used as an useful diagnostic technology.…”

Section: Discussionmentioning

confidence: 99%

Clinical spectrum and diagnostic yields of Mycoplasma pneumoniae as a causative agent of community-acquired pneumonia

et al. 2018

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INTRODUCTION:Infection with Mycoplasma pneumoniae (M. pneumonia) occurs worldwide which accounts for 15%–20% of cases of community-acquired pneumonia and indistinguishable clinically from other infectious causes of pneumonia.AIM:The aim of this study was to evaluate the real-time polymerase chain reaction (PCR) and to correlate it with other diagnostic methods such as culture, serology (ELISA), and conventional PCR along with the clinical signs and symptoms produced by M. pneumonia.MATERIALS AND METHODS:A total of 130 patients of all age groups presenting with clinical features of lower respiratory tract infections were enrolled over a period of 1 year and 2 months in a tertiary care hospital in Delhi. M. pneumoniae in throat swab samples was detected by real-time PCR, compared with culture, serology, conventional PCR, and clinical signs and symptoms. Univariate analyses were conducted to determine the association of M. pneumoniae infection among different categories of patients.RESULTS:Out of a total of 130 patients, 18 patients (14%) were positive for M. pneumoniae by any test; culture was positive in nine patients (50%), serology (IgM) in eight patients (44.4%), PCR in five patients (27.7%), and real-time PCR was positive in six patients (33.3%). Clinical signs and symptoms were higher in incidence in M. pneumoniae-positive patients. Age-matched healthy controls (30) were included in the study, and all were negative for any diagnostic test performed (P = 0.026).CONCLUSION:It was concluded that combination of M. pneumoniae-specific testing modalities is required for the diagnosis of this etiological agent rather than a single diagnostic method.

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“…Mycoplasma is a small bacterium (diameter 0.2 μm) without cell wall, immune to common antibiotic, capable of passing through bacterial filters and of great importance because of its common presence in cell cultures in clinical, research and biopharmaceutical industry . Besides, Mycoplasma is also related with the development of important pathologies such as pneumonia, rheumatoid arthritis, cancer and non‐gonococcal urogenital diseases, among others …”

Section: Methodsmentioning

confidence: 99%

A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina

et al. 2017

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An anoporous anodica lumina( NAA)-based sensors ystem for the detection of Mycoplasma was developed through the implementation of "molecular gates" selectivet ot he presence of this bacterium. The capped support showed an egligible cargo release, while presence of Mycoplasma genomic DNA resulted in the release of rhodamine Bf luorescent dye. This sensor system presentsalimit of detection of 20 genomic DNA co-pies·mL À1 and was applied to the detection of Mycoplasma bacteria in competitive environments, such as culture cell media.Mycoplasma is as mall bacterium (diameter0 .2 mm) without cell wall, immunet oc ommon antibiotic, capable of passing through bacterial filters and of great importance because of its commonp resence in cell cultures in clinical, research and biopharmaceutical industry. [1] Besides, Mycoplasma is also related with the development of important pathologies such as pneumonia,r heumatoid arthritis, cancer andn on-gonococcal urogenital diseases, among others. [2][3][4][5][6] Historically,p olymerase chain reaction( PCR) methods and culture-based approaches have been used extensively for Mycoplasma detection. However,e ven thought hese methods are very sensitive, they have several drawbacks such as high cost, the need of trained personnel, and ar elativelyl ong time before results are obtained. [7] As an alternative, the design of new sensings ystemsf or Mycoplasma detection that overcome the cited drawbacks are of importance and have been recently

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Diagnóstico serológico de las infecciones por Mycoplasma pneumoniae

Cited by 14 publications

References 12 publications

Mycoplasma pneumoniae: A significant but underrated pathogen in paediatric community-acquired lower respiratory tract infections

Mycoplasma pneumoniae: A significant but underrated pathogen in paediatric community-acquired lower respiratory tract infections

Clinical spectrum and diagnostic yields of Mycoplasma pneumoniae as a causative agent of community-acquired pneumonia

A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina

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