A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina

Abstract

An anoporous anodica lumina( NAA)-based sensors ystem for the detection of Mycoplasma was developed through the implementation of "molecular gates" selectivet ot he presence of this bacterium. The capped support showed an egligible cargo release, while presence of Mycoplasma genomic DNA resulted in the release of rhodamine Bf luorescent dye. This sensor system presentsalimit of detection of 20 genomic DNA co-pies·mL À1 and was applied to the detection of Mycoplasma bacteria in competitive environments, such as culture cell media.Mycoplasma is as mall bacterium (diameter0 .2 mm) without cell wall, immunet oc ommon antibiotic, capable of passing through bacterial filters and of great importance because of its commonp resence in cell cultures in clinical, research and biopharmaceutical industry. [1] Besides, Mycoplasma is also related with the development of important pathologies such as pneumonia,r heumatoid arthritis, cancer andn on-gonococcal urogenital diseases, among others. [2][3][4][5][6] Historically,p olymerase chain reaction( PCR) methods and culture-based approaches have been used extensively for Mycoplasma detection. However,e ven thought hese methods are very sensitive, they have several drawbacks such as high cost, the need of trained personnel, and ar elativelyl ong time before results are obtained. [7] As an alternative, the design of new sensings ystemsf or Mycoplasma detection that overcome the cited drawbacks are of importance and have been recently

Recent Advances in Nanoporous Anodic Alumina: Principles, Engineering, and Applications

“…Ethanol is a common electrolyte additive that enables to perform anodization below 0 °C reaching lower current densities. It allows to yield even smaller pores, for example 8 nm diameter pores for the sulfuric-NAA [26,96,97]. It was recently demonstrated that addition of ethanol improves the formation of NAA in sulfuric acid, suppressing its chemical dissolution and the anodization rate.…”

Overview of the Evolution of Silica-Based Chromo-Fluorogenic Nanosensors

“…The results were compared with PCR method, obtaining comparable results in terms of selectivity and sensitivity. Closely related materials but using nanoporous anodic alumina instead of mesoporous silica were recently developed for the detection Candida albicans [39] and Mycoplasma fermentans [40].…”

Nanoporous Anodic Alumina Photonic Crystals for Optical Chemo- and Biosensing: Fundamentals, Advances, and Perspectives

“…SAMs of silanes with hydrophobic terminal groups increase the hydrophobicity of NAA [ 107 ], SAMs of perfluoroalkylchorosilanes reduce the effective pore diameter of NAA for molecular transport [ 108 ], poly(ethylene glycol)silane SAMs improve the biocompatibility of NAA for immunoisolation [ 109 ], and SAMs of mercaptosilanes have been used for sensing applications [ 110 , 111 , 112 , 113 ]. Similar to SAMs of alkanoic and phosphonic acids, SAMs of organosilanes can act as chemical linkers for further immobilization of biomolecules [ 114 , 115 , 116 ], polymers [ 116 ], and nanoparticles [ 117 ]. Furthermore, organosilanes containing different terminal functionalities (e.g., amine, thiol, carboxyl, etc.)…”