Diagnostic Values of miR-221-3p in Serum and Cerebrospinal Fluid for Post-Stroke Depression and Analysis of Risk Factors

Cui, Yan; Ma, Guodi; Fan-ying, Kong; Song, Lijin

doi:10.18502/ijph.v50i6.6423

Cited by 9 publications

(12 citation statements)

References 28 publications

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“…Accordingly, our results revealed that m6A level was decreased as a response to METTL3 downregulation, and miR‐221‐3p in hippocampal tissues showed the same trend of METTL3, while pri‐miR‐221 showed an opposite trend of METTL3. miR‐221‐3p was previously found to be upregulated in the serum and cerebrospinal fluid of DD patients, and increased miR‐221‐3p indicates a high risk of DD (Cui et al, 2021; Enatescu et al, 2016; Feng et al, 2019). To analyze the role of miR‐221‐3p in DD, miR‐221‐3p was overexpressed in CUMS rats and combined with silencing METTL3.…”

Section: Discussionmentioning

confidence: 99%

“…Dysfunctional miRNAs are associated with stress vulnerability, which further leads to an aggravation of DD symptoms (Allen & Dwivedi, 2020; Lopizzo, Zonca, Cattane, Pariante, & Cattaneo, 2019). Of note, miR‐221‐3p is found to be enriched in the serum and cerebrospinal fluid of DD patients and increased miR‐221‐3p indicates high depressive mood scores (Cui, Ma, Kong, & Song, 2021; Feng et al, 2019). In terms of its downstream mechanism, GRB2‐associated binding protein 1 (Gab1) was predicted to be a target of miR‐221‐3p by databases.…”

Section: Introductionmentioning

confidence: 99%

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Mechanism of METTL3‐mediated m6A modification in depression‐induced cognitive deficits

Niu

Wang

Zhou

2022

American J of Med Genetics Pt B

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Depressive disorder (DD) is associated with N6-methyladenosine (m6A) hypermethylation. This study sought to explore the molecular mechanism of Methyltransferase-like 3 (METTL3) in cognitive deficits of chronic unpredictable mild stress (CUMS)-treated rats and provide novel targets for DD treatment. A DD rat model was established via CUMS treatment. Cognitive deficits were assessed via body weighing and behavioral tests. METTL3, microRNA (miR)-221-3p, pri-miR-221, GRB2-associated binding protein 1 (Gab1) expressions in hippocampal tissues were detected via RT-qPCR and Western blotting. m6A, DiGeorge syndrome critical region gene 8 (DGCR8)-bound pri-miR-221 and pri-miR-221 m6A levels were measured. The binding relationship between miR-221-3p and Gab1 was testified by dual-luciferase and RNA pull-down assays. Rescue experiments were designed to confirm the role of miR-221-3p and Gab1. METTL3 was highly expressed in CUMS rats, and silencing METTL3 attenuated cognitive deficits of CUMS rats. METTL3-mediated m6A modification facilitated processing and maturation of pri-miR-221 via DGCR8 to upregulate miR-221-3p. miR-221-3p targeted Gab1. miR-221-3p overexpression or Gab1 downregulation reversed the role of silencing METTL3 in CUMS rats. Overall, METTL3-mediated m6A modification facilitated processing and maturation of pri-miR-221 to upregulate miR-221-3p and then inhibit Gab1, thereby aggravating cognitive deficits of CUMS rats.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mechanism of METTL3‐mediated m6A modification in depression‐induced cognitive deficits

Niu

Wang

Zhou

2022

American J of Med Genetics Pt B

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“…Most importantly, we established ROC curves for plasma microRNA-221-3p levels, and the results showed excellent performance for POCD identification. Previous studies have reported that microRNA-221-3p is related to the pathogenesis of many cerebrovascular diseases, the microRNA-221-3p levels in plasma and CSF can be used as risk and diagnostic indicators of PSD (poststroke depression), moreover, the upregulated plasma microRNA-133b and microRNA-221-3p levels may be biomarkers for early PD [ 26 ]. In addition, microRNA-221-3p plays a role in the autophagy of dopaminergic neurons in a mouse model of PD [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Plasma microRNA-221-3p as a biomarker for POCD after non-cardiac surgery

et al. 2022

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Our previous study showed that the plasma microRNA-221-3p level could serve as a biomarker for major depression or mood. This study aimed to further investigate the role of plasma microRNA-221-3p level in postoperative cognitive dysfunction (POCD). Patients undergoing non-cardiac surgery were randomly assigned according to the inclusion and exclusion criteria. POCD was diagnosed by the Z score method. The relative level of plasma microRNA-221-3p was decided by quantitative real-time polymerase chain reaction. Multiple logistic regression analysis and receiver operating characteristic(ROC) curves were used for the analysis of plasma microRNA-221-3p prediction performance for POCD. At 7 days post-surgery, the rate of POCD was 34.04%. Patients in the POCD group had a higher preoperative depression score, older age, and longer operation duration than that in the NPOCD group. The relative level of plasma microRNA-221-3p in the POCD group was 1.78 and 2.73 times higher than that in the NPOCD group at 1 day before and 7 days after the surgery, respectively. The relative content of plasma microRNA-221-3p at 7 days after operation was an independent risk factor for POCD. The ROC curves showed that the area under the curve was 0.938 for plasma microRNA-221-3p at postoperative 7 days, and the threshold for POCD detection was 12.33 with a sensitivity and specificity of 81.3% and 96.3%, respectively. Our results indicate that the plasma postoperative microRNA-221-3p levels could be an effective predictor for POCD after non-cardiac surgery.

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“…MMSE score was used to define cognitive recovery in one study at 14 days after stroke [58], whereas MoCA score assessed cognitive impairments in three studies in the chronic phase of stroke patients (within 1 year) [50,51,55]. To measure depression symptoms among post-stroke patients, three studies evaluated HAMD scores within 2-3 weeks and at 3 months after stroke onset [99][100][101].…”

Section: Prognostic Tools and Prediction Of Physical Recovery In Is P...mentioning

confidence: 99%

Circulating MicroRNAs and Extracellular Vesicle-Derived MicroRNAs as Predictors of Functional Recovery in Ischemic Stroke Patients: A Systematic Review and Meta-Analysis

Burlacu

Ciobanu

Bădulescu

et al. 2022

IJMS

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Stroke accounts for the second leading cause of death and a major cause of disability, with limited therapeutic strategy in both the acute and chronic phases. Blood-based biomarkers are intensively researched and widely recognized as useful tools to predict the prognoses of patients confronted with therapeutically limited diseases. We performed a systematic review of the circulating biomarkers in IS patients with prognostic value, with a focus on microRNAs and exosomes as predictive biomarkers of motor and cognitive recovery. We identified 63 studies, totalizing 72 circulating biomarkers with prognostic value in stroke recovery, as follows: 68 miRNAs and exosomal-miRNAs being identified as predictive for motor recovery after stroke, and seven biomarkers being predictive for cognitive recovery. Twelve meta-analyses were performed using effect sizes (random-effects and fixed-effects model). The most significant correlation findings obtained after pooling were with miR-21, miR-29b, miR-125b-5p, miR-126, and miR-335. We identified several miRNAs that were correlated with clinical outcomes of stroke severity and recovery after ischemic stroke, providing predictive information on motor and cognitive recovery. Based on the current state of research, we identified serum miR-9 and neutrophil miR-29b as the most promising biomarkers for in-depth follow-up studies, followed by serum miR-124 and plasma miR-125b.

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Diagnostic Values of miR-221-3p in Serum and Cerebrospinal Fluid for Post-Stroke Depression and Analysis of Risk Factors

Cited by 9 publications

References 28 publications

Mechanism of METTL3‐mediated m6A modification in depression‐induced cognitive deficits

Mechanism of METTL3‐mediated m6A modification in depression‐induced cognitive deficits

Plasma microRNA-221-3p as a biomarker for POCD after non-cardiac surgery

Circulating MicroRNAs and Extracellular Vesicle-Derived MicroRNAs as Predictors of Functional Recovery in Ischemic Stroke Patients: A Systematic Review and Meta-Analysis

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