Diagnostic value of the polymerase chain reaction for Chlamydia detection as determined in a follow-up study

Claas, H. C. J.; Wagenvoort, J. H. T.; Niesters, H. G. M.; Tio, T. T.; Rijsoort-Vos, J. H. Van; Quint, Wim

doi:10.1128/jcm.29.1.42-45.1991

Cited by 87 publications

(29 citation statements)

References 16 publications

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“…Vogels and coworkers 15 recommend that practitioners wait for at least 2 weeks after initiation of treatment in humans before using PCR as a test-of-cure because of the presence of positive PCR samples from treated patients when cell culture had become negative. However, Claas et al 20 found good correlation between PCR and culture after 1 week of doxycycline treatment in humans.…”

mentioning

confidence: 99%

Comparison of the Polymerase Chain Reaction and Culture for the Detection of Feline Chlamydia psittaci in Untreated and Doxycycline-Treated Experimentally Infected Cats

Sykes¹,

Studdert²,

Browning³

1999

J Vet Int Med

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The diagnostic sensitivity of the polymerase chain reaction (PCR) was compared with that of culture on conjunctival swabs over the course of infection in 4 doxycycline-treated and 4 untreated cats that were experimentally infected with feline Chlamydia psittaci. Treated cats were given 25 mg (5 mg/kg) of doxycycline orally twice daily for 3 weeks from day 6 after challenge. Clinical signs improved within 3 days of institution of treatment. Culture remained positive for 1 day and PCR remained positive for up to 5 days after treatment was commenced. No recurrence of clinical signs occurred and the organism could not be detected by either PCR or culture for 2 weeks after cessation of therapy. In the 4 untreated cats, conjunctival swabs were taken daily to day 14 and every 2nd weekday to day 64 after challenge. PCR was significantly more sensitive than culture in untreated cats overall (PCR 85.7%, culture 72.9%, P approximately 0) and for cats with clinical signs (PCR 89.2%, culture 79.2%, P = .008). PCR and culture had equivalent sensitivity (100%) for cats showing clinical signs in the 1st month of infection, whereas PCR was considerably more sensitive than culture for cats showing clinical signs in the 2nd month (PCR 72.9%, culture 47.9%, P = .028). Organisms were not detected by PCR in blood or any tissue collected from treated or untreated cats at postmortem. Thus, effective treatment of chlamydiosis in cats is possible with much shorter treatment regimens than currently recommended, and PCR is the more sensitive diagnostic method in chronically infected cats.

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confidence: 99%

Comparison of the Polymerase Chain Reaction and Culture for the Detection of Feline Chlamydia psittaci in Untreated and Doxycycline-Treated Experimentally Infected Cats

Sykes¹,

Studdert²,

Browning³

1999

J Vet Int Med

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“…However, a sensitive, reliable, and inexpensive assay to detect C. trachomatis in specimens that can be easily collected is urgently needed. At present, the most sensitive assay is based on DNA amplification (8,9,15,(18)(19)(20). Recently, C. trachomatis has been shown to be detectable in urine specimens of symptomatic and asymptomatic males by enzyme-linked immunoassays and PCR (6,14,21).…”

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confidence: 99%

Detection of Chlamydia trachomatis infection in urine samples from men and women by ligase chain reaction

et al. 1995

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The suitability of urine specimens from women and men for the detection of Chlamydia trachomatis infection by a ligase chain reaction (LCR)-based assay with plasmid primers was examined with a group of patients attending a sexually transmitted disease clinic in Amsterdam, The Netherlands. Cervical specimens from 15 of 237 (6.3%) women tested positive for C. trachomatis by cell culture. Of the 25 (10.5%) female urine samples that tested positive by the plasmid-LCR assay, 13 were obtained from cervical culture-positive women. Nine of the 12 plasmid-LCR-positive urine samples from cervical culture-negative women were confirmed to be positive by a second LCR assay with primers based on chromosomal DNA. Urethral specimens from 24 of 258 (9.3%) men were positive for C. trachomatis infection by cell culture. Of the 25 (9.7%) urine samples that tested positive by plasmid-LCR, 20 were from culture-positive men. All five of the LCR-positive urine samples from culturenegative men were confirmed to be positive by the LCR with chromosomal DNA primers. Relative to cell culture, testing by plasmid-LCR analysis of male urine samples had a sensitivity of 83.3% and a specificity of 97.9%; after resolution of discordant samples, these values were 86.2 and 100%, respectively. In the study with women, the sensitivities of plasmid-LCR analysis of cervical and urine specimens in comparison with cervical cell culture were 93.3 and 86.7%, respectively. After resolution of discrepant samples, the sensitivities of the plasmid-LCR test for cervical swabs and female urine samples were 96.3 and 92.6%, respectively. These results indicate that the plasmid-LCR-based assay is a very reliable, sensitive, convenient test for the detection of C. trachomatis infection in female and male urine specimens.

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“…Sample volumes of 1 ml or more can easily be concentrated to 100 l, and of this concentrated DNA, a predetermined volume is added to the PCR mixture. Claas et al (2,3) applied 100 l of an initial sample volume to a final PCR analysis. By making use of this procedure, they established a PCR sensitivity of 100% and a specificity of 98.5% compared with Chlamydia cell culture (CC).…”

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confidence: 99%

Influence of volume of sample processed on detection of Chlamydia trachomatis in urogenital samples by PCR

et al. 1995

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In the present study, it was demonstrated that the sensitivity of the PCR for the detection of Chlamydia trachomatis is influenced by the volume of the clinical sample which is processed in the PCR. An adequate sensitivity for PCR was established by processing at least 4%, i.e., 80 l, of the clinical sample volume per PCR. By using this preparation procedure, 1,110 clinical samples were evaluated by PCR and by cell culture, and results were compared. After discordant analysis, cell culture resulted in a sensitivity of 79.1% and PCR resulted in a sensitivity of 92.7%. Furthermore, it was shown that treatment with antibiotics immediately resulted in negative cell culture results but that PCR could give positive results up to 2 weeks posttreatment.

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Diagnostic value of the polymerase chain reaction for Chlamydia detection as determined in a follow-up study

Cited by 87 publications

References 16 publications

Comparison of the Polymerase Chain Reaction and Culture for the Detection of Feline Chlamydia psittaci in Untreated and Doxycycline-Treated Experimentally Infected Cats

Comparison of the Polymerase Chain Reaction and Culture for the Detection of Feline Chlamydia psittaci in Untreated and Doxycycline-Treated Experimentally Infected Cats

Detection of Chlamydia trachomatis infection in urine samples from men and women by ligase chain reaction

Influence of volume of sample processed on detection of Chlamydia trachomatis in urogenital samples by PCR

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