Influence of volume of sample processed on detection of Chlamydia trachomatis in urogenital samples by PCR

Goessens, W. H. F.; Kluytmans, Jan; Toom, N. Den; Rijsoort-Vos, T. H. Van; Niesters, Bert; Stolz, E.; Verbrugh, Henri A.; Quint, Wim

doi:10.1128/jcm.33.1.251-253.1995

Cited by 21 publications

(9 citation statements)

References 14 publications

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“…No patient was LCR positive but Roche PCR negative. The lower sensitivity of the MOMP PCR is consistent with previous reports of the use of the MOMP PCR to test genital specimens (7,12,13) and with the in vitro sensitivity data described above.…”

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confidence: 90%

Detection of Chlamydia trachomatis in urine samples by nucleic acid tests: comparison with culture and enzyme immunoassay of genital swab specimens

et al. 1997

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Two commercially available nucleic acid-based tests, ligase chain reaction (LCR; Abbott Laboratories) and PCR (Roche Diagnostics), for the detection of Chlamydia trachomatis in male and female urine samples were compared with culture and enzyme immunoassay (EIA) (Microtrak; Syva) for C. trachomatis detection in genital samples. The samples were collected from 1,005 patients who attended a sexually transmitted disease clinic. In this study population, the prevalence of the infection was 4%. Specimens which were reactive in any of the tests were retested with a different PCR test using primers directed against the major outer membrane protein gene. With a "gold standard" of a positive culture, or any other positive test result if it was confirmed by an independent test, the Roche PCR (95% sensitive, 99.9% specific) was more sensitive than the LCR (75% sensitive, 100% specific) ( 2 , P < 0.0001) while both tests were more sensitive than culture (58% sensitive, 100% specific) or EIA (45% sensitive, 100% specific) ( 2 , P < 0.001). The Roche PCR and Abbott LCR tests of urine identified 65% and 30% more positive patients, respectively, than did testing by culture of urethral or cervical specimens. Nucleic acid testing of urine specimens for C. trachomatis is a more sensitive and convenient method for the detection of genital infection.

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confidence: 90%

Detection of Chlamydia trachomatis in urine samples by nucleic acid tests: comparison with culture and enzyme immunoassay of genital swab specimens

et al. 1997

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“…This is best demonstrated by evaluating our own data. In a previous study (13) at our institute, a prevalence of 7.8% by tissue culture for C. trachomatis infection was reported. In that study an in-house PCR on US and CS specimens was evaluated.…”

Section: Discussionmentioning

confidence: 78%

“…(ii) Cervical and urethral swabs. Specimens for PCR analysis were treated as described before (13). Briefly, a 400-l sample was centrifuged for 30 min at 14,000 ϫ g, and the pellet was treated with 40 l of lysis buffer.…”

Section: Methodsmentioning

confidence: 99%

Comparison of three commercially available amplification assays, AMP CT, LCx, and COBAS AMPLICOR, for detection of Chlamydia trachomatis in first-void urine

Goessens¹,

Mouton²,

Meijden³

et al. 1997

J Clin Microbiol

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We compared the Gen-Probe transcription-mediated amplification assay (AMP CT), the Abbott LCx assay, and the Roche COBAS AMPLICOR assay for the detection of Chlamydia trachomatis in a mixed population in urine samples. First-void urine, urethral specimens, and cervical specimens in females were obtained from 1,000 patients (544 males and 456 females) visiting the outpatient sexually transmitted disease clinic of our hospital. The prevalence of C. trachomatis infection was 7.7% as determined by tissue culture of urethral and cervical specimens. The sensitivities of LCx, COBAS AMPLICOR, and AMP CT compared to cell culture were 79, 86, and 78%, respectively. Sensitivity and specificity were recalculated by using a new "gold standard", i.e., a sample was considered to be true positive if two or more techniques yielded positive results. Specimens positive only by cell culture or positive in only one commercial amplification technique were retested by a previously described in-house PCR. After discordance analysis the sensitivities of LCx, COBAS AMPLICOR, and AMP CT were 84, 93, and 85%, respectively. Specificity exceeded 99% for all three assays. With each method the sensitivity was lower for urine samples from females compared to urine samples from males. By application of this new gold standard, existing differences between methods are highlighted; future evaluations of new techniques should be validated against two or more amplification assays.

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“…The results of the AMP-CT test were compared with the results of cell culture. An in-house PCR technique was used for analysis of samples with discordant results (12).…”

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confidence: 99%

Detection of Chlamydia trachomatis in male and female urine specimens by using the amplified Chlamydia trachomatis test

et al. 1997

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The amplified Chlamydia trachomatis test (AMP-CT; Gen-Probe), a new diagnostic test for the detection of Chlamydia trachomatis, was evaluated with urine specimens from 1,000 patients visiting the outpatient department for sexually transmitted diseases at the University Hospital Rotterdam, Rotterdam, The Netherlands, by comparing the results to those of cell culture. From February 1996 to July 1996, urine samples for the AMP-CT test and urethral swabs for cell culture were collected from 544 men, while cervical swabs from 456 women were also taken for cell culture. Positive test results were obtained for 130 (13%) of the patients. AMP-CT test and cell culture results were discordant for 70 (7%) specimens. Analysis of the samples with discordant results was performed by an in-house PCR. After resolution of the discordant results, the sensitivity, specificity, and positive and negative predictive values of the AMP -CT test were 84.3, 98.8, 89.6, and 98%, respectively, for samples from females and 100, 99.2, 93.1, and 100%, respectively, for samples from males, while for cell culture these values were 72.5, 99.2, 92.5, and 98%, respectively, for samples from females and 57.4, 99.0, 86.1, and 95.4%, respectively, for samples from males. We conclude that the AMP-CT test is a fast and reliable test for the detection of C. trachomatis in urine specimens from females and, in particular, males.

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Influence of volume of sample processed on detection of Chlamydia trachomatis in urogenital samples by PCR

Cited by 21 publications

References 14 publications

Detection of Chlamydia trachomatis in urine samples by nucleic acid tests: comparison with culture and enzyme immunoassay of genital swab specimens

Detection of Chlamydia trachomatis in urine samples by nucleic acid tests: comparison with culture and enzyme immunoassay of genital swab specimens

Comparison of three commercially available amplification assays, AMP CT, LCx, and COBAS AMPLICOR, for detection of Chlamydia trachomatis in first-void urine

Detection of Chlamydia trachomatis in male and female urine specimens by using the amplified Chlamydia trachomatis test

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