Diagnostic Value of Serum Adenosine Deaminase Activity in HIV Infected Patients of Kurdish Population

Abdi, Mohammad; Ahmadi, Abbas; Roshany, Daem; Khodadadi, Iraj; Javid, Saman; Shahmohammad-Nezhad, Shiva; Sharifipour, Mozhdeh

doi:10.7754/clin.lab.2012.120631

Cited by 7 publications

(11 citation statements)

References 14 publications

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“…As in our recent work, we found a high sensitivity and specificity for both tADA (88% and 96%, respectively) and ADA2 (93% and 90%, respectively) activities confirming applicability of serum adenosine deaminase as a novel marker in distinguishing HIV positive patients (all mono-and co-infected) from healthy subjects (20). After redefining of cut-off values for CD4 + cell count, tADA, ADA1, and ADA2 enzyme activity, ROC curve analysis for HIV monoinfection against HIV coinfections showed a significant sensitivity, specificity, and area under curve for all tADA, ADA1, and ADA2 enzyme activities with the highest specificity (82%) being observed for tADA and the greatest sensitivity (80%) for ADA1.…”

Section: Table 1 Personal Characteristics Activities Of Liver Enzymsupporting

confidence: 89%

Serum Adenosine Deaminase (ADA) Activity: A Novel Screening Test to Differentiate HIV Monoinfection From HIV‐HBV and HIV‐HCV Coinfections

Abdi

Rahbari

Khatooni

et al. 2015

Clinical Laboratory Analysis

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Section: Table 1 Personal Characteristics Activities Of Liver Enzymsupporting

confidence: 89%

Serum Adenosine Deaminase (ADA) Activity: A Novel Screening Test to Differentiate HIV Monoinfection From HIV‐HBV and HIV‐HCV Coinfections

Abdi

Rahbari

Khatooni

et al. 2015

Clinical Laboratory Analysis

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“…However, further studies are required to simultaneously investigate ADA activity and the concentration of adenosine in serum or follicular fluid and to confirm the role of lower adenosine deaminase activity in the onset or in the development of PCOS. Apart from the important role of ADA activity in diseases such as severe combined immunodeficiency [Bose and Nandagopal 2013], pulmonary tuberculosis [Salmanzadeh et al 2015], HIV [Abdi et al 2013], breast cancer [Mahajan et al 2013], multiple sclerosis [Polachini et al 2014], arthritis [Doudkani-Fard et al 2014], and infertility [Fattahi et al 2015], ADA polymorphic genotype variations have also been found as key players in the development of many pathological conditions. Among over 1,450 known single nucleotide ADA1 gene polymorphisms, the G22A polymorphism has attracted much more attention.…”

Section: Discussionmentioning

confidence: 99%

Association of G22A and A4223C ADA1 gene polymorphisms and ADA activity with PCOS

Salehabadi

Farimani²,

Tavilani

et al. 2016

Systems Biology in Reproductive Medicine

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Adenosine deaminase-1 (ADA1) regulates the concentration of adenosine as the main modulator of oocyte maturation. There is compelling evidence for the association of ADA1 gene polymorphisms with many diseases but the importance of ADA1 polymorphisms in polycystic ovary syndrome (PCOS) has not been studied before. This study investigates serum total ADA activity (tADA), ADA1 and ADA2 isoenzyme activities, and genotype and allele frequencies of G22A and A4223C polymorphisms in healthy and PCOS women. In this case-control study 200 PCOS patients and 200 healthy women were enrolled. Genomic DNA was extracted from whole blood and the PCR-RFLP technique was used to determine the G22A and A4223C variants. The genotype frequencies were calculated and the association between polymorphic genotypes and enzyme activities were determined. tADA activity was significantly lower in the PCOS group compared with the control group (27.76±6.0 vs. 39.63±7.48, respectively). PCOS patients also showed reduced activity of ADA1 and ADA2. PCOS was not associated with G22A polymorphism whereas AA, AC, and CC genotypes of A4223C polymorphism were found distributed differently between the control and the PCOS women where the C allele showed a strong protective role for PCOS (odds ratio=1.876, p=0.033). The present study for the first time showed that lower ADA activity may be involved in pathogenesis of PCOS by maintaining a higher concentration of adenosine affecting follicular growth. As a novel finding, we also showed great differences in genotype distribution and allele frequencies of A4223C polymorphism between groups indicating a protective role for C allele against PCOS. AbbreviationsADA: adenosine deaminase PCOS: polycystic ovary syndrome PCR-RFLP: polymerase chain reaction-restriction fragment length polymorphism tADA: total adenosine deaminase.

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“…Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio, and negative likelihood ratio were also calculated. [ 21 22 ]…”

Section: Methodsmentioning

confidence: 99%

Diagnostic value of cystatin C for diagnosis of early renal damages in type 2 diabetic mellitus patients: The first experience in Iran

et al. 2015

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Background:Diabetic nephropathy (DN) is one of the most important complications of diabetes mellitus. Now-a-days, cystatin C (CysC) is introduced as a new marker for diagnosis of renal damages; however, use of this marker in clinical laboratories is still controversial. The present study was aimed to evaluate the diagnostic value of serum CysC for early detection or monitoring treatment of kidney damages in the Kurdish people with type 2 diabetes mellitus.Materials and Methods:Glomerular filtration rate (GFR) was estimated by Modification of Diet in Renal Disease formula. Serum CysC and urine microalbumin were also measured in 126 diabetic and healthy subjects. Blood glycated hemoglobin (Hb) also measured in all healthy and diabetic patients. Two independent samples t-test, Mann-Whitney U-test, one-way ANOVA, and Kruskal-Wallis test, as well as Pearson/Spearman correlation coefficient statistical tests were used as appropriate.Results:Serum CysC was higher (1312.41 ng/ml) in diabetic patients with GFR <60 ml/min than other subjects (993.25 ng/ml) (patients with normal kidney function and healthy subjects). A borderline significant correlation between CysC and estimating GFR (rs = −0.16, P = 0.05) but highly significant with microalbumin (rs = 0.22, P = 0.014) was observed. Serum CysC sensitivity, negative and positive predictive values were 100 and 4%.Conclusion:CysC cover variation of GFR and urine microalbumin, but it cannot be used as a surrogating marker of glycated Hb. According to our results, it seems that serum CysC is a useful marker for screening of DN; but it cannot be used for monitoring of treatment in diabetic patients.

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Diagnostic Value of Serum Adenosine Deaminase Activity in HIV Infected Patients of Kurdish Population

Cited by 7 publications

References 14 publications

Serum Adenosine Deaminase (ADA) Activity: A Novel Screening Test to Differentiate HIV Monoinfection From HIV‐HBV and HIV‐HCV Coinfections

Serum Adenosine Deaminase (ADA) Activity: A Novel Screening Test to Differentiate HIV Monoinfection From HIV‐HBV and HIV‐HCV Coinfections

Association of G22A and A4223C ADA1 gene polymorphisms and ADA activity with PCOS

Diagnostic value of cystatin C for diagnosis of early renal damages in type 2 diabetic mellitus patients: The first experience in Iran

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