Serum Adenosine Deaminase (ADA) Activity: A Novel Screening Test to Differentiate HIV Monoinfection From HIV‐HBV and HIV‐HCV Coinfections

Abdi, Mohammad; Rahbari, Rizgar; Khatooni, Zahed; Naseri, Nima; Najafi, Arvin; Khodadadi, Iraj

doi:10.1002/jcla.21836

Cited by 11 publications

(17 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the study of Chilo L. et al, CD4 counts of patients with HIV/HBV infection were significantly lower than mono-infected patients (30). Another study also demonstrated low amount of CD4 cells in co-infected patients (31). In the present study, the mean number of CD4 cells in patients was 120 (cells/μL).…”

Section: Discussionsupporting

confidence: 55%

Mutations in the Basal Core Promoter and Precore/Core Regions of Hepatitis B Virus in Patients Co-Infected With Human Immunodeficiency Virus

et al. 2016

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 55%

Mutations in the Basal Core Promoter and Precore/Core Regions of Hepatitis B Virus in Patients Co-Infected With Human Immunodeficiency Virus

et al. 2016

View full text Add to dashboard Cite

“…Deficiency of ADA activity is associated with defects related to immunological development, which can result in hematologic malignancies (Li, Nakagome, Hirono, Itoh, & Fujiwara, 2015). However, high levels of ADA in serum are associated with hepatic diseases (Abdi et al, 2016;Torgutalp, Efe, Babaoglu, & Kav, 2017). Serum ADA activity is also significantly increased in patients with chronic hepatitis, cirrhosis, and hepatocellular carcinoma, and thus this factor can be used as a screening index for chronic liver disease.…”

Section: Meijermentioning

confidence: 99%

A rapid assay to screen adenosine deaminase inhibitors from Ligustri Lucidi Fructus against metabolism of cordycepin utilizing ultra‐high‐performance liquid chromatography–tandem mass spectrometry

Guan

Liu

et al. 2020

Biomedical Chromatography

View full text Add to dashboard Cite

Cordycepin has recently received increased attention owing to its extensive pharmacological activity. Adenosine deaminase (ADA) is widely distributed in mammalian blood and tissues; as a result, cordycepin is quickly metabolized upon entering into the body and converted into the inactive metabolite 3′‐deoxyinosine, thus limiting its activity when administered alone. We herein present a novel ultra‐high‐performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method for screening ADA inhibitors against the metabolism of cordycepin. Cordycepin and 3′‐deoxyinosine were chosen as substrate and product, respectively. A proper separation was achieved for all analytes within 3 min. 3′‐Deoxyinosine was quantified in the presence or absence of potential ADA inhibitors to evaluate ADA activity. The assay can simultaneously determine substrate and product, with the endogenous substance and ADA inhibitors added not interfering in its activity. After optimizing the enzymatic incubation and UHPLC–MS/MS conditions, Km and Vmax values for ADA deamination of cordycepin were 95.18 ± 7.85 μm and 363.90 ± 12.16 μmol/min/unit, respectively. Oleanolic acid and ursolic acid from Ligustri Lucidi Fructus were chosen as ADA inhibitors with half maximal inhibitory concentration values of 21.82 ± 0.39 and 18.41 ± 0.14 μm, respectively. A non‐competitive inhibition model was constructed and this assay can be used to screen other potential ADA inhibitors quickly and accurately.

show abstract

“…HIV infection disturbs CD26/ADA costimulation, highjacks the immunomodulatory actions of Treg cells, and stimulates the generation of adenosine [108], in addition to downregulate ADA expression in lymphocytes [86]. In contrast, ADA enzymatic activity is reportedly increased in serum [103,104,[115][116][117][118][119][120] and plasma of HIV patients [121][122][123] to overcome the effects of excessive adenosine levels.…”

Section: Ada In the Context Of Hiv Pathogenesismentioning

confidence: 99%

“…ADA activity in HIV patients has long been evaluated in serum and plasma [121][122][123] lymphocytes [124], erythrocytes [125,126], and platelets [71]. Correlations between ADA activity and several clinical parameters such as seroconversion [121,126], prognosis [84, 97-100, 104, 107], immune activation [118], CD4 + T cell count [115,[118][119][120]123], viral load [118], CD4/CD8 ratio [119], coinfection [115,120], and treatment outcome [71,117,119,123] are discussed. Most of these studies aimed to use ADA activity as a biomarker but also helped to understand the pathological processes involved in the infection.…”

Section: Ada In the Context Of Hiv Pathogenesismentioning

confidence: 99%

See 1 more Smart Citation

Adenosine signaling and adenosine deaminase regulation of immune responses: impact on the immunopathogenesis of HIV infection

Passos

Bernardes

Silva

et al. 2018

Purinergic Signalling

View full text Add to dashboard Cite

Infection by human immunodeficiency virus (HIV) causes the acquired immune deficiency syndrome (AIDS), which has devastating effects on the host immune system. HIV entry into host cells and subsequent viral replication induce a proinflammatory response, hyperactivating immune cells and leading them to death, disfunction, and exhaustion. Adenosine is an immunomodulatory molecule that suppresses immune cell function to protect tissue integrity. The anti-inflammatory properties of adenosine modulate the chronic inflammation and immune activation caused by HIV. Lack of adenosine contributes to pathogenic events in HIV infection. However, immunosuppression by adenosine has its shortcomings, such as impairing the immune response, hindering the elimination of the virus and control of viral replication. By attempting to control inflammation, adenosine feeds a pathogenic cycle affecting immune cells. Deamination of adenosine by ADA (adenosine deaminase) counteracts the negative effects of adenosine in immune cells, boosting the immune response. This review comprises the connection between adenosinergic system and HIV immunopathogenesis, exploring defects in immune cell function and the role of ADA in protecting these cells against damage.

show abstract

Serum Adenosine Deaminase (ADA) Activity: A Novel Screening Test to Differentiate HIV Monoinfection From HIV‐HBV and HIV‐HCV Coinfections

Cited by 11 publications

References 18 publications

Mutations in the Basal Core Promoter and Precore/Core Regions of Hepatitis B Virus in Patients Co-Infected With Human Immunodeficiency Virus

Mutations in the Basal Core Promoter and Precore/Core Regions of Hepatitis B Virus in Patients Co-Infected With Human Immunodeficiency Virus

A rapid assay to screen adenosine deaminase inhibitors from Ligustri Lucidi Fructus against metabolism of cordycepin utilizing ultra‐high‐performance liquid chromatography–tandem mass spectrometry

Adenosine signaling and adenosine deaminase regulation of immune responses: impact on the immunopathogenesis of HIV infection

Contact Info

Product

Resources

About