Diagnostic value of rapid urease test and urea breath test for Helicobacter pylori detection in patients with Billroth II gastrectomy: A prospective controlled trial

Adamopoulos, Adam; Stergiou, George S.; Sakizlis, G.N.; Tiniakos, D.; Nasothimiou, Efthimia; Sioutis, D.K.; Achimastos, Apostolos

doi:10.1016/j.dld.2008.05.010

Cited by 25 publications

(11 citation statements)

References 16 publications

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“…Infection of H. pylori should also be eradicated in the gastric remnant of the patient who underwent partial to subtotal gastrectomy for gastric cancer. UBT is not reliable for the determination of H. pylori infection in the gastric remnant after distal gastrectomy [13], but stool antigen tests have been shown to be useful in this case [14]. Furthermore, stool antigen tests are cost-effective.…”

Section: Discussionmentioning

confidence: 98%

“…Breath samples were taken before and 20 min after the ingestion of 100 mg 13 C-urea tablet. 13 CO 2 in expired breath was measured using UBit-IR300 (Otsuka Electronics, Osaka, Japan). Results of UBT were considered positive if the d over baseline (DOB) value was greater than 2.5% [8].…”

Section: Methodsmentioning

confidence: 99%

“…For these patients, treatment is cheaper if the results of eradication therapy are confirmed by tests not involving endoscopy. 13 C-urea breath test (UBT) has been considered to be the most reliable non-invasive test for assessing the outcome of eradication therapy and is recommended by the new Japanese guideline [1]. UBT is the most commonly used test for the examination of the results of eradication therapy.…”

Section: Introductionmentioning

confidence: 99%

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Applicability of a rapid stool antigen test, using monoclonal antibody to catalase, for the management of Helicobacter pylori infection

et al. 2011

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Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Applicability of a rapid stool antigen test, using monoclonal antibody to catalase, for the management of Helicobacter pylori infection

et al. 2011

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“…Although many detection methods have been developed for H pylori , most of them require sophisticated instruments and professional operators, which significantly limit early detection and can cause fatal consequences and economic loss. For example, rapid urease test (RUT) and urea breath test (UBT) are common detection methods for H pylori frequently used in clinical practice and histology . Unfortunately, both require copious amounts of time and strict operating environment, and they require actual sample collection from the patients.…”

Section: Discussionmentioning

confidence: 99%

“…For example, rapid urease test (RUT) and urea breath test (UBT) are common detection methods for H pylori frequently used in clinical practice and histology. 43 Unfortunately, both require copious amounts of time and strict operating environment, and they require actual sample collection from the patients. Moreover, studies suggest that H pylori residing in the human stomach can release volatile organic compounds (VOCs) that can be detected in the expired air.…”

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confidence: 99%

Recognition of Helicobacter pylori by protein‐targeting aptamers

Yan

Ren

et al. 2019

Helicobacter

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Background Helicobacter pylori (H pylori) is a disease‐causing pathogen capable of surviving under acidic conditions of the human stomach. Almost half of the world's population is infected with H pylori, with gastric cancer being the most unsatisfactory prognosis. Although H pylori has been discovered 30 years ago, the effective treatment and elimination of H pylori continue to be problematic. Materials and methods In our study, we screened nucleic acid aptamers using H pylori surface recombinant antigens as targets. Trypsin was used for separating aptamers that were bound to proteins. Following nine rounds of screening, we performed sequence similarity analyses to assess whether the aptamers can recognize the target protein. Two sequences with desirable recognition ability were selected for affinity detection. Aptamer Hp4 with the strongest binding ability to the H pylori surface recombinant antigen was chosen. After optimization of the binding conditions, we conducted specificity tests for Hp4 using Escherichia coli, Staphylococcus aureus, Vibrioanguillarum, and H pylori. Results The data indicated that the aptamer Hp4 had an equilibrium dissociation constant (Kd) of 26.48 ± 5.72 nmol/L to the target protein. This aptamer was capable of exclusively detecting H pylori cells, without displaying any specificity for other bacteria. Conclusions We obtained a high‐affinity aptamer for H pylori, which is expected to serve as a new molecular probe for detection of H pylori.

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Non-invasive diagnostic tests forHelicobacter pyloriinfection

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Takwoingi

Siddique

et al. 2018

Cochrane Database of Systematic Reviews

154

130

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In people without a history of gastrectomy and those who have not recently had antibiotics or proton ,pump inhibitors, urea breath tests had high diagnostic accuracy while serology and stool antigen tests were less accurate for diagnosis of Helicobacter pylori infection.This is based on an indirect test comparison (with potential for bias due to confounding), as evidence from direct comparisons was limited or unavailable. The thresholds used for these tests were highly variable and we were unable to identify specific thresholds that might be useful in clinical practice.We need further comparative studies of high methodological quality to obtain more reliable evidence of relative accuracy between the tests. Such studies should be conducted prospectively in a representative spectrum of participants and clearly reported to ensure low risk of bias. Most importantly, studies should prespecify and clearly report thresholds used, and should avoid inappropriate exclusions.

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Diagnostic value of rapid urease test and urea breath test for Helicobacter pylori detection in patients with Billroth II gastrectomy: A prospective controlled trial

Cited by 25 publications

References 16 publications

Applicability of a rapid stool antigen test, using monoclonal antibody to catalase, for the management of Helicobacter pylori infection

Applicability of a rapid stool antigen test, using monoclonal antibody to catalase, for the management of Helicobacter pylori infection

Recognition of Helicobacter pylori by protein‐targeting aptamers

Non-invasive diagnostic tests forHelicobacter pyloriinfection

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