Diagnostic Value of<i>Brucella</i>ELISA IgG and IgM in Bacteremic and Non-Bacteremic Patients with Brucellosis

Osoba, Abimbola O.; Balkhy, Hanan H.; Memish, Ziad A.; Khan, Mohammed Y.; Al-Thagafi, A; Shareef, B Al; Mowallad, A Al; Oni, Gbolahan A.

doi:10.1080/1120009x.2001.11782330

Cited by 37 publications

(36 citation statements)

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“…However, very few comparative publications are available on the evaluation of ELISA in bacteriologically proven cases and the standard serological tests for brucellosis. 11,12 Therefore, the focal points of this work were to evaluate the diagnostic accuracy of ELISA with culture and classical serological methods [SAT and 2-mercaptoethanol (ME) test], which are currently accepted tests for microbiological diagnosis of brucellosis, as well as to determine the utility of ELISA in an area where brucellosis is endemic.…”

Section: Introductionmentioning

confidence: 99%

ELISA versus Conventional Methods of Diagnosing Endemic Brucellosis

Mantur

Parande²,

Amarnath³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Abstract. The diagnostic value of enzyme-linked immunosorbent assay (ELISA) was evaluated when blood specimens of 92 patients suspected of brucellosis underwent the ELISA (IgM and IgG), standard tube agglutination (SAT) , and 2-mercaptoethanol (2-ME) tests and blood cultures; 38 sera from non-brucellosis patients and 34 sera from blood donors were also subjected to ELISA, SAT, and 2 -ME tests. SAT was able to pinpoint only 23 (25%), whereas ELISA confirmed the etiology in 56 (60.9%; P < 0.001) patients with brucellosis, including 31 culture-confirmed cases. The sensitivity and specificity of ELISA were 100% and 71.31%, respectively. Because they were confirmed by ELISA, the diagnosis could never be excluded with SAT in 33 cases. ELISA has been found to be more sensitive in acute (28% higher sensitivity; P < 0.02) and chronic (55% higher sensitivity; P < 0.01) cases. For accurate diagnosis in suspected brucellosis cases detection, we recommend both ELISA IgM and IgG tests. ELISA IgG and 2 -ME tests seem to be promising tools in judging prognosis.

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Section: Introductionmentioning

confidence: 99%

ELISA versus Conventional Methods of Diagnosing Endemic Brucellosis

Mantur

Parande²,

Amarnath³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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“…This finding was also supported by Osoba et al, who confirmed from their study that ELISA IgG was an effective method in diagnosing Brucella infection. [37] From this study, a sensitivity of 66.7%, specificity of 55. [18,38] Queipo-Ortuno and others also made use of the same primers, B4/B5 primer, amplifying a 223-bp fragment of the bcsp31 gene and found 100% sensitivity and 98.3% specificity as compared with 70% constituents of blood culture.…”

Section: Discussionmentioning

confidence: 99%

Missed opportunities for the diagnosis of Brucella infection among slaughterhouse workers at the Kumasi Abattoir, Ghana

Amegashie

Annan

Awuah

et al. 2017

JER

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Brucellosis is a zoonotic disease in humans with its public health importance. Laboratory diagnostic methods targeting brucellosis are not performed in hospital settings across Ghana. Very little is known about the comparative diagnostic abilities of the various tests available presently. The aim of this study therefore was to evaluate and compare diagnostic performances of Rose Bengal Plate Test (RBPT), Enzyme Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction (PCR) employed in diagnosing Brucella infection. Two hundred and twenty Abattoir workers were randomly selected in Kumasi, Ghana. Blood samples were collected, serum extracted and tested for the presence of anti-Brucella antibodies and compared among three different techniques, using ELISA, RBPT and PCR. From the 220 participants tested for antibodies against Brucella spp., 3 (1.4%), 4 (1.8%) and 21 (9.6%) were positive for Rose Bengal Plate test, anti-Brucella ELISA IgM and anti-Brucella ELISA IgG respectively. A total of 98 (44.5%) participants tested positive by PCR. The sensitivity, specificity, positive predictive value, negative predictive values and Kappa value for Rose Bengal in comparison with PCR were 66.7%, 55.8%, 2.0%, 100% and 0.013 respectively while that for ELISA IgG in comparison with PCR were 85.7%, 71.3%, 18.4%, 98.5% and 0.212 respectively. PCR yielded the highest sensitivity and specificity among the three diagnostic methods in this study and should be considered for use at strategic reference laboratories to augment existing routine serological tests for brucella performed in laboratories in Ghana.

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“…Elisa test is a relatively new one diagnostic test has been studied in several studies. Its specificity and sensitivity has been reported as high as 100% (6)(7)(8)10 with Wright test. This shows that using Elisa test has decreased false negative results of diagnostic tests.…”

Section: Discussionmentioning

confidence: 99%

“…For this reason, we decided to compare the diagnostic value of these traditional serological tests with more modern ones like Elisa. Its' sensitivity for Brucella has been reported as 96 to98.2% in different studies, and its specificity as 98.8 to 100% (8,10). Meanwhile several studies recommend simultaneous use of these traditional and modern serological tests (11).…”

Section: Introductionmentioning

confidence: 99%

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Study of the Assimilation Rate of Immunoenzymatic Tests and Traditional Serological Methods in the Diagnosis of Human Brucellosis

Bashiri

Sayad

Madani

2013

Jundishapur J Microbiol

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Background: Brucellosis is a disease carried by animals that can be transmitted to humans. The signs and symptoms of brucellosis are nonspecific, blood tests and blood/tissue cultures are necessary for making the diagnosis of brucellosis. Testing for antibodies against the bacteria and isolating the organism from blood cultures and biopsy of body tissue (from the bone marrow or the liver) are various methods of diagnosis of brucellosis. In the absence of bacteriologic confirmation, a presumptive diagnosis can be made on the basis of high or rising titers of specific antibodies. Objectives: It is observed practically, that the sensitivity of serological tests is less than the amounts mentioned in the reference books. Elisa is a new method for diagnosis of the disease and in this study; application and its assimilation rate are compared with traditional serological tests. Materials and Methods:In this study, patients were selected who had suspicious clinical symptoms of brucellosis. Serological and Elisa tests were performed simultaneously. Results: In this setting, detection rate of the disease is 34.86%, if the high normal titer for the Wrights test titer assumed 1/80 and is 44.95% if the titer assumed 1/40, while Elisa test had detection rate of 58.72% for those suspicious cases. Furthermore, the detection rate for diagnosis of active brucellosis with 2ME tests was 17.54% with high normal titer of 1/80 and was 59.64% with a high normal titer of 1/40. Conclusions: The use of the Elisa tests is preferable for the diagnosis, and if the serological tests are used it is better that the high normal titer assumed to be 1/40.

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Diagnostic Value ofBrucellaELISA IgG and IgM in Bacteremic and Non-Bacteremic Patients with Brucellosis

Cited by 37 publications

References 1 publication

ELISA versus Conventional Methods of Diagnosing Endemic Brucellosis

ELISA versus Conventional Methods of Diagnosing Endemic Brucellosis

Missed opportunities for the diagnosis of Brucella infection among slaughterhouse workers at the Kumasi Abattoir, Ghana

Study of the Assimilation Rate of Immunoenzymatic Tests and Traditional Serological Methods in the Diagnosis of Human Brucellosis

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