Diagnostic test accuracy of the cobas 6800 system for detection of hepatitis C virus viraemia levels from dried blood spots

Gómez, Lorena Vázquez; Reygosa, Cristina; Morales-Arráez, Dalia; Ramos, Ruth; Pérez, Antonia; Hernández, Alberto; Quintero, Enrique; Gutiérrez, Fernando; Díaz‐Flores, Felicitas; Hernández‐Guerra, Manuel

doi:10.1016/j.eimce.2020.04.001

Cited by 5 publications

(16 citation statements)

References 27 publications

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“…The 20 studies investigating the diagnostic accuracy of HCV‐RNA tests in DBS were published between 2002 and 2020 63,67,78,81,82,85,88–101 . Of these, 9 were cross‐sectional, 81,89,90,93–97,100 and 11 were case–control 63,67,78,82,85,88,91,92,98,99,101 .…”

Section: Resultsmentioning

confidence: 99%

“…The 20 studies investigating the diagnostic accuracy of HCV-RNA tests in DBS were published between 2002 and 2020. 63,67,78,81,82,85,[88][89][90][91][92][93][94][95][96][97][98][99][100][101] Of these, 9 were cross-sectional, 81,89,90,93-97,100 and 11 were casecontrol. 63,67,78,82,85,88,91,92,98,99,101 Sixteen studies were from high-income countries, 67,78,81,82,85,[88][89][90][91][92][93][96][97][98][99]101 and 4 were from lower-middle-income countries.…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Overall, 6 of the studies were rated as at low risk of bias, 89,90,94,96,97,100 10 were at moderate risk of bias 63,67,81,82,88,[91][92][93]95,98 and 4 were at high risk of bias. 78,85,99,101 Common issues were identified in relation to patient selection, 63,67,78,82,85,88,89,[91][92][93]95,[97][98][99]101 interpretation of index test results, 63,67,78,81,82,85,88,[90][91][92][93][94][95][98][99][100]…”

Section: Risk Of Biasmentioning

confidence: 99%

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Laboratory‐based testing for hepatitis C infection using dried blood spot samples: A systematic review and meta‐analysis of diagnostic accuracy

Carty¹,

McCarthy

O’Neill

et al. 2021

Reviews in Medical Virology

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The use of dried blood spot (DBS) samples can facilitate the implementation of reflex testing by circumventing the need for centrifugation and freezing of venous blood samples. This systematic review assessed the accuracy of using DBS samples to diagnose chronic hepatitis C virus (HCV) infection. A comprehensive search was undertaken to identify articles published up to July 2020 evaluating the diagnostic accuracy of anti-HCV, HCV-RNA and HCV core antigen tests using DBS. Screening, data extraction, quality appraisal and Grading of Recommendations, Assessment, Development and Evaluations certainty of the evidence assessment were performed independently by two reviewers. Meta-analysis, meta-regression and sensitivity analyses were conducted. The evidence demonstrates that laboratorybased anti-HCV and HCV-RNA tests using DBS samples have high diagnostic accuracy. All comparisons were between DBS and venous samples. For the detection of anti-HCV, sensitivity was 95% (95% CI: 92%-97%) and specificity was 99% ([95% CI: 98%-99%]; n = 25; I 2 = 81%; moderate certainty). For the detection of HCV-RNA, the sensitivity was 95% (95% CI: 93%-97%) and specificity was 97% ([95% CI: 94%-98%]; n = 20; I 2 = 52%; moderate certainty). The sensitivity of HCV core antigen tests was 86% (95% CI: 79%-91%) and specificity was 98% ([95% CI: 94%-99%]; n = 5; I 2 = 37%; low certainty) compared with HCV-RNA (the gold standard for detecting chronic HCV). DBS samples could facilitate diagnosis of chronic HCV infection as the necessary sequential tests (anti-HCV and then HCV-RNA or HCV core antigen) can be undertaken using the same blood sample. This could reduce loss of patient follow-up and support international efforts towards HCV elimination in both high and low prevalence settings.

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Section: Resultsmentioning

confidence: 99%

Section: Study Characteristicsmentioning

confidence: 99%

Section: Risk Of Biasmentioning

confidence: 99%

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Laboratory‐based testing for hepatitis C infection using dried blood spot samples: A systematic review and meta‐analysis of diagnostic accuracy

Carty¹,

McCarthy

O’Neill

et al. 2021

Reviews in Medical Virology

View full text Add to dashboard Cite

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“…Since July 2018, our laboratory has incorporated reflex testing or one-step diagnosis in such a way that RNA is automatically tested after detecting HCV antibodies. As for diagnosis in DBS in drug treatment centres, RNA was determined directly given the high prevalence of infection in this group of patients, which is a technique that has been previously validated by our group (14) and has been part of the screening programme since January 2017. Direct RNA identification has been performed since August 2018 (15).…”

Section: Methodsmentioning

confidence: 99%

Hepatitis C diagnosis slowdown in high-prevalence groups and using decentralised diagnostic strategies during the COVID-19 pandemic

Morales-Arráez¹,

Benítez-Zafra²,

Díaz‐Flores³

et al. 2022

Rev Esp Enferm Dig

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D.M.A contributed to data collection, database generation, statistical analysis, writing and editing of the manuscript. F.B.Z assisted with data collection and database generation. F.D.F assisted with data collection from the laboratory and sample analysis. M.J.M.A, L.G.S and V.P.P. contributed to the screening and sample collection from the drug treatment centres. F.G.N collaborated with the analysis of the samples. M.H.G. contributed to the project design, coordination and final review and manuscript approval.

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“…According to the World Health Organization (WHO), approximately 3% worldwide are infected by hepatitis C virus (HCV), and it is annually estimated 3–4 million new infections and at least 150 million chronic carriers. Recently, the COVID-19 outbreak has increased even more mortality by HCV complications [ 2 ], even though the discovery of potent antivirals has considerably increased the chances of cure [ 3 ]. WHO plans to eradicate HCV by 2030 [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

An ultrasensitive electrochemical immunosensor for hepatitis C antibodies based on one-step-eletrosynthetized polypyrrole–graphene nanocomposite

et al. 2022

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An ultrasensitive label-free electrochemical immunosensor was developed for hepatitis C antibodies (anti-HCV). Worldwide, it is estimated 71 million people have HCV infection in a chronic stage that may lead to cirrose and cancer. To achieve HCV elimination, health programs should include screening testing based on anti-HCV detection allowing the early-stage treatment. The immunosensor was based on a graphene oxide-polypyrrole (PPy–GO) film one-step electropolymerized on the electrode surface. Ultrasensitive anti-HCV detection was ensured by HCV antigen conjugated to biotin that was immobilized in a great amount on streptavidin-coated nanostructured surface. Analytical responses were obtained by anodic peaks from the square wave voltammetry in the presence of ferrocyanide/ferricyanide as a redox probe. This immunosensor exhibited a linear range from 2 to 14 ng mL −1 of anti-HCV and a limit of detection in the clinical range (1.63 ng mL −1 ). Furthermore, the immunosensor presented an efficient performance for the determination of anti-HCV in spiked serum samples, becoming this developed nanosensor as potential tools for early HCV diagnosis and screening. Supplementary Information The online version contains supplementary material available at 10.1007/s10853-022-06992-5.

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Diagnostic test accuracy of the cobas 6800 system for detection of hepatitis C virus viraemia levels from dried blood spots

Cited by 5 publications

References 27 publications

Laboratory‐based testing for hepatitis C infection using dried blood spot samples: A systematic review and meta‐analysis of diagnostic accuracy

Laboratory‐based testing for hepatitis C infection using dried blood spot samples: A systematic review and meta‐analysis of diagnostic accuracy

Hepatitis C diagnosis slowdown in high-prevalence groups and using decentralised diagnostic strategies during the COVID-19 pandemic

An ultrasensitive electrochemical immunosensor for hepatitis C antibodies based on one-step-eletrosynthetized polypyrrole–graphene nanocomposite

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