Diagnostic Techniques in Veterinary Dermatology

Neuber, Ariane; Nuttall, Tim

doi:10.1002/9781119233053

Cited by 7 publications

(13 citation statements)

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“…Positive reactions to house dust mites possibly caused by cross-reactivity of storage mites, as previously described, cannot be ruled out [ 23 ]. Another limitation of this study was the concentration of D. pteronyssinus allergenic extract, which was prepared at the lower limit of an optimal concentration that was later revised [ 6 ]. This may have caused false-negative reactions of IDT to D. pteronyssinus and consequently may have affected the findings on atopic dermatitis development influenced by living in or outside of Bangkok.…”

Section: Discussionmentioning

confidence: 99%

“…The concentrated extracts were purchased either from ALK Abello (Port Washington, NY, 11050) or Siriraj Medical Hospital, Faculty of Medicine, Mahidol University (Bangkok, Thailand). The diluted allergenic extracts were prepared according to the manufacturer’s recommendation or as previously described [ 5 , 6 , 8 ] as follows: D. farinae (100 PNU/mL), D. pteronyssinus (100 PNU/mL), mixed mites (100 PNU/mL prepared from D. farinae 50 PNU/mL plus D. pteronyssinus 50 PNU/mL), and house dust (1000 PNU/mL). The extracts were kept below 7°C and used within 2 months.…”

Section: Methodsmentioning

confidence: 99%

“…Allergen selection for IDTs varies depending on regional prevalence [ 4 ]. Common mite allergens used for IDTs include single source extracts obtained from Dermatophagoides farinae or Dermatophagoides pteronyssinus or multisource extracts from multimite species (mixed mites), as well as a combination of multimite species and proteins from feces and shed skin (house dust) [ 5 , 6 ]. The relationship between IDT results for various mite allergens remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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Comparing the results of intradermal skin tests for four dust mite allergens in dogs with atopic dermatitis in Thailand

et al. 2020

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Background and Aim: Hypersensitivity to house dust mites is a common cause of atopic dermatitis in dogs. The intradermal test (IDT) identifies allergens to be included in allergen-specific immunotherapy. Common mite allergens used for IDT include single source extracts obtained from Dermatophagoides farinae or Dermatophagoides pteronyssinus or multisource extracts from multimite species (mixed mites), as well as a combination of multimite species and proteins from feces and shed skin (house dust). The objectives of the present study were to evaluate the prevalence of mite sensitivity in dogs diagnosed with atopic dermatitis in different Thailand provinces and to determine if positive test results to mite allergens aligned. Materials and Methods: Eighty-two dogs (median age [range]: 5 years [11 months-11 years]; 51 males and 31 females) diagnosed with atopic dermatitis underwent IDTs with four different mite-related allergens (D. farinae, D. pteronyssinus, mixed mites, and house dust). The skin reactions were reported on a scale of 0-4 and the reactions 2+ were considered clinically relevant. The relationship between IDT results from different allergens was determined using Pearson's correlation coefficient (r). Fisher's exact test was used to compare IDT results for different mite allergens as well as for dogs residing in Bangkok versus other provinces in Thailand. Results: The prevalence (95% confidence interval [CI]) of positive IDT results for D. farinae, D. pteronyssinus, mixed mites, and house dust in dogs with atopic dermatitis was 64.63% (52.30-74.88), 58.54% (47.12-69.32), 47.56% (36.41-58.89), and 35.37% (25.12-46.70), respectively. A moderate correlation was found in IDT results between D. pteronyssinus and house dust (r=0.514), between D. pteronyssinus and D. farinae (r=0.426), and between D. farinae and mixed mites (r=0.423). The prevalence of dogs with positive IDT results for mite allergens with mono-sensitization, bi-sensitization, tri-sensitization, and quadru-sensitization did not differ significantly between dogs residing in Bangkok (11.63%, 18.60%, 25.58%, and 16.28%) and dogs residing in other provinces (12.82%, 30.77%, 35.90%, and 10.26%). The overall sensitivity (95% CI) and specificity (95% CI) of the mixed mites test associated with atopic dermatitis in dogs were 60.32% (47.20-72.40%) and 94.70% (74.00-99.90%), respectively. The overall sensitivity (95% CI) and specificity (95% CI) of the house dust test associated with atopic dermatitis in dogs were 42.90% (30.50-56.00%) and 89.50% (66.90-98.70%), respectively. Conclusion: House dust mites are an important source of allergens for dogs with atopic dermatitis. In the present study, no significant difference in the prevalence of atopic dermatitis was found in dogs living in the urban area compared with dogs living in the countryside. Application of multisource extracts from mites for IDT revealed a higher reaction to mixed mites than that of house dust.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparing the results of intradermal skin tests for four dust mite allergens in dogs with atopic dermatitis in Thailand

et al. 2020

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“…Para un diagnóstico definitivo de dermatofitosis, las hifas septadas y/o artroconidias se deben detectar en la superficie de estos corneocitos (Gross y col., 2005;Albanese, 2017). Pueden observarse hifas y esporas de color purpura o azul con una tinción Diff Quick (Neuber y Nuttall, 2017). En el Perú aún no se han reportado estudios sobre el uso de la citopatología en el diagnóstico de dermatofitosis.…”

Section: Auspicio Y Apoyo Financierounclassified

“…Proporción de concordancia entre la técnica de cultivo micológico y la citología como diagnóstico de dermatofitosis en cuyes. La técnica de citopatología es frecuentemente usada para determinar la presencia de agentes etiológicos en lesiones dermatológicas, debido a que es fácil de realizar, rápida, mínimamente invasiva; además no implica un costo muy alto (Neuber y Nuttall, 2017); sin embargo, no es ampliamente utilizada para este fin. Dentro de esta técnica el mejor método para la toma de muestra es el de la cinta adhesiva.…”

Section: Total De Animalesunclassified

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Pilot study of dogs with suppurative and non-suppurative Malassezia otitis: A case series

Rodrigues

Vandenabeele

2021

BMC Vet Res

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Background Rarely, Malassezia otitis presents as a painful, erosive otitis with an otic discharge containing Malassezia and neutrophils on cytology. There are no published reports of this type of suppurative Malassezia otitis (SMO). The role of Malassezia hypersensitivity in otitis is still unknown, and no association has been demonstrated with SMO. We compared Malassezia IgE levels, intradermal test and histology changes in SMO dogs with the more conventional Malassezia otitis (MO) presentation. Results Three dogs (case 1, case 2 and case 3) were diagnosed with SMO, one dog (case 4) was diagnosed with unilateral MO and unilateral SMO, and one dog (case 5) was diagnosed with MO. Only one case (case 4) with SMO/MO had a positive Intradermal Allergy Test (IDAT) and elevated IgE levels for Malassezia. Histopathology findings from SMO revealed: interface dermatitis (case 1 and 3), lymphocytic dermatitis (case 2) and chronic hyperplastic eosinophilic and lymphoplasmacytic dermatitis (case 4). Histopathology findings from MO showed perivascular dermatitis (case 4 and 5). All the cases were treated successfully. Conclusions SMO presents with a distinct clinical phenotype in comparison with conventional MO. No consistent aetiology could be isolated. In these clinical cases it is possible that previous treatments could have influenced the results. More research is needed to understand the possible aetiologies and the pathogenesis of SMO.

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Diagnostic Techniques in Veterinary Dermatology

Cited by 7 publications

References 0 publications

Comparing the results of intradermal skin tests for four dust mite allergens in dogs with atopic dermatitis in Thailand

Comparing the results of intradermal skin tests for four dust mite allergens in dogs with atopic dermatitis in Thailand

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Pilot study of dogs with suppurative and non-suppurative Malassezia otitis: A case series

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