Diagnostic significance of nuclear DNA content and nuclear area in oral hyperplasia, dysplasia, and carcinoma

Kinoshita, Yukihiko; Inoue, Satoshi; Honma, Yoshiro; Shimura, Kaizou

doi:10.1016/0278-2391(92)90108-c

Cited by 12 publications

(7 citation statements)

References 20 publications

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“…Quantitative measures of normal oral epithelium and oral SCC find almost a doubling in DNA content in SCC when compared to normal tissue. 32 Our results are similar to the 22 and 69 cm −1 for normal and dysplastic epithelium, respectively, extracted from cervical tissue confocal images 28 and the 13 and 142 cm −1 for normal and dysplastic epithelium predicted by our finite difference time domain (FDTD) algorithm, which estimated the volume fraction of nuclei and average nuclear size from cervical confocal images. 33 We also observed higher scattering in normal oral mucosa than was found in cervical epithelium, which we attribute to higher levels of keratin in the oral cytoplasm.…”

Section: Discussionsupporting

confidence: 81%

Detection and diagnosis of oral neoplasia with an optical coherence microscope

et al. 2004

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The use of high resolution, in vivo optical imaging may offer a clinically useful adjunct to standard histopathologic techniques. A pilot study was performed to investigate the diagnostic capabilities of optical coherence microscopy (OCM) to discriminate between normal and abnormal oral tissue. Our objective is to determine whether OCM, a technique combining the subcellular resolution of confocal microscopy with the coherence gating and heterodyne detection of optical coherence tomography, has the same ability as confocal microscopy to detect morphological changes present in precancers of the epithelium while providing superior penetration depths. We report our results using OCM to characterize the features of normal and neoplastic oral mucosa excised from 13 subjects. Specifically, we use optical coherence and confocal microscopic images obtained from human oral biopsy specimens at various depths from the mucosal surface to examine the optical properties that distinguish normal and neoplastic oral mucosa. An analysis of penetration depths achieved by the OCM and its associated confocal arm found that the OCM consistently imaged more deeply. Extraction of scattering coefficients from reflected nuclear intensity is successful in nonhyperkeratotic layers and shows differentiation between scattering properties of normal and dysplastic epithelium and invasive cancer.

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Section: Discussionsupporting

confidence: 81%

Detection and diagnosis of oral neoplasia with an optical coherence microscope

et al. 2004

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“…In the present study, computerized image analysis of nuclear morphometric features like area, minimum diameter, and perimeter showed an increase in histological sections of OSCC as compared to normal buccal mucosa (control) in concordance with the study by van der Wal et al and Kinoshita et al [ 9 , 15 ].…”

Section: Discussionsupporting

confidence: 92%

“…An inverse relation between increased nuclear perimeter and loss of differentiation was noted in our study where we found a progressive increase in nuclear perimeter at invasive front from well differentiated to poorly differentiated squamous cell carcinoma. This is in agreement with the findings of Kinoshita et al who in a combined study of 130 cases of precancerous and cancerous lesions found a progressive increase in nuclear DNA content, nuclear area, and perimeter at tumor proper that related directly to the proliferation of cells [ 15 , 20 ].…”

Section: Discussionsupporting

confidence: 92%

“…Among other histological parameters, we noted an inverse correlation between degree of mitosis at invasive front and rate of recurrence and event of death. Bryne et al stated that low mitotic count may be seen at the invasive front of the tumor as compared to solid areas of the tumor with high dissociation of cells in the most invasive parts of tumor [ 15 , 16 ]. In addition, Bryne et al (in the same study) also assessed the prognosis of OSCC patients by separately grading the ITF with “inclusion” and subsequently by “omission” of the parameter of “mitotic count.” The prognostic value still remained highly significant and reproducibility improved when the parameter of “mitotic count” was eliminated from IFG [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

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Prognostic Efficacy of Nuclear Morphometry at Invasive Front of Oral Squamous Cell Carcinoma: An Image Analysis Microscopic Study

Vedam

Boaz

Natarajan

2014

Analytical Cellular Pathology

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Background. Oral cancer is revisited on a pathologist perspective with advanced imaging technique. Objective. The present study assessed the new malignancy grading system at tumor proper (TP) and Bryne's grading system at invasive tumor front (ITF), morphometric features using software, to clarify their associations with prognosis of oral cancers. Methods. Histologically confirmed oral squamous cell carcinoma (OSCC) with 5-year follow-up was assessed morphometrically using image analysis at TP and ITF, correlated with the prognosis of patient. Results. On comparison of grading systems, a moderate agreement between both (Bryne and Anneroth) was seen. Among all histological parameters, we noted inverse correlation between degree of mitosis at invasive front and decrease in lymphoplasmacytic infiltrate at ITF with increase rate of recurrence and event of death. An increase in nuclear area, diameter, and perimeter along with decrease circularity in advancing OSCC was seen. Correlation of parameters showed higher values for maximum nuclear diameter, perimeter, and circularity at TP and ITF with recurrence. Conclusion. This study, while limited in sample size, concluded that a combined assessment of clinical TNM staging, histopathological grading system {excluding the parameter “mitotic activity” (due to its inverse relation)}, and nuclear morphometry at the ITF are better prognosticators. This combination proved to be an accurate predictive factor in eliciting the varied molecular characteristics of tumor heterogeneity.

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“… 3 – 5 One reason for the poor prognosis of patients with cancer of the oral cavity is that the mitotic index of these cells is low. 6 Because of this low mitotic index, cells have a reduced ability to undergo apoptosis – the regular cell death process that should ensure an elimination of cells with genetic alterations. 2 Inappropriate apoptosis is a factor in many human conditions including many types of cancer.…”

Section: Introductionmentioning

confidence: 99%

Impact of combined treatment with nimesulide and cisplatin on oral carcinoma cells

Barać¹,

Mitulović

Hallström

et al. 2017

OTT

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BackgroundDespite significant advances in diagnosis and therapy, the rate of survival of patients with oral cancers still remains poor as an appropriate treatment has not been found yet, due to side effects of chemo/radiotherapy.AimThis study aimed to identify molecular mechanisms of cell death of oral cancer cells caused by treatment with a nonselective Cox-2 inhibitor in combination with a low-dose chemotherapeutic drug.MethodsSquamous cell carcinoma (SCC) cells SCC9 and SCC25 were subjected to mono- and combination therapy with nimesulide and cisplatin. Fluorescence-activated cell sorting (FACS), immunohistochemistry, high-pressure liquid chromatography (HPLC), microarray gene chips, and isobaric tags for a relative and absolute quantitation (iTRAQ) system were used.ResultsIncreased numbers of apoptotic and necrotic SCC9/SCC25 cells were detected after combined exposure. ATP levels and the energy charge of SCC9 cells were significantly decreased after both individual and combined treatment. We detected and quantified a responsible gene, keratin 6a, and 540 relevant proteins. In SCC25 cells, ATP levels significantly decreased only after combination therapy. After combined treatment of SCC9 cells, significant upregulation of Histon-H2A/H2B/H4 was found, with a local discovery false rate of 0.003 for Histon-H2A and 0.0027 for Histon-H2B, respectively.ConclusionCompared to the single-drug treatment, combined treatment of the oral cancer cells with nimesulide and cisplatin increases and induces necrosis and apoptosis through different pathways. A significant effect of the cytoplasmic increase was also observed in histones of cell lines SCC9 and SCC25 that were previously treated with combined nimesulide and cisplatin therapy.

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Diagnostic significance of nuclear DNA content and nuclear area in oral hyperplasia, dysplasia, and carcinoma

Cited by 12 publications

References 20 publications

Detection and diagnosis of oral neoplasia with an optical coherence microscope

Detection and diagnosis of oral neoplasia with an optical coherence microscope

Prognostic Efficacy of Nuclear Morphometry at Invasive Front of Oral Squamous Cell Carcinoma: An Image Analysis Microscopic Study

Impact of combined treatment with nimesulide and cisplatin on oral carcinoma cells

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