Diagnostic Performance of a Reverse Transcription-Polymerase Chain Reaction Test for Porcine Reproductive and Respiratory Syndrome Virus

Characterization of the carrier state in porcine reproductive and respiratory syndrome virus infection

“…This statement, however, must be tempered with the observation that PCR protocols among laboratories are non-uniform and in the midst of rapid evolution as new technology emerges. In some cases, commercially-available PCR-based assays have demonstrated less than adequate performance (Wagstrom et al, 2000). The nested PCR procedure used in this study, although labor-intensive, was validated and shown to be analytically highly sensitive .…”

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): A Ring Test Performed in Germany to Assess RT‐PCR Detection Methods

“…As reverse‐transcriptase polymerase chain reaction (RT‐PCR) does not need virus replication it can be performed even with non‐infectious material and within few hours. RT‐PCR‐based assays are believed to be highly sensitive and highly specific, although Wagstrom et al. (2000) have shown that this is not always the case for PRRSV.…”

Opinion of the Scientific Panel on Animal Health and Welfare (AHAW) on a request from the Commission related to the probability of transmission of Porcine Reproductive and Respiratory Syndrome virus (PRRSv) to naive pigs via fresh meat

“…In addition, RT-PCR can also be used for the detection of PRRSv in samples like semen, fetal tissues, thoracic fluids or faeces because these samples are difficult to analyze by traditional methods (Benson et al, 2002;Yoon et al, 2003). However, it should be recognised that both false negative as positive RT-PCR results may occur (Wagstrom et al, 2000).…”

“…Some RT-PCR assays are based on a "nested" principle (RT-nPCR) for added sensitivity. More recently, automated fluorogenic RT-PCR-based tests, such as the TaqMan™ PCR (Molitor et al, 1997b;Wagstrom et al, 2000;Bierk et al, 2000;Egli et al, 2001;Balasuriya et al, 2002), Light-Cycler™ PCR (van Rijn et al, 2004) or "Molecular Beacon" PCR (Carlson et al, 2002) have been developed to improve the quality of conventional RT-PCR tests for detecting PRRSv in clinical specimens.…”