Diagnostic model of saliva peptide finger print analysis of oral squamous cell carcinoma patients using weak cation exchange magnetic beads

Jiang, Wei-Peng; Wang, Zhen; Xu, Li; Xin, Peng; Chen, Feng

doi:10.1042/bsr20150023

Cited by 18 publications

(15 citation statements)

References 65 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, a study has shown that histatin‐1 is highly expressed in melanoma cell lines and clinical samples, implicating it as a potential prognostic marker for this malignancy (Yaguchi & Kawakami, ). Likewise, a subsequent study correlated the amount of salivary histatin‐3 with the progression of oral squamous cell carcinoma in patients, using MALDI‐TOF‐MS combined with magnetic beads (Jiang, Wang, Xu, Peng, & Chen, ). These studies are relevant in the context of the searching for new biomarkers that allow an early and reliable diagnosis of different types of cancer.…”

Section: Histatins Cell Migration and Oral Wound Healingmentioning

confidence: 99%

Histatins, wound healing, and cell migration

et al. 2018

View full text Add to dashboard Cite

Wounds in the oral mucosa heal faster and more efficiently than those in the skin, although the mechanisms underlying these differences are not completely clear. In the last 10 years, a group of salivary peptides, the histatins, has gained attention on behalf of their ability to improve several phases of the wound-healing process. In addition to their roles as anti-microbial agents and in enamel maintenance, histatins elicit other biological effects, namely by promoting the migration of different cell types contained in the oral mucosa and in non-oral tissues. Histatins, and specifically histatin-1, promote cell adhesion and migration in oral keratinocytes, gingival and dermal fibroblasts, non-oral epithelial cells, and endothelial cells. This is particularly relevant, as histatin-1 promotes the re-epithelialization phase and the angiogenic responses by increasing epithelial and endothelial cell migration. Although the molecular mechanisms associated with histatin-dependent cell migration remain poorly understood, recent studies have pointed to the control of signaling endosomes and the balance of small GTPases. This review aimed to update the literature on the effects of histatins in cell migration, with a focus on wound healing. We will also discuss the consequences that this increasing field will have in disease and therapy design.

show abstract

Section: Histatins Cell Migration and Oral Wound Healingmentioning

confidence: 99%

Histatins, wound healing, and cell migration

et al. 2018

View full text Add to dashboard Cite

show abstract

“…MALDI-TOF MS is a valuable tool for the profiling of biological samples with relatively low abundance that is fast, highly sensitive, and has a high throughput [20]. In addition, the use of MALDI-TOF MS combined with magnetic beads is a new method that has been used to identify cancer and other diseases [21][22][23][24][25]. This method is also suitable for detection of serum protein biomarkers in liver transplant patients.…”

Section: Discussionmentioning

confidence: 99%

Serum proteomic predicts effectiveness and reveals potential biomarkers for complications in liver transplant patients

Wang

Liu

et al. 2020

Aging

View full text Add to dashboard Cite

Sophisticated postoperative complications limit the long-term clinical success of liver transplantation. Hence, early identification of biomarkers is essential for graft and patient survival. High-throughput serum proteomics technologies provide an opportunity to identify diagnostic and prognostic biomarkers. This study is aimed to identify serum diagnosis biomarkers for complications and monitor effectiveness. Serum samples from 10 paired pre-and post-liver transplant patients, 10 acute rejection (AR) patients, 9 ischemic-type biliary lesion (ITBL) patients, and 10 healthy controls were screened using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to explore divergence in polypeptide. Then, we used ELISA and western blot analysis to validate the expression of these potential biomarkers, and studied the correlation of proteomic profiles with clinical parameters. ACLY, FGA, and APOA1 were significantly lower in pre-operative patients compared with healthy controls, and these patients had modest recovery after transplantation. Downregulation of both, ACLY and FGA, was also observed in AR and ITBL patients. Furthermore, bioinformatics analysis was performed and the results suggested that the identified proteins were involved in glucolipid metabolism and the clotting cascade. Together, these findings suggest that ACLY, FGA, and APOA1 could be novel non-invasive and early biomarkers to detect complications and predict effectiveness of liver transplantation.

show abstract

“…The saliva peptide finger print technique is a useful tool for salivary proteomics analysis and can predict potential biomarkers valuable for cancer diagnosis [ 124 ]. A study utilized matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry (MALDI-TOF–MS) for analyzing the expression spectrum of salivary peptides in 40 OSCC patients and 23 normal controls [ 125 ]. Nanomaterial-based magnetic beads were used for selective enrichment of low-molecular-mass peptides.…”

Section: Nanotechnology-based Detection and Diagnostic Methodsmentioning

confidence: 99%

“…As a result, the mass peaks of 1285.6 and 1432.2 Da, which were both identified as histatin-3, were correlated with OSCC progression. This study introduced a novel high-throughput, non-invasive strategy for valuable oral cancer biomarkers screening [ 125 ]. The specific advantages of magnetic beads constructed on nanomaterial over other types of separation beads have not yet been illustrated.…”

Section: Nanotechnology-based Detection and Diagnostic Methodsmentioning

confidence: 99%

Nanotechnology: a promising method for oral cancer detection and diagnosis

et al. 2018

View full text Add to dashboard Cite

Oral cancer is a common and aggressive cancer with high morbidity, mortality, and recurrence rate globally. Early detection is of utmost importance for cancer prevention and disease management. Currently, tissue biopsy remains the gold standard for oral cancer diagnosis, but it is invasive, which may cause patient discomfort. The application of traditional noninvasive methods-such as vital staining, exfoliative cytology, and molecular imaging-is limited by insufficient sensitivity and specificity. Thus, there is an urgent need for exploring noninvasive, highly sensitive, and specific diagnostic techniques. Nano detection systems are known as new emerging noninvasive strategies that bring the detection sensitivity of biomarkers to nano-scale. Moreover, compared to current imaging contrast agents, nanoparticles are more biocompatible, easier to synthesize, and able to target specific surface molecules. Nanoparticles generate localized surface plasmon resonances at near-infrared wavelengths, providing higher image contrast and resolution. Therefore, using nano-based techniques can help clinicians to detect and better monitor diseases during different phases of oral malignancy. Here, we review the progress of nanotechnology-based methods in oral cancer detection and diagnosis.

show abstract

Diagnostic model of saliva peptide finger print analysis of oral squamous cell carcinoma patients using weak cation exchange magnetic beads

Abstract: Whole saliva (WS) was used for diagnosis of oral squamous cell carcinoma (OSCC); two polypeptides may be used for OSCC diagnosis.

Cited by 18 publications

References 65 publications

Histatins, wound healing, and cell migration

Histatins, wound healing, and cell migration

Serum proteomic predicts effectiveness and reveals potential biomarkers for complications in liver transplant patients

Nanotechnology: a promising method for oral cancer detection and diagnosis

Contact Info

Product

Resources

About