Diagnostic DNA amplification no respite for the elusive parasite

Bruijn, M.H.L. de

doi:10.1016/0169-4758(88)90028-2

Cited by 22 publications

(2 citation statements)

References 16 publications

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“…oon after the first publications on the in vitro amplification of DNA by PCR (1,2), it was predicted that this new technology would lead to a breakthrough in molecular parasitology and in the diagnosis of parasitic infections (3,4). Until that time, specific DNA probes had been used in basic research and also in diagnostic applications despite being hampered by the limited sensitivity of such direct hybridization assays without a preceding amplification step.…”

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confidence: 99%

Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections

2014

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SUMMARY Over the past few decades, nucleic acid-based methods have been developed for the diagnosis of intestinal parasitic infections. Advantages of nucleic acid-based methods are numerous; typically, these include increased sensitivity and specificity and simpler standardization of diagnostic procedures. DNA samples can also be stored and used for genetic characterization and molecular typing, providing a valuable tool for surveys and surveillance studies. A variety of technologies have been applied, and some specific and general pitfalls and limitations have been identified. This review provides an overview of the multitude of methods that have been reported for the detection of intestinal parasites and offers some guidance in applying these methods in the clinical laboratory and in epidemiological studies.

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confidence: 99%

Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections

2014

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“…Such methods have detected Rickettsia typhi (Webb et al 1990) and Borrelia burgdorferi (Persing et al 1990) in arthropods. DNA purification has been reported to be the most efficient method of sample preparation (de Bruijn 1988), but sensitive techniques have been reported in the absence of DNA extraction (Fekete et al 1990;Webb et al 1990). Previously, Anaplasma marginale has been detected in cattle and ticks with a DNA probe derived from a gene encoding the 13 subunit for the Major Surface Protein 1 (MSP 113) of A. marginale, Florida isolate (Goff et al 1988).…”

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confidence: 98%