Diagnostic and Therapeutic Significance of the Oxidative Stress Parameters in Children

Kratak sadr`aj: Ispitivan je uticaj intracere bro ventrikularne administracije angiotenzina II i kaptoprila na napetost i oksidativni stres u mozgu. Za procenjivanje po na{anja bliskog anksioznosti kori{}en je test uzdignutog lavirinta u obliku znaka plus, dok je biohemijska analiza obu hvatila odre|ivanje nekih enzima antioksidantne odbrane kao {to su superoksid-dismutaza i glutation-peroksidaza i pro izvoda lipidne peroksidacije (malondialdehid). Na{i re zultati predstavljaju jo{ jedan dokaz da angiotenzin II iza ziva posledice nalik na anksioznost i povi{en prooksidantni sta tus.[tavi{e, blokiranje angiotenzina II davanjem inhibitora enzima koji konvertuje angiotenzin (kaptoprila) delo valo je kao anksiolitik i dovelo do sni`enja statusa oksi dativnog stresa. Pored toga, otkrivena je zna~ajna korelacija izme |u vremena koje su pacovi proveli u otvorenim »ruka ma« lavirinta i markera oksidativnog stresa. Ovo mo`da uka zuje na va`nost pokretanja nekih va`nih terapijskih pita nja koja se ti~u anksioliti~kog dejstva nekih inhibitora enzi ma koji konvertuju angiotenzin a koji se pre svega koriste za hipertenziju, kakav je kaptopril. Tako|e, sva je prilika da oksi dativni stres u tome igra va`nu ulogu.

Section: Discussionsupporting

confidence: 90%

Inhibition of Central Angiotensin Converting Enzyme Exerts Anxiolytic Effects by Decreasing Brain Oxidative Stress

Ciobîcă¹,

Hrițcu²,

Năstasă³

et al. 2011

“…Plasma GR and GPx activities were in our study evidently related to the maturation stage of SC, thus their synergistic reduction could also be an epiphenomenon of the disease severity. Physical inactivity, for example, which often accompanies the greater visual disability, has been shown to decrease both kidney and lens GPx and GR in experimental models, as well as erythrocytes GR in healthy elderly people (29)(30)(31)(32)(33)(34). Plasma GPx and GR were in our study well correlated with correspondent lens enzymes in either stage of SC, suggesting that they may serve as lens surrogate markers.…”

Section: Discussionsupporting

confidence: 61%

The Impact of Senile Cataract Maturity on Blood Oxidative Stress Markers and Glutathione-Dependent Antioxidants: Relations with Lens Variables

Mirić¹,

Kisic²,

Zoric³

et al. 2012

The Impact of Senile Cataract Maturity on Blood Oxidative Stress Markers and Glutathione-Dependent Antioxidants: Relations with Lens VariablesOxidative stress is implicated in senile cataract (SC) genesis, although the impact of SC maturity on blood oxidative stress markers is unclear. Total hydroperoxides, malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S-transferase (GST) were measured in the blood and lens samples of patients having either immature (n=31) or mature SC (n=50), and in 22 blood samples from noncataract controls. Compared to controls, SC patients had higher plasma MDA and serum GST, and decreased plasma GR and GSH levels. Plasma GPx as well as hydroperoxides differed from control values only in patients with mature SC. The multivariate logistic regression analysis showed that the fall of plasma GR activity (OR 5.14; CI 1.82-14.51;p=0.0020), as well as serum GST activity (OR 3.84; CI 1.36-10.83;p=0.0108) were independently associated with the maturity of SC. Lens hydroperoxides, MDA and GST, showed no correlation with correspondent blood values, in contrast to GPx (r=0.715; p<0.001) and GR (r=0.703; p<0.001). This study showed that the severity of SC is associated with increased systemic oxidative stress, which could be due to the fall of GSH-dependent antioxidant enzymes activities.

“…On the other hand, higher GPX activity in the elderly subjects com pared with the younger ones may represent an adaptive response to intensified oxi dative stress like in the case of CAT. The total antioxidant enzyme capa city of erythrocytes was in the young and old subjects alike, indicating the existence of compen satory changes in antioxidant enzyme activities (29,30).…”

Section: Discussionmentioning

confidence: 99%

Age-Related Blood Antioxidant Capacity in Men and Women

Hϋbner-Woźniak¹,

Okęcka-Szymańska²,

Stupnicki³

et al. 2011

Kratak sadr`aj: Cilj studije bio je da se odredi antioksidantni kapacitet u krvi kod mu{karaca i `ena u odnosu na njihovu starost. Ispitano je 19 mu{karaca (YM) i 19 `ena (YW) starosti 25-32 godine, kao i 11 mu{karaca (OM) i 11 ena (OW) starosti 63-71 godine, koji su svi bili neaktivni. Odre|eni su slede}i faktori: aktivnost superoksid dismutaze (SOD), glutation-reduktaze (GR), glutation-peroksidaze (GPX), katalaze (CAT), ukupni antioksidantni status (TAS) u eritrocitima, kao i retinol, alfa-tokoferol, mokra}na kiseli na i ukupne koncentracije proteina u plazmi. Izra~unat je zbir standardizovanih aktivnosti antioksidantnih enzima ka ko bi se uporedile promene u ukupnom kapacitetu anti oksi dantne odbrane u eritrocitima vezane za starosno doba. Nisu uo~e -ne zna~ajne promene vezane za starosno doba u aktivnosti SOD; srednja aktivnost CAT bila je vi{a kod starijih `ena i mu{karaca nego kod mla|ih ispitanika. Srednja aktivnost GPX bila je vi{a a aktivnost GR ni`a kod starijih ispitanika u pore|enju s mla|ima. Izra ~u nati ukupni antioksidantni enzimski kapacitet u eritrocitima dao je sli~ne vrednosti kod mla|ih i kod starijih ispitanika. Nisu uo~ene zna~ajne razlike u koncentracijama retinola i alfa-tokoferola u plazmi u odnosu na pol ili starosno doba. Ukupni nivo proteina u plazmi bio je zna~ajno ni`i kod mladih `ena i mu{karaca u pore|enju sa starijim ispitani cima. Zaklju~eno je da se ukupni enzimski antio ksidantni kapacitet u eritrocitima ne menja s godinama. Dobijeni rezultati jasno pokazuju da u procesu starenja u~estvuje vi{e faktora.