Diagnostic and prognostic significance of serum MicroRNAs in colorectal cancer

Menéndez, Pablo; Villarejo, Pedro; Padilla, David; Menéndez, José María; Montes, J. A. Rodríguez

doi:10.1002/jso.23245

Cited by 29 publications

(18 citation statements)

References 33 publications

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“…These findings suggest that miRNAs may play a role in promoting or inhibiting tumour development. [19][20][21][22] Tang et al 23 investigated miRNA expression in adipose stromal cells and found that miR-22 played an important role in regulating the development of these cells. miR-22 was aberrantly expressed in many tumour tissues, including breast cancer, colorectal cancer and cervical carcinoma.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-22 expression in hepatocellular carcinoma and its correlation with ezrin protein

Zhou

Yang-de

2013

J Int Med Res

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Objective: Expression of microRNA-22 (miR-22) and ezrin protein (a membrane-cytoskeleton linking protein) in hepatocellular carcinoma (HCC) was investigated. Methods: Specimens of HCC and paracancerous tissue (control; $5 cm away from tumour tissue) were collected from 192 patients. miR-22 expression was detected by real-time polymerase chain reaction; ezrin protein expression in tumour tissue was assessed immunohistochemically. Associations between miR-22 expression and clinicopathological features of HCC and ezrin expression were analysed. Results: miR-22 expression was lower in HCC tissue than in paracancerous tissue samples (median relative expression 0.676 versus 1.000 for control tissue). Expression of miR-22 was significantly associated with histological differentiation (relative expression 0.431 for lower grades of differentiation versus 0.918 for higher grades), and was associated with lymphatic metastasis (relative expression 0.518 if metastasis was present, 0.919 if absent). Survival time was shorter in patients with low miR-22 expression than in those with high expression (31.0 AE 2.6 versus 52.2 AE 5.1 months). There was a significant negative correlation between the expression of miR-22 and that of ezrin. Conclusions: miR-22 is downregulated in HCC and its expression is associated with the differentiation, metastasis and prognosis of the carcinoma. Ezrin is a potential regulatory protein of miR-22.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-22 expression in hepatocellular carcinoma and its correlation with ezrin protein

Zhou

Yang-de

2013

J Int Med Res

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“…Specific miRNAs function as negative or positive regulators of genes involved the development of tumor types (He et al, 2004;Bartel., 2009), such as lung cancer (Du et al, 2012), colorectal cancer (Menéndez et al, 2013), breast cancer (Liu., 2009), and gastric cancer (Wu et al, 2014). Gastric cancer is a major malignant tumor type, representing the second most common cancer and the leading cause of cancer mortality worldwide.…”

Section: Macrophage-secreted Exosomes Delivering Mirna-21 Inhibitor Cmentioning

confidence: 99%

Macrophage-secreted Exosomes Delivering miRNA-21 Inhibitor can Regulate BGC-823 Cell Proliferation

Wang¹,

Wang²,

Chen³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Exosomes, membranous nanovesicles, naturally carry bio-macromolecules or miRNA and play impoetant roles in tumor pathogenesis. Here, we showed that macrophages cell-derived exosomes can function as vehicles to deliver exogenous miR-21 inhibitor into BGC-823 gastric cancer cells. Exosomes loaded with miR-21inhibitor significantly increased miR-21 levels in BGC-823, but miR-21inhibitor loaded in exosomes exerted an opposite effect. miRNA transfected with exosomes had less cellular toxicity to host cells compared to conventional transfection methods. The miR-21inhibitor loaded exosomes promoted the migration ability and reduced apoptosis of BGC-823 gastric cancer cells. These observations indicate that miR-21 acts as a tumor promoter by targeting the PDCD4 gene and preventing apoptosis of gastric cancer cells through inhibition of PDCD4 expression. Furthermore, exosome -mediated miR-21 inhibitor delivery resulted in functionally more efficient inhibition and less cellular toxicity compared to conventional transfection methods. Similar approaches could be useful in modification of target biomolecules in vitro and in vivo. These findings contribute to our understanding of the functions of miR-21 and exosomes as a carrier for therapy of gastric cancer.

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“…In a groundbreaking work, Mitchell et al [38] have demonstrated that circulating miRNAs are present in the human blood, where they are carried in complex with the chaperon argonaute 2 and/or lipoproteins or by microvesicles, conferring stability and protection against RNases [39]. Correlations exist between blood miRNA levels and pathologies, indicating that miRNAs are potential noninvasive biomarkers [40]. It is thus reasonable to hypothesize that miRNA seric or plasma expression is a most promising avenue to evaluate early risk and patient outcome, but also individual patient response to various treatments or surgical procedures.…”

Section: Rna-based Mechanismsmentioning

confidence: 99%

The Management of Cardiovascular Risk through Epigenetic Biomarkers

Metzinger

Franciscis

Serra

2017

BioMed Research International

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Epigenetic sciences study heritable changes in gene expression not related to changes in the genomic DNA sequence. The most important epigenetic mechanisms are DNA methylation, posttranslational histone modification, and gene regulation by noncoding RNAs, such as microRNAs (miRNAs) and long noncoding RNAs (lncRNAs). Cardiovascular diseases (CVD) are responsible for at least one-third of premature deaths worldwide and represent a heavy burden of healthcare expenditure. We will discuss in this review the most recent findings dealing with epigenetic alterations linked to cardiovascular physiopathology in patients. A particular focus will be put on the way these changes can be translated in the clinic, to develop innovative and groundbreaking biomarkers in CVD field.

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Diagnostic and prognostic significance of serum MicroRNAs in colorectal cancer

Cited by 29 publications

References 33 publications

MicroRNA-22 expression in hepatocellular carcinoma and its correlation with ezrin protein

MicroRNA-22 expression in hepatocellular carcinoma and its correlation with ezrin protein

Macrophage-secreted Exosomes Delivering miRNA-21 Inhibitor can Regulate BGC-823 Cell Proliferation

The Management of Cardiovascular Risk through Epigenetic Biomarkers

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