Diagnostic accuracy of proximal enamel subsurface demineralization and its relationship with calcium loss and lesion depth

Abstract

Objective: The aim of this study was to determine the relationship between the amount of calcium loss, lesion depth, and the accuracy of storage phosphor plate (SPP) and film radiographs for the detection of artificial proximal demineralization. Methods: Standard enamel windows of extracted premolars were exposed to a demineralizing solution for 60 h, 80 h, 100 h and 120 h. Solutions were analysed for calcium concentration by atomic absorption spectrometer and the lesion depths were calculated by a specific formula. All teeth were radiographed with SPPs and F-speed films before and after acid application. Images were evaluated by five observers. Stereomicroscopic and scanning electron microscopic (SEM) observations were carried out to visualize enamel surfaces after acid exposure. Receiver operating characteristic analysis was used for diagnostic accuracy (A z ). A z s were compared with factorial analysis of variance and t-tests. The relationship between A z s and lesion depths was determined with Pearson's correlation test. Results: Strong positive correlation was found between A z s of both radiographic methods and lesion depths. No difference was found between the A z s of two radiographic systems for any of the demineralization durations (p . 0.05). Pair-wise comparisons revealed no significant difference in A z s of SPPs (p . 0.05), while significant differences were obtained for the A z s of films for different demineralization periods (p , 0.05). Stereomicroscopic and SEM observations confirmed demineralizations from superficial to deeper layers of enamel. Conclusion: Subsurface enamel demineralization was not accurately detectable with either storage phosphor plates or F-speed films. The amount of calcium loss and the depth of demineralization have a strong relationship with diagnostic accuracy with a significant effect particularly on F-speed films.

Intraoralversusextraoral bitewing radiography in detection of enamel proximal caries: anex vivostudy

“…17 We did not use the canine as its proximal caries is usually detected by periapical radiography. 18,19 Similarly, Kamburoglu et al 9 Similar to Onem et al, 24 we used sound human premolars and molars teeth to precisely induce artificial proximal caries with different depth so that we can assess the ability of radiographic grading of caries and accuracy of incipient proximal caries detection. On the other hand, Castro et al 25 Dry human skulls and mandibles were used, and the teeth were adapted with their roots into the empty sockets to simulate a real dental arch as much as possible.…”

Effects of different orthodontic primers on enamel demineralization around orthodontic brackets

“…Die alten Lösungen wurden jeweils -im Hinblick auf die nachfolgende Quantifizierung ihrer Kalziumkonzentrationen per Atomabsorptionsspektralphotometer -in ihren Röhrchen aufbewahrt [19,28]. Die Probekörper in den Lösungen wurden in Polypropylenröhrchen gegeben (ein Probekörper pro Röhrchen), bei 37°C im Ofen gelagert, die Zähne nach 96 h aus der Lösung genommen und diese per Atomabsorptions-Spektroskopie auf ihren Kalziumionengehalt beurteilt.…”

“…At the end of the fourth day, each sample was taken out and placed into fresh buffer solution. The previous solutions were kept in their tubes to be subsequently tested for their calcium concentrations with an atomic absorption spectrophotometer [19,28]. The samples immersed in the solutions were placed in polypropylene tubes (one sample per sample per tube) and were stored in an oven at 37°C.…”

Knowledge of Appropriate Prescription of Dental Radiographs among Interns of Two Dental Institutes of Belagavi City: A Questionnaire Study

“…Radiographic detection of caries is primarily based on the fact that as the caries process proceeds, the mineral content of enamel and dentin decreases. 10 Wenzel 11 claimed that lesions confined to enamel may not be evident radiographically until approximately 30 to 40% demineralization occurs. Yang and Dutra 12 stated that 40 to 60% of tooth decalcification is required for a lesion to be seen radiographically.…”