Diagnostic accuracy of molecular testing with three molecular markers on thyroid fine‐needle aspiration cytology with abnormal category

Şeneldir, Hatice; Kır, Gözde; Soylemez, Tuce; Girgin, Rabia Burçin; Özbay, Nurver; Özen, Filiz; Ankaralı, Handan; Baş, Gürhan; Ali̇moğlu, Orhan

doi:10.1002/dc.24394

Cited by 9 publications

(7 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…29 In summary, LBC specimens from the thyroid and lymph nodes demonstrated several recurrent mutations using a customized cancer gene panel of 28 genes, as previously reported. 30 Therefore, residual LBC specimens from the solid organs such as the thyroid gland and lymph node would be very useful for molecular testing of cancers. As…”

Section: Discussionmentioning

confidence: 99%

Next‐generation sequencing in residual liquid‐based cytology specimens for cancer genome analysis

Yamaguchi

Akahane

Harada

et al. 2020

Diagnostic Cytopathology

View full text Add to dashboard Cite

Background: Cancer genome profiling of cytology specimens using next-generation sequencing (NGS) requires adequate and good-quality DNA. Genomic examination of cytology samples was conventionally performed on cell block (CB) or smear specimens than on residual liquid-based cytology (LBC) specimens, which are high-quality DNA sources even after long-term storage. Methods: We estimated tumor fractions of 37 residual LBC specimens, including 30 fine needle aspiration (FNA) samples from the thyroid (12 papillary thyroid carcinomas and two malignant lymphomas), lymph node (13 metastatic carcinomas and one malignant lymphoma), and breast cancer (one phyllodes tumor and one invasive ductal carcinoma), two pancreatic carcinoma samples, and five liquid (ascites, pleural effusion, and cerebrospinal fluid) samples. The DNA was extracted from all samples and subjected to NGS using a customized cancer gene panel comprising 28 cancerrelated genes. Results: NGS analysis revealed somatic mutations corresponding to pathological diagnosis with adequate variant allele frequency (VAF) in 24 LBC specimens, which had significantly higher tumor fraction (72.5% ± 4.9%). Ten cases, including the five fluid samples, had very small tumor fractions (7.5% ± 2.3%) to obtain sufficient VAF. Other two samples had high tumor fractions but showed very low VAF, indicating the presence of fusion genes. The remaining one sample yielded no DNA recovery. Conclusion: The residual LBC specimens collected by FNA from the thyroid gland and lymph node were verified to carry high tumor fraction and could serve as an alternate source for molecular testing to screen and diagnose cancers without the use of CB or smears.

show abstract

Section: Discussionmentioning

confidence: 99%

Next‐generation sequencing in residual liquid‐based cytology specimens for cancer genome analysis

Yamaguchi

Akahane

Harada

et al. 2020

Diagnostic Cytopathology

View full text Add to dashboard Cite

show abstract

“…Bipolar coagulation is appreciated for a clear surgical field and small trauma. However, the difficulty of treatment due to the surgical site and the high psychological pressure of the patients may hinder the prognosis without proper nursing intervention [ 7 , 8 ]. Nursing interventions can effectively reduce risk events, improve self-management efficacy, and alleviate adverse emotions of thyroid cancer patients, leading to fewer adverse reactions [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of High-Quality Nursing Management on Thyroid Tumor Patients after Bipolar Coagulation

Zhang

et al. 2022

Journal of Oncology

View full text Add to dashboard Cite

Objective. To evaluate the effect of high-quality nursing on thyroid tumor patients after bipolar coagulation and its impact on nursing satisfaction. Methods. From January 2019 to January 2020, 90 patients with thyroid tumors treated with bipolar coagulation in our hospital were identified as the study objects and randomized to the control group ( n = 45 ) and the experimental group ( n = 45 ) random number table method. Both groups received conventional nursing, and the experimental group additionally adopted high-quality nursing. The incidence of postoperative complications and nursing satisfaction between the two groups was compared. The Exercise of Self-Care Agency Scale (ESCA) was used to assess the self-care ability of patients after the intervention, and the Generic Quality of Life Inventory-74 (GQOLI-74) was used to evaluate the quality of life of the two groups of patients after the intervention. The Hospital Anxiety and Depression (HAD) scale was adopted to evaluate the emotional state of the patient before and after the intervention, and the Numerical Rating Scale (NRS) was employed to evaluate the pain intensity of the patient after the intervention. Counting data was analyzed by the X2 test, and the measurement data was analyzed by t-test. Results. After the intervention, the total incidence of postoperative complications in the experimental group was significantly lower than that in the control group (11.11% versus 33.33%, P < 0.05 ). The experimental group obtained remarkably higher nursing satisfaction (93.33% versus 71.11%), ESCA scores, and GQOLI-74 scores than the control group ( P < 0.05 ). Lower HAD scores were observed in the experimental group than those of the control group ( P < 0.001 ). Lower HAD scores were observed in the experimental group than those of the control group ( P < 0.001 ). The NRS score of the experimental group was significantly lower than that of the control group 12 h and 24 h after the intervention ( P < 0.001 ). Conclusion. High-quality nursing for patients with thyroid tumors can effectively alleviate the negative emotions, improve the quality of life, and contribute to a harmonious nurse-patient relationship, which is worthy of promotion and application.

show abstract

“…Of note, genetic alterations are detected in approximately 90% of papillary thyroid carcinomas (PTCs), and more than 60% of thyroid malignancies [ 13 - 22 ]. Therefore, in order to further risk-stratify patients with indeterminate thyroid cytology diagnoses, molecular studies have been utilized to detect genetic alterations and play an important role in the therapeutic triage of thyroid lesions [ 13 , 14 ]. Molecular tests demonstrate a high negative predictive value (92%-96%) in thyroid nodules [ 13 , 14 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Do ACR TI-RADS scores demonstrate unique thyroid molecular profiles?

et al. 2022

View full text Add to dashboard Cite

Purpose: Our study aimed to examine the molecular profiles of cytologically indeterminate thyroid nodules stratified by American College of Radiology (ACR) Thyroid imaging reporting and data system (TI-RADS) categories and to determine whether certain ultrasonographic features display particular molecular alterations. Methods: We performed a retrospective review of cases from 1/1/2016-4/1/2018. Cases with in-house ultrasound, fine needle aspiration (FNA) Thyroid Bethesda Reporting System for Cytology (TBSRTC) diagnoses, molecular testing, and surgery were included. All cases were diagnosed as TBSRTC indeterminate categories. Ultrasound studies were retrospectively reviewed and assigned TI-RADS scores (TR1-TR5) by board-certified radiologists. Final diagnoses were determined based on the surgical resection pathology. Binary logistic regression analysis was used to study whether demographics, TI-RADS levels and TBSRTC diagnoses were associated with ThyroSeq molecular results. Results: 81 cases met the inclusion criteria. The most common alteration across all TI-RADS categories was RAS mutation (TR2 2/2; TR3 10/19, TR4 13/44, and TR5 8/16), which did not stratify with any particular TI-RADS category. Only TR4 and TR5 categories displayed more aggressive mutations such as BRAF-V600E and TERT. ThyroSeq results were positively correlated with thyroid malignancy when non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) was categorized in the malignant category for outcome (OR 6.859; p<0.01), but not in the Thyroid Malignancy category when NIFTP was removed from the malignancy category. Echogenicity scores were found to be negatively correlated with ThyroSeq results in thyroid nodules (OR 0.162; P<0.01). Conclusions: Higher risk molecular alterations tended to stratify with the higher TI-RADS categories.

show abstract

Diagnostic accuracy of molecular testing with three molecular markers on thyroid fine‐needle aspiration cytology with abnormal category

Cited by 9 publications

References 28 publications

Next‐generation sequencing in residual liquid‐based cytology specimens for cancer genome analysis

Next‐generation sequencing in residual liquid‐based cytology specimens for cancer genome analysis

The Effect of High-Quality Nursing Management on Thyroid Tumor Patients after Bipolar Coagulation

Do ACR TI-RADS scores demonstrate unique thyroid molecular profiles?

Contact Info

Product

Resources

About