Diagnostic ability of salivary matrix metalloproteinase‐9 lateral flow test point‐of‐care test for periodontitis

Kim, Hyun-Duck; Lee, Chang‐Soo; Cho, Hyun-Jae; Jeon, Sumin; Choi, Youngnim; Kim, Sung-Tae; Kim, DanHee; Lee, Hyun Jin; Vu, Huong; Jeong, Hee‐Jin; Kim, ByungGee

doi:10.1111/jcpe.13360

Cited by 16 publications

(11 citation statements)

References 27 publications

(49 reference statements)

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“…This test may therefore be usefully incorporated in a multi‐test strategy. Consistent with these findings, recent data showed that the use of an MMP‐9 point‐of‐care test in conjunction with demographic and lifestyle behavioural factors could facilitate the detection of periodontitis (Kim et al, 2020 ). Building upon and expanding the above understandings, the aMMP‐8 POCT might be used in combination with other sensitive screening methods for detecting periodontitis.…”

Section: Discussionmentioning

confidence: 59%

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Diagnostic accuracy of a point‐of‐care aMMP‐8 test in the discrimination of periodontal health and disease

Deng

Pelekos

Jin

et al. 2021

J Clinic Periodontology

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Aim: To assess the diagnostic utility of an oral rinse active matrix metalloproteinase-8 (aMMP-8) point-of-care test (POCT) for differentiating periodontal health, gingivitis, as well as different stages and grades of periodontitis. Materials & Methods:The aMMP-8 index test was undertaken in 408 consecutive adults, followed by a full-mouth periodontal examination. The reference standard was the 2017 World Workshop classification of periodontal diseases. Sensitivity, specificity, and the area under the receiver operating characteristic curve (AUROC) were assessed.Results: 68.6% of the participants were diagnosed with periodontitis, including Stages I (15.9%), II (15.9%), III (29.7%) and IV (7.1%). A positive aMMP-8 POCT was associated with periodontitis after adjusting for age, gender, tobacco smoking and systemic diseases, while it was unable to differentiate among the stages/grades of periodontitis and between gingivitis/periodontal health. This test showed a sensitivity of 33.2% and a specificity of 93.0% for detecting periodontitis (threshold level >10 ng/ml). The levels of aMMP-8 adjusted by the number of teeth present (aMMP-8/NTP) performed better for periodontitis (sensitivity: 67.1%; specificity: 68.8%). Notably, aMMP-8/NTP were strongly predictive for Stage IV periodontitis (threshold level =0.4312 ng/ml) (sensitivity: 89.7%; specificity: 73.6%; and AUROC: 0.856). The test performance greatly improved in combination with age and smoking, with a sensitivity of 82.5%, a specificity of 84.4%, and an AUROC of 0.883. Conclusion:This aMMP-8 POCT is able to detect periodontitis with better specificity than sensitivity across the spectrum of its severity. This test may be useful for periodontal screening in conjunction with subject characteristics and/or other sensitive screening tools. Further validation studies are needed. K E Y W O R D Sdiagnosis, gingivitis, matrix metalloproteinase-8, periodontal health, periodontitis, point-ofcare test, screening, sensitivity and specificity Clinical RelevanceScientific rationale for the study: The traditional periodontal diagnostic approach relies on clinical parameters that are difficult to measure, lack precision to detect incipient periodontitis and can only reflect the previous tissue destruction. Point-of-care biomarker tests based on oral fluidsThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Section: Discussionmentioning

confidence: 59%

“…age and tobacco smoking) of periodontitis were combined. This test may therefore be usefully incorporated (Kim et al, 2020). Building upon and expanding the above understandings, the aMMP-8 POCT might be used in combination with other sensitive screening methods for detecting periodontitis.…”

Section: 9%mentioning

confidence: 99%

Diagnostic accuracy of a point‐of‐care aMMP‐8 test in the discrimination of periodontal health and disease

Deng

Pelekos

Jin

et al. 2021

J Clinic Periodontology

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“…To date, clinical studies have been reported using lateral flow assays to identify bronchiectasis severity, airway infection, risk of exacerbations 208 and periodontitis. 209 Preliminary clinical validation has identified point-of-care paper-based biosensors to be an efficient tool for screening diseases and identifying bronchiectasis patients at high risk of disease exacerbations. 208…”

Section: Discussionmentioning

confidence: 99%

Point-of-care COPD diagnostics: biomarkers, sampling, paper-based analytical devices, and perspectives

Dong

Yang

et al. 2022

Analyst

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“…Periodontal tissue destruction has been associated with the presence of high levels of active MMP-9 in gingival tissue, saliva, and gingival crevicular fluid [47][48][49]. Moreover, this MMP may be a practical marker for assessing the clinical severity of periodontitis [50]. We thus used MMP-9 as an MMP model to investigate the ability of eriodictyol to inhibit the catalytic activity of MMPs.…”

Section: Discussionmentioning

confidence: 99%

Eriodictyol Suppresses Porphyromonas gingivalis-Induced Reactive Oxygen Species Production by Gingival Keratinocytes and the Inflammatory Response of Macrophages

Maquera-Huacho

Spolidorio

Manthey

et al. 2022

Front. Oral. Health

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Porphyromonas gingivalis is a key pathogen of periodontitis, an inflammatory disease that affects the tooth-supporting tissues. The aim of the present study was to investigate the effects of the flavanone eriodictyol on P. gingivalis-induced reactive oxygen species (ROS) production by gingival keratinocytes and the inflammatory response of macrophages. Porphyromonas gingivalis and H2O2 acted synergistically to induce ROS production by keratinocytes. The presence of eriodictyol significantly attenuated ROS production in a dose-dependent manner. We used a macrophage model to show that eriodictyol decreases the secretion of IL-1β, IL-6, IL-8, and TNF-α induced by P. gingivalis. Evidence has been brought that this anti-inflammatory property of eriodictyol may be related to its ability to prevent the activation of the NF-κB signaling pathway by P. gingivalis. This periodontal pathogen was also found to be a potent inducer of matrix metalloproteinase (MMP) production by macrophages, including MMP-2, MMP-8, and MMP-9. Eriodictyol dose-dependently inhibited the production of all three MMPs. Lastly, eriodictyol inhibited the catalytic activity of both MMP-9 and P. gingivalis collagenase. In conclusion, eriodictyol may be a potential therapeutic agent for preventing and/or treating periodontal disease due to its antioxidant, anti-inflammatory, and anti-proteinase properties.

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Diagnostic ability of salivary matrix metalloproteinase‐9 lateral flow test point‐of‐care test for periodontitis

Cited by 16 publications

References 27 publications

Diagnostic accuracy of a point‐of‐care aMMP‐8 test in the discrimination of periodontal health and disease

Diagnostic accuracy of a point‐of‐care aMMP‐8 test in the discrimination of periodontal health and disease

Point-of-care COPD diagnostics: biomarkers, sampling, paper-based analytical devices, and perspectives

Eriodictyol Suppresses Porphyromonas gingivalis-Induced Reactive Oxygen Species Production by Gingival Keratinocytes and the Inflammatory Response of Macrophages

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