Diagnosis to Detect Porcine Transmissible Gastroenteritis Virus (TGEV) by Optical and Transmission Electron Microscopy Techniques

Martins, A. M. C. R. P. F.; Bersano, Josete Garcia; Ogata, Renato Akio; Amante, G; Nastari, B.D.B; Catroxo, Márcia Helena Braga

doi:10.4067/s0717-95022013000200059

Cited by 14 publications

(16 citation statements)

References 31 publications

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“…As a notifiable disease, the TGEV causes significant economic losses in the pig industry and has been reported in several countries as Europe, American and Asia [105][106][107]. TGEV was detected for the first time in Brazil through histopathological techniques and the transmission electron microscopy in 19 (25.3%) small intestine samples of pigs from various municipalities in the State of São Paulo and the Minas Gerais, Brazil [108].…”

Section: Porcine Coronavirus 1221 Porcine Transmissible Gastroentmentioning

confidence: 99%

“…The disease affects pigs of all ages, and symptoms were represented by severe watery diarrhea accompanied by vomiting [109,110], anorexia, prostration, dehydration, dyspnea and death [108].…”

Section: Porcine Coronavirus 1221 Porcine Transmissible Gastroentmentioning

confidence: 99%

“…Resin embedding technique allowed to study several ultrastructural aspects of the intracellular behavior of the TGEV in intestinal fragments of infected pigs [108] and of the parvovirus in intestinal fragments of newborn dogs with diarrhea [220].This technique also allows studying the efficiency of the vaccines based on in situ produced, noninfectious rotavirus-like particles (RVLPs) [221].…”

Section: Resin Embedding Techniquementioning

confidence: 99%

“…To confirm the viral strain (TGEV), immunoelectron microscopy (IEM) (Figure 8) and immunolabeling techniques with gold particles, performed with a monoclonal antibody specific for TGEV, can be used. Viral ultrastructural aspects can be studied through the resin embedding technique (Figure 9) [108].…”

Section: Porcine Coronavirus 1221 Porcine Transmissible Gastroentmentioning

confidence: 99%

“…This technique was used to label TGEV particles in feces and small intestine fragments of infected pigs [108], type A rotavirus and coronavirus in samples from diarrheic calves and winter dysenteric cattle [143], the simultaneous presence of coronavirus and rotavirus in feces of calves with diarrhea [211] and BVDV in feces of cattle with diarrhea [184].…”

Section: Immunolabeling With Colloidal Gold Particles By Negative-stamentioning

confidence: 99%

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Application of Transmission Electron Microscopy Techniques in the Veterinary Diagnosis of Viral Gastroenteritis in Livestock Animals

Catroxo¹,

Martins²

2018

Stomach Disorders

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Gastroenteritis caused by viruses is considered to be one of the most important diseases in livestock, being the main cause of morbidity and mortality in young animals, culminating in serious economic losses due to costs with prophylaxis and treatment, increased susceptibility of animals to secondary infections, developmental delay and death. Stressful factors may support the onset of illness. Several viral agents can cause gastroenteritis in various animal species. Rotaviruses are considered the main cause of enteric infections in various animals, including humans constituting important zoonosis. Due to genetic diversity and their ability to cross the species barrier, the coronaviruses infect many species. In cattle, they cause "Winter Dysentery" in adult animals and "Neonatal Diarrhea" in newborn calves. In swine, they are responsible for "Transmissible Gastroenteritis" and "Swine Epidemic Diarrhea." Equines infected with coronavirus also develop severe gastroenteritis. Bovine viral diarrhea (BVD) caused by a flavivirus of the genus Pestivirus is related to digestive and reproductive disorders, affecting any productive sector, are it cut, milk or confinement. Transmission electron microscopy is an indispensable tool in the diagnosis of viral gastroenteric infectious diseases. Negative staining is a simple, fast and efficient technique, being ideal for the detection of gastroenteric viruses, being easily visualized. The immunoelectron microscopy (IEM) technique allows increasing the sensitivity of virus detection where low concentrations of virus are aggregated so that they may be more easily seen. The immunolabeling with colloidal gold technique utilizes specific antibodies tagged with particles of colloidal gold to label the antigen antibody reaction. Embedding resin technique allows obtaining information on the virus-cell interaction. The different transmission electron microscopy modalities promotes a fast and accurate diagnosis of the different gastroenteric viral agents, allowing prophylactic measures of control and prevention in the creations to be promptly instituted, avoiding animal losses and disastrous economic losses, and collaborating with the National Porcine and Bovine Agribusiness.

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Section: Porcine Coronavirus 1221 Porcine Transmissible Gastroentmentioning

confidence: 99%

Section: Porcine Coronavirus 1221 Porcine Transmissible Gastroentmentioning

confidence: 99%

Section: Resin Embedding Techniquementioning

confidence: 99%

Section: Porcine Coronavirus 1221 Porcine Transmissible Gastroentmentioning

confidence: 99%

Section: Immunolabeling With Colloidal Gold Particles By Negative-stamentioning

confidence: 99%

See 3 more Smart Citations

Application of Transmission Electron Microscopy Techniques in the Veterinary Diagnosis of Viral Gastroenteritis in Livestock Animals

Catroxo¹,

Martins²

2018

Stomach Disorders

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Proteomic Analysis of IPEC‐J2 Cells in Response to Coinfection by Porcine Transmissible Gastroenteritis Virus and Enterotoxigenic Escherichia coli K88

Dai

Zhu

et al. 2017

Proteomics Clinical Apps

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Prospects for the use of spherical gold nanoparticles in immunization

Староверов

Volkov

Mezhenny

et al. 2018

Appl Microbiol Biotechnol

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Recent years have seen extremely fast development of new viral nanovaccines and diagnostic agents using nanostructures prepared by biological and chemical synthesis. We used spherical gold nanoparticles (average diameter, 15 nm) as a platform for the antigen for swine transmissible gastroenteritis virus (TGEV). The literature data demonstrate that immunization of animals with the TGEV antigen coupled to gold nanoparticles (GNPs) not only activates antigenpresenting cells but also increases the proliferative activity of splenic lymphoid (antibody-forming) cells. The contents of γ-IFN, IL-1β, and IL-6 in animals immunized with GNP-antigen conjugates were found to be higher than those in intact animals or in animals given the antigen alone. The increased concentration of IL-1β in the immunized animals directly correlated with the activity of macrophages and stimulated B cells, which produce this cytokine when activated. The increased concentration of IL-6 indicates that the injected preparations are stimulatory to cellular immunity. Immunization with the TGEV antigen conjugated to GNPs as a carrier activates the respiratory activity of lymphoid cells and peritoneal macrophages, which is directly related to their transforming activity and to the activation of antibody generation. Furthermore, the use of this conjugate allows marked improvement of the structure of the animals' immune organs and restores the morphological-functional state of these organs. The microanatomical changes (increased number of follicles) indicate the activation of the B-dependent zone of the spleen and, consequently, the development of a humoral-type immunological reaction. The degradative processes observed in the animals immunized with TGEV antigen alone are evidence of weak resistance to pathogen attack. These results can be used to develop vaccines against this infection by employing TGEV antigen coupled to gold nanoparticles as a carrier.

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Diagnosis to Detect Porcine Transmissible Gastroenteritis Virus (TGEV) by Optical and Transmission Electron Microscopy Techniques

Abstract: Diagnosisto detect porcine transmissible gastroenteritis virus (TGEV) by optical and transmission electron microscopy techniques.

Cited by 14 publications

References 31 publications

Application of Transmission Electron Microscopy Techniques in the Veterinary Diagnosis of Viral Gastroenteritis in Livestock Animals

Application of Transmission Electron Microscopy Techniques in the Veterinary Diagnosis of Viral Gastroenteritis in Livestock Animals

Proteomic Analysis of IPEC‐J2 Cells in Response to Coinfection by Porcine Transmissible Gastroenteritis Virus and Enterotoxigenic Escherichia coli K88

Prospects for the use of spherical gold nanoparticles in immunization

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