Diagnosis of Tuberculosis

Bates, Joseph H.

doi:10.1378/chest.76.6_supplement.757

Cited by 83 publications

(29 citation statements)

References 39 publications

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“…To control TB, the rapid, accurate diagnosis of TB is very important. The diagnosis of TB is based on the clinical findings, chest radiography, detection of acidfast bacilli (AFB) in smears, and mycobacterial culture (Bates, 1979). The detection of AFB in smears is a simple, rapid method but has poor sensitivity and cannot differentiate M. tuberculosis from other AFBpositive bacteria, such as non-tuberculous mycobacteria (NTM), Nocardia spp., and Rhodococcus spp.…”

Section: Introductionmentioning

confidence: 99%

Development of a highly sensitive one-tube nested real-time PCR for detecting Mycobacterium tuberculosis

Choi

Jeon

Shim

et al. 2014

Diagnostic Microbiology and Infectious Disease

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Section: Introductionmentioning

confidence: 99%

Development of a highly sensitive one-tube nested real-time PCR for detecting Mycobacterium tuberculosis

Choi

Jeon

Shim

et al. 2014

Diagnostic Microbiology and Infectious Disease

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“…The criteria for a definitive diagnosis of GTB by demonstration of Mycobacterium tuberculosis are hardly ever met in people with paucibacillary GTB [6], because microscopy for acid-fast bacilli (AFB) requires the presence of at least 10 000 bacilli/mL of specimen and culture requires at least 100 bacilli/mL [7]. Because there is no gold standard, the diagnosis of GTB presents a dilemma to clinicians and there remains a high index of suspicion.…”

Section: Introductionmentioning

confidence: 99%

Genital tuberculosis among infertile women and fertility outcome after antitubercular therapy

Kulshrestha

Kriplani

Agarwal

et al. 2011

Intl J Gynecology & Obste

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“…Staining of acid-fast bacilli (AFB) to identify Mycobacterium tuberculosis takes less than 1 hour to perform, but is relatively insensitive and requires about 10 4 organisms per milliliter of specimen for a positive result [1]. Moreover, this method lacks specificity.…”

Section: Introductionmentioning

confidence: 99%

Multicenter Study of a Commercial, Automated Polymerase Chain Reaction System for the Rapid Detection of Mycobacterium tuberculosis in Respiratory Specimens in Routine Clinical Practice

Bogard¹,

Vincelette

Antinozzi

et al. 2001

Eur J Clin Microbiol Infect Dis

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A cooperative study was conducted among six laboratories to compare the performance of the Cobas Amplicor (CA) polymerase chain reaction (PCR) system (Roche Molecular Systems, USA) for the detection of Mycobacterium tuberculosis with that of microscopy and culture in routine clinical laboratory diagnosis. A total of 5,221 decontaminated respiratory specimens were tested. The use of an internal control allowed detection of PCR inhibition in 144 (2.8%) specimens. Only two culture-positive samples were CA PCR inhibitory and therefore could not be detected by PCR testing. Of the 333 culture-positive specimens, 278 (83.5%) were positive by the CA PCR. Of the 4,744 culture-negative specimens, 52 (1.1%) were positive by the CA PCR. After analysis of discrepancies, 40 of the 52 culture-negative, CA PCR-positive specimens were classified as true positive. Thus, the overall sensitivities of culture, CA PCR and microscopy were 89.3%, 85.2% and 55.5%, respectively. The overall specificity of the CA PCR was 99.7%. Five of the six centers found similar performances for the CA PCR, with sensitivities ranging from 85.7 to 90.9%. The CA PCR was more sensitive for smear-positive samples, exhibiting overall sensitivities of 96.1% and 71.7% for smear-positive and smear-negative specimens, respectively. These results indicate that the Cobas Amplicor system enables microbiology laboratories with reasonable previous experience in molecular biology testing to perform PCR and to detect Mycobacterium tuberculosis in more than 70% of specimens obtained from infected patients.

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Diagnosis of Tuberculosis

Cited by 83 publications

References 39 publications

Development of a highly sensitive one-tube nested real-time PCR for detecting Mycobacterium tuberculosis

Development of a highly sensitive one-tube nested real-time PCR for detecting Mycobacterium tuberculosis

Genital tuberculosis among infertile women and fertility outcome after antitubercular therapy

Multicenter Study of a Commercial, Automated Polymerase Chain Reaction System for the Rapid Detection of Mycobacterium tuberculosis in Respiratory Specimens in Routine Clinical Practice

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