Diagnosis of Trypanosoma cruzi Infection

Luquetti, Alejandro O.; Schmuñis, Gabriel A.

doi:10.1016/b978-0-12-384876-5.00028-9

Cited by 8 publications

(15 citation statements)

References 100 publications

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“…Tais métodos têm utilidade complementar, e devem sempre ser realizados em casos suspeitos ou confirmados de doença de Chagas aguda. 39,134 Nos casos em que não se identifica o parasito na pesquisa direta, a verificação da presença de anticorpos anti-T. cruzi da classe IgM no sangue periférico é considerada sugestiva da fase aguda, particularmente quando associada a contexto epidemiológico e manifestações clínicas. 129,135 Representa uma técnica de maior complexidade de realização, mais adequada na fase aguda tardia, quando as repetições dos exames de pesquisa direta tiverem resultados negativos.…”

Section: Critério Parasitológicounclassified

II Consenso Brasileiro em Doença de Chagas, 2015

Dias¹,

Ramos²,

Gontijo³

et al. 2016

Epidemiol. Serv. Saúde

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A doença de Chagas é uma condição crônica negligenciada com elevada carga de morbimortalidade e impacto dos pontos de vista psicológico, social e econômico. Representa um importante problema de saúde pública no Brasil, com diferentes cenários regionais. Este documento traduz a sistematização das evidências que compõe o Consenso Brasileiro de Doença de Chagas. O objetivo foi sistematizar estratégias de diagnóstico, tratamento, prevenção e controle da doença de Chagas no país, de modo a refletir as evidências científicas disponíveis. Sua construção fundamentou-se na articulação e contribuição estratégica de especialistas brasileiros com conhecimento, experiência e atualização sobre diferentes aspectos da doença. Representa o resultado da estreita colaboração entre a Sociedade Brasileira de Medicina Tropical e o Ministério da Saúde. Espera-se com este documento fortalecer o desenvolvimento de ações integradas para enfrentamento da doença no país com foco em epidemiologia, gestão, atenção integral (incluindo famílias e comunidades), comunicação, informação, educação e pesquisas.

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Section: Critério Parasitológicounclassified

II Consenso Brasileiro em Doença de Chagas, 2015

Dias¹,

Ramos²,

Gontijo³

et al. 2016

Epidemiol. Serv. Saúde

View full text Add to dashboard Cite

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“…For instance, the infection rates of T. cruzi in Amazonian monkeys ranged from 10.3% using parasitological methods (Ziccardi and Lourenco-de-Oliveira 1997; Ziccardi et al 2000) to 46% using serology (Lisboa et al 2006). Furthermore, in studies using PCR several other aspects must be taken into account, such as sample type, sample volume and conservation, DNA extraction method, parasite sequences targeted, primers, reagents, and thermocycling conditions (Luquetti and Schmuñis 2010). …”

Section: Discussionmentioning

confidence: 99%

“…Dried blood spot sampling overcame the limitations of cold chain in rural areas and remote locations (Luquetti and Schmuñis 2010). However, natural inhibitors present in samples and the uneven distribution of parasite DNA on FTA cards may decrease the assay’s sensitivity (Pizarro et al 2007; Ahmed et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…However, the number of cases is probably underestimated as the disease is often asymptomatic. Transmission typically occurs via a triatomine bug vector, but it can also occur via blood transfusion, organ transplantation, congenital transmission, the oral route, and accidental infection (Luquetti and Schmuñis 2010). Recent outbreaks reported in Latin America have been caused by foodborne transmission of T. cruzi due to ingestion of contaminated beverages (Yoshida 2008; Coura and Junqueira 2015).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Epidemiology of Trypanosomatids and Trypanosoma cruzi in Primates from Peru

Aysanoa

Mayor

Mendoza³

et al. 2017

EcoHealth

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We determined the prevalence rate and risk of infection of Trypanosoma cruzi and other trypanosomatids in Peruvian non-human primates (NHPs) in the wild (n = 126) and in different captive conditions (n = 183). Blood samples were collected on filter paper, FTA cards, or EDTA tubes and tested using a nested PCR protocol targeting the 24Sα rRNA gene. Main risk factors associated with trypanosomatid and T. cruzi infection were genus and the human–animal context (wild vs captive animals). Wild NHPs had higher prevalence of both trypanosomatids (64.3 vs 27.9%, P < 0.001) and T. cruzi (8.7 vs 3.3%, P = 0.057), compared to captive NHPs, suggesting that parasite transmission in NHPs occurs more actively in the sylvatic cycle. In terms of primate family, Pitheciidae had the highest trypanosomatid prevalence (20/22, 90.9%) and Cebidae had the highest T. cruzi prevalence (15/117, 12.8%). T. cruzi and trypanosomatids are common in Peruvian NHPs and could pose a health risk to human and animals that has not been properly studied.

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“…All serology was carefully performed with at least four different techniques of different principles. Inconclusive results, usually observed in 1.4% of serological tests for detection of T. cruzi infection ( Luquetti & Schmuñis 2010 ), were excluded. When the concentration of antibodies was low in one of the tests, aliquots of sera were separated and all tests were performed again.…”

Section: Discussionmentioning

confidence: 99%

Congenital transmission of Trypanosoma cruzi in central Brazil. A study of 1,211 individuals born to infected mothers

Luquetti

Tavares

Siriano

et al. 2015

Mem. Inst. Oswaldo Cruz

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Transmission of Trypanosoma cruzi during pregnancy is estimated to occur in less than 20% of infected mothers; however, the etiopathogenesis is not completely understood. The Centre for Studies on Chagas Disease provides confirmation of T. cruzi infection for individuals living in central Brazil. In this retrospective hospital-based study, all requests for diagnosis of T. cruzi infection in individuals less than 21 years old from 1994-2014 were searched. We end with 1,211 individuals and their respective infected mothers. Congenital transmission of infection was confirmed in 24 individuals (2%) in central Brazil, an area where the main T. cruzi lineage circulating in humans is TcII. This low prevalence of congenital Chagas disease is discussed in relation to recent findings in the south region of Brazil, where TcV is the main lineage and congenital transmission has a higher prevalence (approximately 5%), similar to frequencies reported in Argentina, Paraguay and Bolivia. This is the first report to show geographical differences in the rates of congenital transmission of T. cruzi and the relationship between the prevalence of congenital transmission and the type of Tc prevalent in each region.

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Diagnosis of Trypanosoma cruzi Infection

Cited by 8 publications

References 100 publications

II Consenso Brasileiro em Doença de Chagas, 2015

II Consenso Brasileiro em Doença de Chagas, 2015

Molecular Epidemiology of Trypanosomatids and Trypanosoma cruzi in Primates from Peru

Congenital transmission of Trypanosoma cruzi in central Brazil. A study of 1,211 individuals born to infected mothers

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