Diagnosis of Sarcocystis spp. in cattle (Bos taurus) and water buffalo (Bubalus bubalis) in Northern Vietnam

Jehle, C.; Dinkel, Anke; Sander, A.; Morent, M.; Römig, Thomas; Luc, Pham Van; De, Tyler; Thai, V.V.; Mackenstedt, Ute

doi:10.1016/j.vetpar.2009.08.024

Cited by 66 publications

(64 citation statements)

References 17 publications

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“…However, both species were reported from water buffaloes worldwide including China (Li et al, 2002;Yang et al, 2001b). Vietnam (Jehle et al, 2009) and Iran (Oryan et al, 2011). Huong (1999a;1999b) reported four species Sarcocystis, S. levinei, S. dubeyi, S. buffalonis and S. fusiformis in Vietnam.…”

Section: Ajavsmentioning

confidence: 99%

“…Recently, the 18S rRNA genes have been extensively used as suitable targets for the accurate identification of the closely related species of Sarcocystis as well as phylogenetic analyses (Holmdahl et al, 1999;Li et al, 2002;Dahlgren and Gjerde, 2007;Jehle et al, 2009). The conserved regions of 18S rRNA gene help in designing primers that can be used to amplify the same Science Publications AJAVS gene in related species.…”

Section: Ajavsmentioning

confidence: 99%

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Molecular Characterization of Sarcocytis Fusiformis and Sarcocystis Buffalonis Infecting Water Buffaloes (Bubalus Bubalis) From Egypt

El-Seify¹

2014

American Journal of Animal and Veterinary Sciences

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Parasites of the genus Sarcocystis have the ability to infect wide range of animals, birds and reptiles. Water buffaloes are intermediate hosts for four Sarcocystis species; S. fusiformis, S. buffalonis, S. levinei and S. dubeyi. There was no previous molecular identification of Sarcocystis spp. parasitizing water buffaloes from Egypt. The current study was established to screen infection rates and apply genetic identification of the macroscopic Sarcocystis spp. infecting Egyptian water buffalos in El-Gharbia province, Egypt. Sarcocysts of both S. fusiformis and S. buffalonis were grossly examined through meat inspection in slaughterhouse, followed by histological identification of the detected sarcocysts. Finally, molecular identification using PCR, sequencing and phylogenetic analysis of the 18S rRNA gene was conducted. Comparison of the obtained sequences of the local tested isolates of both S. fusiformis and S. buffalonis with those previously reported for different Sarcocystis species those were recorded in Genbank, revealed that all the tested specimens represented both S. fusiformis and S. buffalonis. Moreover, sequence alignments of the partial 18S rRNA gene ascertained the presence of genetic variations revealing the heterogenic nature of the gene among different strains of the same Sarcocystis species in relation to the geographic distribution.

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Section: Ajavsmentioning

confidence: 99%

Section: Ajavsmentioning

confidence: 99%

Molecular Characterization of Sarcocytis Fusiformis and Sarcocystis Buffalonis Infecting Water Buffaloes (Bubalus Bubalis) From Egypt

El-Seify¹

2014

American Journal of Animal and Veterinary Sciences

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“…There is a debate whether the Sarcocystis species in cattle and buffaloes are the same species (Jehle et al 2009;Chen et al 2011;Gjerde, 2013;Dubey et al 2014;Moré et al 2014). However, there is general agreement that S. fusiformis of the water buffalo is distinctive and has no morphological equivalent in cattle.…”

Section: Introductionmentioning

confidence: 99%

Redescription of Sarcocystis fusiformis sarcocysts from the water buffalo (Bubalus bubalis)

et al. 2014

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S U M M A R YFour valid species of Sarcocystis have been reported from the water buffalo (Bubalus bubalis): Sarcocystis fusiformis, Sarcocystis buffalonis, Sarcocystis levinei and Sarcocystis dubeyi. Here, we redescribe structure of S. fusiformis sarcocysts by scanning and transmission electron microscopy (SEM, TEM). Twenty-one macroscopic sarcocysts from oesophagus of the water buffalo in Egypt were examined by light microscopy, SEM and TEM. The sarcocyst wall was up to 9 μm thick, depending on the section and the technique. In 5 μm paraffin-embedded sections, the sarcocyst wall was indistinct, 2-5 μm thick and appeared smooth. In 1 μm plastic-embedded sections stained with toluidine blue, the sarcocyst wall was 2·5-5·2 μm thick and had branched villar protrusions (vp)-like branches of a dead tree. By SEM, the sarcocyst wall had a mesh-like structure with irregularly shaped vp that were folded over the sarcocyst wall. On each vp there were uniform papillomatous structures that were 100 nm wide. By TEM, vp were up to 6 μm long and contained filamentous tubular structures, most of which were parallel to the long axis of the projections; granules were absent from these tubules. By TEM, bradyzoites within the same cyst varied from 11·2 to 16·8 μm in length. By TEM, bradyzoites had a very long (10 μm) convoluted mitochondrion, up to 12 dense granules, but only 2 rhoptries. This redescription should help to differentiate the sarcocysts of S. fusiformis from similar sarcocysts in domestic and wild ruminants.

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“…We noted the presence of two types of cysts with a 4.1% frequency in diaphragms. Many studies have revealed the presence of double infestations within one pet, in sheep [6]; the Asian buffalo [38] but also cattle. Indeed, Harhoura et al [2] noted the presence of mixed infections as well as in the esophagus in the diaphragm with respective frequencies of 5% and 19.3%.…”

Section: Mixed Infectionsmentioning

confidence: 99%

Study of the Bovine Sarcosporidiosis in the Slaughterhouses of the North of Algeria: Case of the Slaughterhouses of El Harrach (Algiers)

Aissi¹

2016

Cell Dev Biol

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The aim of our study was the determination of prevalence of the sarcosporidiosis in the bovine carcasses in the slaughterhouses of El Harrach and the identification of involved species of Sarcocystis. Samples of oesophagi and diaphragms of 200 cattles brought down in the slaughterhouse of El Harrach were analyzed by the histo-pathological technique and the technique of digestion pepsique. We did not note macroscopic cyst in the carcasses inspection. The enzymatic digestion and the histo-pathological analysis revealed high rates of infestations (95%) and (80%) respectively. Cysts with thin wall of S. cruzi were dominant in diaphragms (94.2%) and in oesophagi (100%). A low rate of cysts with thick wall was detected in diaphragms (4.4%) and a rate of 0% in oesophagi. A single cyst of S. hominis was able to be diagnosed by photonique microscope in a diaphragm.

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Diagnosis of Sarcocystis spp. in cattle (Bos taurus) and water buffalo (Bubalus bubalis) in Northern Vietnam

Cited by 66 publications

References 17 publications

Molecular Characterization of Sarcocytis Fusiformis and Sarcocystis Buffalonis Infecting Water Buffaloes (Bubalus Bubalis) From Egypt

Molecular Characterization of Sarcocytis Fusiformis and Sarcocystis Buffalonis Infecting Water Buffaloes (Bubalus Bubalis) From Egypt

Redescription of Sarcocystis fusiformis sarcocysts from the water buffalo (Bubalus bubalis)

Study of the Bovine Sarcosporidiosis in the Slaughterhouses of the North of Algeria: Case of the Slaughterhouses of El Harrach (Algiers)

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