Diagnosis of psoroptic sheep scab with an improved enzyme-linked immunosorbent assay

Ochs, H.; Lonneux, J. F.; Losson, Bertrand; Deplazes, Peter

doi:10.1016/s0304-4017(00)00444-1

Cited by 30 publications

(28 citation statements)

References 20 publications

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“…Hence, purified recombinant allergen is becoming increasingly used for diagnosis as a substitute for whole mite extracts . Crude Psoroptes antigens have been used in serodiagnosis to detect Psoroptes infections . In recent years, however, there has been a trend towards using recombinant protein antigens as the basis for ELISA assays as these were reported to be capable of detecting subclinically infested animals .…”

Section: Discussionmentioning

confidence: 99%

Evaluating troponin C from Psoroptes cuniculi as a diagnostic antigen for a dot‐ELISA assay to diagnose mite infestations in rabbits

Zheng

Zhang

et al. 2014

Parasite Immunology

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The mite Psoroptes cuniculi is globally widespread and has a serious impact on commercial rabbit breeding. In China, diagnosis of P. cuniculi is currently based on conventional clinical methods that entail numerous disadvantages, including their failure to diagnose subclinical infections. Hence, alternative measures are required, and dot-ELISA is one of the most promising strategies. We cloned and expressed the recombinant P. cuniculi troponin C gene for use as a basis for novel dot-ELISA assay to detect P. cuniculi infections in rabbits. This amplified sequence encoded a 153 amino acid protein of 17·6 kDa and theoretical pI 4·18 without signal peptide. The recombinant troponin C of P. cuniculi is an outer membrane protein and may also be a new P. cuniculi allergen. Results of dot-ELISA test showed that this novel assay had more than 90% sensitivity but low specificity in distinguishing infections with P. cuniculi or Sarcoptic scabiei, despite very high agreement between observers (97-99%; κ values ranged from 0·95 to 0·98 for inter- and intra-observer variability test). This study showed that this novel method, at present, lacks diagnostic utility. Therefore, although simple serological assays such as dot-ELISA show great promise as diagnostic tools, we suggest that troponin C is not a suitable diagnostic antigen candidate.

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Section: Discussionmentioning

confidence: 99%

Evaluating troponin C from Psoroptes cuniculi as a diagnostic antigen for a dot‐ELISA assay to diagnose mite infestations in rabbits

Zheng

Zhang

et al. 2014

Parasite Immunology

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“…Various workers have earlier indicated an adverse effect of psoroptic mange on the immune system of sheep (Fisher, 1972; Boyce and Zarnke, 1996; Matthes et al., 1996; Ochs et al., 2001; Kenyon and Knox, 2002) and cattle (Losson et al., 1988; Uhlir, 1989) but no report could be traced with detailed effect of sarcoptic mange on CMI and HI responses in sheep. The present work indicated an adverse effect of infection on both cell‐mediated and humoral immunity.…”

Section: Discussionmentioning

confidence: 99%

Effects of Sarcoptic Mange and its Control with Oil of Cedrus deodara, Pongamia glabra, Jatropha curcas and Benzyl Benzoate, both with and without Ascorbic Acid on Growing Sheep: Epidemiology; Assessment of Clinical, Haematological, Cell‐Mediated Humoral Immune Responses and Pathology

Dimri

Sharma²

2004

Journal of Veterinary Medicine Series A

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The aim of this study was to evaluate the therapeutic efficacy of commonly used acaricidal drugs in India and also to assess the effect of ascorbic acid (AA) as adjunct therapy in 72 growing sheep with sarcoptic mange, aged 5-6 months and weighing 20.4-31.7 kg. Eight replicates of nine animals were formed based on sex, and day 0 body weight. Another set of 12 healthy sheep (5-6 months old) constituted the healthy control group. Drugs were applied locally on the affected parts daily and recovery changes in skin lesions were observed at the time of every application. L-Ascorbic acid was administered intramuscularly. Skin scrapings were collected daily from each group and examined for the presence of mites. Blood samples from each group were collected and analysed for total erythrocytes, leucocytes, haematocrit, haemoglobin and lymphocytes on 0, 14th and 28th day post-treatment (PT). Cell-mediated (CMI) and humoral immune (HI) responses were assessed on 0 and 28 days PT. Within groups treated with the drug alone, recovery in oil of Jatropha curcas group was faster and lesions were free from mites after the eighth application. For each drug, the recovery was faster with AA as an adjunct therapy when compared with the group treated with the drug alone. Within all groups, recovery in oil of J. curcas plus ascorbic acid (OJC-AA) group was fastest and the lesions were mite-free after three applications. The overall mean values were higher for all haematological parameters in OJC-AA group. Recovery with respect to suppressed CMI and HI responses during infection was also faster in this group. Gross and microscopic pathological studies revealed a marked adverse effect of infection on skin, kidney, spleen and liver. The OJC-AA group indicated faster recovery. It is concluded from this study that oil of J. curcas was found more efficacious in controlling sarcoptic mange in sheep and AA might be beneficial as adjunct in case of sarcoptic mange to get quick recovery with lesser applications of main therapy. However, the pharmacology of AA with reference to health of skin needs to be investigated.

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“…Targeted treatments of P. ovis -infested sheep, based on flock serology, have been used successfully to reduce the incidence of sheep scab [9,10]. Our group has recently developed a novel diagnostic enzyme linked immunosorbent assay (ELISA) capable of accurately detecting P. ovis infestation in sheep [11].…”

Section: Introductionmentioning

confidence: 99%

The use of a Psoroptes ovis serodiagnostic test for the analysis of a natural outbreak of sheep scab

et al. 2012

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BackgroundSheep scab is a highly contagious disease of sheep caused by the ectoparasitic mite Psoroptes ovis. The disease is endemic in the UK and has significant economic impact through its effects on performance and welfare. Diagnosis of sheep scab is achieved through observation of clinical signs e.g. itching, pruritis and wool loss and ultimately through the detection of mites in skin scrapings. Early stages of infestation are often difficult to diagnose and sub-clinical animals can be a major factor in disease spread. The development of a diagnostic assay would enable farmers and veterinarians to detect disease at an early stage, reducing the risk of developing clinical disease and limiting spread.MethodsSerum samples were obtained from an outbreak of sheep scab within an experimental flock (n = 480 (3 samples each from 160 sheep)) allowing the assessment, by ELISA of sheep scab specific antibody prior to infestation, mid-outbreak (combined with clinical assessment) and post-treatment.ResultsAnalysis of pre-infestation samples demonstrated low levels of potential false positives (3.8%). Of the 27 animals with clinical or behavioural signs of disease 25 tested positive at the mid-outbreak sampling period, however, the remaining 2 sheep tested positive at the subsequent sampling period. Clinical assessment revealed the absence of clinical or behavioural signs of disease in 132 sheep, whilst analysis of mid-outbreak samples showed that 105 of these clinically negative animals were serologically positive, representing potential sub-clinical infestations.ConclusionsThis study demonstrates that this ELISA test can effectively diagnose sheep scab in a natural outbreak of disease, and more importantly, highlights its ability to detect sub-clinically infested animals. This ELISA, employing a single recombinant antigen, represents a major step forward in the diagnosis of sheep scab and may prove to be critical in any future control program.

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Diagnosis of psoroptic sheep scab with an improved enzyme-linked immunosorbent assay

Cited by 30 publications

References 20 publications

Evaluating troponin C from Psoroptes cuniculi as a diagnostic antigen for a dot‐ELISA assay to diagnose mite infestations in rabbits

Evaluating troponin C from Psoroptes cuniculi as a diagnostic antigen for a dot‐ELISA assay to diagnose mite infestations in rabbits

Effects of Sarcoptic Mange and its Control with Oil of Cedrus deodara, Pongamia glabra, Jatropha curcas and Benzyl Benzoate, both with and without Ascorbic Acid on Growing Sheep: Epidemiology; Assessment of Clinical, Haematological, Cell‐Mediated Humoral Immune Responses and Pathology

The use of a Psoroptes ovis serodiagnostic test for the analysis of a natural outbreak of sheep scab

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